Chrysozyma cylindrica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 129-130

Chrysozyma cylindrica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828856. Fig. 19E, F .

Etymology: the specific epithet cylindrica refers to the cylindrical vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 2.2– 3.2 × 3.9– 10.0 μm and single, budding is polar ( Fig. 19E ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and semi-glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 1.5– 2.5 × 3.8– 6.3 μm ( Fig. 19F ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delay and weak), sucrose, trehalose (delay), melezitose, D-mannitol and D-glucitol are assimilated as sole carbon sources. L-sorbose, maltose, cellobiose, lactose, melibiose, raffinose, inulin, soluble starch, Dxylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, Dglucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and Llysine are not assimilated. Maximum growth temperature is 22– 23 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. cylindrica differs well from other Chrysozyma species in its assimilation of carbon and nitrogen sources ( Table S1.34 ).

Typus: China, Wuzhishan mountain , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3455 T preserved in a metabolically inactive state, ex-type CBS 15482 = WZS29.2).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.