Phyllozyma jiayinensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 113

Phyllozyma jiayinensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828814. Fig. 15C .

Etymology: the specific epithet jiayinensis refers to the geographic origin of the type strain, Jiayin, Heilongjiang.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 1.4–2.0 × 3.2 –7.3 μm and single, budding is polar ( Fig. 15C ), a sediment is present. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and somewhat wrinkled and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, trehalose, D-mannitol, Dglucitol (delayed), D-gluconate (weak) and DL-lactate (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, sucrose, maltose, cellobiose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, salicin, citrate, succinate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate (delayed and weak) are assimilated as sole nitrogen sources. Sodium nitrite, Llysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Phy. jiayinensis and its closely related species Phy. dimennae and Phy. corallina are distinguishable from one another by assimilation of sucrose, D-xylose, glycerol, ribitol, Dglucitol, Methyl-α- D-glucoside, DL-lactate, succinate and sodium nitrite ( Table S1.22 ).

Typus: China, Qingshan county, Jiayin , Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2014, Q.-M. Wang (holotype CGMCC 2.5669 T preserved in a metabolically inactive state, ex-type CBS 13975 = HLJ25.21).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.