Pseudohyphozyma hydrangeae Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 130

Pseudohyphozyma hydrangeae Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828858. Fig. 19H .

Etymology: the specific epithet hydrangeae refers to Hydrangea , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 3.0– 4.3 × 5.8– 9.1 μm and single, budding is polar ( Fig. 19H ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, maltose, cellobiose, trehalose, melezitose, inulin (variable), soluble starch (variable), D-xylose (variable), L-arabinose (variable), D-arabinose (variable), ethanol, ribitol, D-mannitol, D-glucitol and succinate (variable) are assimilated as sole carbon sources. Galactose, L-sorbose, sucrose, lactose, melibiose, raffinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 29 °C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ps. hydrangeae and its four closely related species, Ps. lulangensis , Ps. bogoriensis , Ps. pustula and Ps. buffonii , can be distinguished from one another by the assimilation of galactose, L-sorbose, melezitose, glycerol, salicin, citrate, potassium nitrate and sodium nitrite ( Table S1.35 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of Hydrangea heteromalla, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2796 T preserved in a metabolically inactive state, ex-type CBS 15462 = XZ46A1).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.