Chrysozyma flava Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 130

Chrysozyma flava Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828857. Fig. 19G .

Etymology: the specific epithet flava refers to the yellow colony colour of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal or to cylindrical, 2.1– 3.1 × 4.0– 10.8 μm and single, budding is polar ( Fig. 19G ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellow, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, melezitose, ethanol, D-mannitol and D-gluconate (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, raffinose, inulin, soluble starch, D-xylose, Larabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, D-glucitol, Methyl-α- D-glucoside methanol, glycerol, erythritol, ribitol, galactitol, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate is assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. flava differs well from other Chrysozyma species in its assimilation of carbon and nitrogen sources ( Table S1.34 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5611 T preserved in a metabolically inactive state, ex-type CBS 15552 = GPS20.4A1).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.