Colacogloea aletridis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 131-132

Colacogloea aletridis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828862. Fig. 19N .

Etymology: the specific epithet aletridis refers to Aletris , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and ovoid, 2.0–3.8 × 3.0– 7.6 μm and single, budding is polar ( Fig. 19N ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose (weak), trehalose, melezitose, ethanol, ribitol (delayed), D-mannitol (weak), D-glucitol (weak) and Methyl-α- D-glucoside (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, cellobiose, lactose, melibiose, raffinose, inulin, soluble starch, Dxylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, Dglucosamine, methanol, glycerol, erythritol, galactitol, salicin, DLlactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 30 Ί C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Co. aletridis differ well from other Colacogloe species in its assimilation of carbon and nitrogen sources ( Table S1.38 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Aletris pauciflora, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2766 T preserved in a metabolically inactive state, ex-type CBS 15459 = XZ31A1).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.