Chrysozyma fusiformis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 129

Chrysozyma fusiformis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828854. Fig. 19A, B .

Etymology: the specific epithet fusiformis refers to the fusiform vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal to fusiform, 3.0– 4.6 × 4.7– 8.2 μm and single, budding is polar ( Fig. 19A ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and dull surface. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal orallantoid, 2.9– 4.3 × 7.1 –11.4 μm ( Fig. 19B ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, melibiose, melezitose, ethanol, D-mannitol and succinate (delayed and weak) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, raffinose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, Dglucosamine, methanol, glycerol, erythritol, ribitol, galactitol, Dglucitol, Methyl-α- D-glucoside, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 24 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. fusiformis differs well from other Chrysozyma species in its assimilation of carbon and nitrogen sources ( Table S1.34 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2004, F.-Y. Bai (holotype CGMCC 2.2765 T preserved in a metabolically inactive state, ex-type CBS 15458 = XZ33C2).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.