Rhodosporidiobolus jianfalingensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 123-124

Rhodosporidiobolus jianfalingensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828840. Fig. 18A, B .

Etymology: the specific epithet jianfalingensis refers to the geographic origin of the type strain, Jianfaling, Hainan.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 1.4–2.9 × 4.3–10.0 μm and single, budding is polar ( Fig. 18A ), a sediment is present. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pale cream, butyrous, slightly wrinkled and glossy. The margin is entire or eroded. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.1–2.9 × 5.0– 7.1 μm ( Fig. 18B ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose (weak), melibiose (weak), raffinose, melezitose, soluble starch, D-xylose, L-arabinose, D-arabinose (weak), Dribose (weak), L-rhamnose, D-glucosamine (weak), Methyl-α- Dglucoside, salicin, succinate (weak) and citrate (weak) are assimilated as sole carbon sources. L-sorbose, inulin, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, DL-lactate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite and L-lysine are assimilated as sole nitrogen sources. Ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 25 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Rh. jianfalingensis differs from its closely related four species, Rh. platycladi , Rh. nylandii , Rh. odoratus and Rh. ruineniae , in its inability to assimilate L-sorbose, glycerol, ribitol, D-mannitol and D-glucitol ( Table S1.30 ).

Typus: China, Jianfaling , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3532 T preserved in a metabolically inactive state, ex-type CBS 15494 = JF25.7-1).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.