Robertozyma ningxiaensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 117

Robertozyma ningxiaensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828826. Fig. 15O .

Etymology: the specific epithet ningxiaensis refers to the geographic origin of the type strain, Ningxia province, China.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are oval and ellipsoidal, 3.2– 4.5 × 3.9–6.8 μm and single, budding is polar ( Fig. 15O ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange red, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), trehalose, glycerol, D-mannitol, salicin (delayed and weak) and succinate are assimilated as sole carbon sources. L-sorbose, sucrose, maltose, cellobiose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, erythritol, ribitol, galactitol, Dglucitol, Methyl-α- D-glucoside, D-gluconate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine and ethylamine hydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 22 –23 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Helanshan mountain , Ningxia province, obtained from soil, Aug. 2009, P.J. Han (holotype CGMCC 2.4451 T preserved in a metabolically inactive state, ex-type CBS 12499 = HLS10.23).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.