Oberwinklerozyma nepetae Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 126

Oberwinklerozyma nepetae Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828848. Fig. 18I .

Etymology: the specific epithet nepetae refers to Nepeta , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, ellipsoidal and ovoid, 2.7– 3.2 × 6.4– 8.9 μm and single, budding is polar ( Fig. 18I ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is white cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, D-mannitol, Dglucitol, Methyl-α- D-glucoside and salicin are assimilated as sole carbon sources. Galactose, lactose, inulin, soluble starch, Dxylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, Dglucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, O. nepetae differs from its closely related species O. yarrowii and O. silvestris in its inability to assimilate galactose, D-glucosamine, ethanol, glycerol, DL-lactate, succinate, citrate and myo-inositol ( Table S1.33 ).

Typus: China, Motuo , Tibet, obtained from a leaf of Nepeta sp. , Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5824 T preserved in a metabolically inactive state, ex-type CBS 15579 = XZ129C7).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.