Heterohartmannula undetermined

Pan, Hongbo, Lin, Xiaofeng, Gong, Jun, Al-Rashied, Khaled A. S. & Song, Weibo, 2012, Taxonomy of five species of cyrtophorids (Protozoa: Ciliophora) including consideration of the phylogeny of two new genera, Zoological Journal of the Linnean Society 164 (1), pp. 1-17 : 3-6

publication ID	https://doi.org/ 10.1111/j.1096-3642.2011.00751.x
persistent identifier	https://treatment.plazi.org/id/03E08903-E956-FF89-4B74-FA48FD31FEC2
treatment provided by	Carolina
scientific name	Heterohartmannula undetermined
status	SP.

Treatment

HETEROHARTMANNULA FANGI SP. NOV.

( FIGS 1 View Figure 1 , 2 View Figure 2 ; TABLE 1)

Diagnosis: Marine Heterohartmannula c. 60–90 ¥ 20– 30 Mm in vivo, body oval-shaped in outline; pellicle of cilium-free field covered by densely arranged bacteria; 28–32 ventral kineties, the right-most six of which extend apically; about 19 nematodesmal rods; preoral kinety usually composed of three fragments; about six contractile vacuoles ventrally located; a yellowish pigment spot near the anterior tip of cell; podite subterminally positioned.

Type deposition: The holotype slide of protargolimpregnated specimens is deposited in the Laboratory of Protozoology, Ocean University of China, China (No. PHB10040701 ). A paratype slide is in the National History Museum, London, UK (registration number 2010:11:7:2) .

Type locality: Coastal waters off Donghai Island, Zhanjiang (21°14′N, 110°23′E), China (temperature 24 °C, salinity 27‰) GoogleMaps .

Dedication: We dedicate this new species to our respected colleague, Prof. Dr Jingyun Fang, Peking University, China, in recognition of his tremendous contributions to the fields of biodiversity and plant ecology.

Description: Cell size about 60–90 ¥ 20–30 Mm in vivo, body highly flexible and slightly contractile, usually long oval in outline. Right margin more convex than left, with anterior ‘beak’ projecting to left; usually ventral surface flat and dorsal surface vaulted in midbody ( Figs 1A, B, F View Figure 1 , 2A–E View Figure 2 ). Pellicle of ciliumfree field conspicuously covered with densely arranged bacteria ( Fig. 2F View Figure 2 ). Podite about 7 Mm in length, subterminally positioned. Cytostome slitlike, surrounded by 17–22 straight tooth-tipped nematodesmal rods, extending posteriorly slightly leftwards ( Figs 1G View Figure 1 , 2H View Figure 2 ). Cytoplasm colourless or greyish, usually with numerous granules (1–2 Mm across), which render the cell more or less opaque. One small patch-like pigment spot positioned at anterior left of cell, usually yellowish to dark yellow in colour ( Fig. 2H View Figure 2 , arrowhead). About six contractile vacuoles (4 Mm in diameter), most of which are longitudinally arranged on ventral side ( Figs 1A View Figure 1 , 2C View Figure 2 ). Cilia about 7 Mm long. Movement slow, usually crawling on substrate.

Infraciliature as shown in Figures 1C–E, G, H View Figure 1 , 2G, I–L View Figure 2 . 28–32 somatic kineties, the right-most six to seven of which extend apically. Ten to 12 right, five to nine postoral, and 11–13 left kineties surrounding the oral area; left and postoral kineties posteriorly shortened progressively from right to left ( Figs 1G, H View Figure 1 , 2G, L View Figure 2 ). Terminal fragment ( Fig. 1G View Figure 1 ) consisting of three to eight basal bodies; equatorial fragment not detected. About nine kinetosome-like dots present near the base of podite (arrowhead in Fig. 1H View Figure 1 and arrow in Fig. 2L View Figure 2 ). Macronucleus ellipsoidal, heteromerous, about 18 ¥ 11 Mm in size. Micronucleus invisible.

Two circumoral kineties obliquely arranged, the right of which is sometimes divided into two fragments (three of 22 individuals) ( Figs 1C–E View Figure 1 , 2J–L View Figure 2 ), and they seldom connect with each other (one of 22 individuals) ( Fig. 2G View Figure 2 ). Preoral kineties usually comprising two to four fragments ( Figs 1C–E View Figure 1 , 2J–L View Figure 2 ).

Comparison: Considering general morphology (e.g. cell size and shape, numbers of somatic kineties and contractile vacuoles), Heterohartmannula fangi sp. nov. resembles Hartmannula angustipilosa . However, it can be separated from the latter by obliquely (vs. in parallel) arranged circumoral kineties, the number of nematodesmal rods (17–22 vs. ten–11) and the presence of bacteria on the pellicle (vs. absence) ( Gong & Song, 2004b; Table 2).

Hartmannula sinica Shao et al. 2008 is similar to Heterohartmannula fangi sp. nov. in terms of body size, numbers of somatic kineties, and nematodesmal rods ( Shao et al., 2008). Nevertheless, Hartmannula sinica can be clearly identified by having an alveolar layer on its cell surface (vs. absence in latter) and more contractile vacuoles (c. 15 vs. c. six) (Table 2).

Heterohartmannula fangi sp. nov. has the same number of nematodesmal rods as Hartmannula acrobates Poche, 1913 . However, it differs from the latter by the character of cell surface (partly covered by bacteria vs. by an alveolar layer), and number of somatic kineties (28–32 vs. 32–37) ( Deroux & Dragesco, 1968; Table 2).

Except for differences in oral kineties, Hartmannula derouxi Gong et Song, 2004 , differs from

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Kingdom	Chromista
Phylum	Ciliophora
Class	Cyrtophoria
Order	Cyrtophorida
Family	Hartmannulidae
Genus	Heterohartmannula