Crenubiotus liangshuiensis, Zhang & Sun & Wang & Hao & Ma & Zhao & Li & Zhao & Yang, 2024

Zhang, Jing-Yu, Sun, Xue-Ling, Wang, Ning, Hao, Li, Ma, Cheng-Xue, Zhao, Na, Li, He- Ping, Zhao, Min & Yang, Sheng-Tao, 2024, Tardigrades in the alpine region of Northeast China with an integrative description of Crenubiotus liangshuiensis sp. nov., Zootaxa 5492 (1), pp. 96-108 : 100-102

publication ID	https://doi.org/ 10.11646/zootaxa.5492.1.5
publication LSID	lsid:zoobank.org:pub:336B4DA3-553E-4EAA-9277-272D386378DB
DOI	https://doi.org/10.5281/zenodo.13212585
persistent identifier	https://treatment.plazi.org/id/03E3878C-867F-FFBC-FF7D-E738FDB45E96
treatment provided by	Plazi
scientific name	Crenubiotus liangshuiensis
status	sp. nov.

Treatment

Crenubiotus liangshuiensis sp. nov. ( Figs 2–6 View FIGURE 2 View FIGURE 3 View FIGURE 4 View FIGURE 5 View FIGURE 6 , Table 2 View TABLE 2 )

Material examined: 11 specimens and 1 egg mounted on microscope slides in Hoyer’s medium, 1 specimen fixed on a SEM stub, and 12 specimens processed for DNA sequencing.

Type locality: 47º11′03.8″N, 128º53′45.6″E; 416 m asl: China, Yichun , Liangshui National Nature Reserve. Substrate: moss on fallen logs .

Etymology: The new species is named after the Liangshui National Nature Reserve, where it was discovered.

Type depositories: The holotype, 10 paratypes (slide Nos. NMS0011–21 View Materials ) and 1 egg ( NMS0022 View Materials ) are deposited in the Physiology Lab , College of Wildlife and Protected Area , Northeast Forestry University, Harbin, China .

Description of the new species. Length up to 291 µm, colourless, eyes present. Body cuticle with numerous irregular pores ( Fig. 2 View FIGURE 2 ), large quadrangular pores up to 1.2×2.9 µm in diameter ( Fig. 3B View FIGURE 3 ) and small, elliptical pores about 0.5 µm in diameter, all evident in PCM and NCM, as well as in SEM. The small elliptical pores are distributed randomly on the entire body cuticle ( Fig. 2 View FIGURE 2 ), and the larger irregular quadrangular pores are mostly distributed in the middle of each segment, forming 9–10 transverse bands on the dorsum ( Fig. 2B View FIGURE 2 ), slightly larger in size on the caudal portion of the body. Pore bands clearly distinguishable under LCM. Evident bulges are present on the external and lateral portions of the leg I–III surface ( Fig. 3C View FIGURE 3 , Fig. 4A View FIGURE 4 ). The granulation is present on all legs (e.g. Fig. 4E View FIGURE 4 ). Small cuticular tubercles are present in a dorso-lateral caudal band just anterior to the hind legs ( Fig. 3D View FIGURE 3 ) and on the dorsal cuticle of legs IV, with some sparse tubercles present on the internal side of legs IV ( Fig. 3A View FIGURE 3 ). Claws are slender, of the richtersiusid type ( Fig. 4 View FIGURE 4 ). Primary branches with distinct accessory points, a long, common tract with a system of internal septa, the common tract apparently longer than half of the entire claw height.

The bucco-pharyngeal apparatus is of the Macrobiotus type, pharynx with triangular apophyses, two rodshaped macroplacoids (2<1), and a microplacoid close to the second macroplacoid ( Fig. 5 View FIGURE 5 ). The first macroplacoid is anteriorly narrowed and constricted in the middle, whereas the second has a subterminal constriction.

The egg is white, spherical, with conical processes comprising a main body and a distal elongated and flexible portion ( Fig. 6 View FIGURE 6 ). Some process apices are bifurcated and may contain internal septa forming a single distal bubble ( Fig. 6D View FIGURE 6 ). The single egg found has a diameter of 73.4 µm including the processes and 52.1 µm without them; there are about 16 processes on the circumference. The processes are 12.2 µm high and have a basal diameter of 9.4 µm (average values of four processes).