Hemicycliophora undetermined

Subbotin, Sergei A., Chitambar, John J., Chizhov, Vlamidir N., Stanley, Jason D., Inserra, Renato N., Doucet, Marcelo E., Clure, Michael M, Ye, Weimin, Yeates, George W., Mollov, Dimitre S., Cantalapiedra-Navarrete, Carolina, Vovlas, Nicola, Berg, Esther Van Den & Castillo, Pablo, 2014, Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae), Zoological Journal of the Linnean Society 171 (3), pp. 475-506 : 493

HEMICYCLIOPHORA SP. 11

( FIG. 2C, H, M; TABLES 1, S8)

This species from Florida is characterized by a ventrally arcuate body; cuticular sheath loosely fitting and attached to body only at anterior end and vulva; annulation coarse, lateral fields marked by irregularities, breaks, and some anastomoses. Outside lateral field annuli smooth, lip region hemispherical to slightly rounded with three annuli: first annulus narrower than succeeding ones, separate, somewhat anteriorly directed, labial disc slightly elevated or not. Stylet straight or curved; basal knobs rectangular to elongate rounded, posteriorly sloped, with distinct, large cavity. Vulval lips modified, about 1.5 annuli long. Spermatheca oval, without sperm. Postvulval body contracted immediately posterior to vulva. Tail tapers gradually, then more abruptly posteriorly to an elongate conical narrower portion with a finely rounded, more or less distinctly annulated terminus. No males were found for this species .

The non-identified Hemicycliophora sp. 11 is similar to H. thienemanni , H. similis , and H. vaccinii . It differs from H. thienemanni and H. similis by the number of labial annuli (three vs. two), shape of first labial annulus (separate from other labial annuli and anteriorly directed vs. not separate and laterally directed), and fitting of cuticular sheath (loosely fitting vs. very closely adpressed to body). It further differs from H. thienemanni by a slightly shorter stylet (65–80 vs. 76–105 μm) and contour of the postvulval body immediately behind the vulva (contracted vs. not contracted). It differs from H. similis by a shorter stylet (65–80 vs. 88–108 μm), shorter body (700–978 vs. 920– 1578 μm), and the presence of a large stylet knobs cavity vs. very small or absent. From H. vaccinii it differs by a shorter stylet (65–80 vs. 84–112 μm), smaller RV (50–67 vs. 70–76), VL/VB (2.7–4.6 vs. 4.7–9.0), shape of labial region (rounded vs. truncate), and vulval lips (modified, elongate vs. not modified, not elongate). This species is clearly different from the above-mentioned species in the ITS and D2-D3 of 28S rRNA gene sequences.

Figures

Figure 2. Photomicrographs of specimens of populations of selected Hemicycliophora species. A–E, anterior region; F–J, lateral field: K–O, posterior region. A, F, K, Hemicycliophora floridensis (topotype, Florida, USA); B, G, L, Hemicycliophora poranga (California, USA); C, H, M, Hemicycliophora sp. 11 (Florida, USA), D, I, N, Hemicycliophora sp. 4 (North Carolina, USA); E, J, O, Hemicycliophora wyei (North Carolina, USA). Scale bars: A–E, K–O = 10 μm; F–J = 5 μm.