Chrysozyma sorbariae Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 129

Chrysozyma sorbariae Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828855. Fig. 19C, D .

Etymology: the specific epithet sorbariae refers to Sorbaria , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are elongate ellipsoidal and cylindrical, 1.7–2.7 × 5.8–10.4 μm and single, budding is polar ( Fig. 19C ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and semi-gloosy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or falcate, 2.1–2.9 × 6.4–7.9 μm ( Fig. 19D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, trehalose, melezitose, inulin (delayed and weak), D-mannitol and D-glucitol are assimilated as sole carbon sources. Galactose, Lsorbose, cellobiose, lactose, melibiose, raffinose, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak) and sodium nitrite are assimilated as sole nitrogen sources. Llysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26 –27 Ί C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. sorbariae differs well from other Chrysozyma species in its assimilation of carbon and nitrogen sources ( Table S1.34 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Sorbaria arboricola, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2768 T preserved in a metabolically inactive state, ex-type CBS 15460 = XZ9D1).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.