Kockovaella nitrophila Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 83

Kockovaella nitrophila Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828739. Fig. 7E .

Etymology: the specific epithet nitrophila refers to the physiological character of assimilating nitrate.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ellipsoidal, 2.4 –4.4 × 3.7– 4.5 μm and single, budding is polar ( Fig. 7E ), a sediment is formed. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is creamish white, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (weak), sucrose, maltose, trehalose, melibiose (weak), raffinose, melezitose, inulin, Dxylose (weak), L-arabinose (weak), D-arabinose (weak), Dribose (weak) and DL-lactate (weak) are assimilated as sole carbon sources. L-sorbose, cellobiose, lactose, soluble starch, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine (weak), ethylamine hydrochloride (weak) and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 37 °C. Growth in vitamin-free medium is positive (weak). Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive. The major ubiquinone is Q-10.

Physiologically, Koc. nitrophila differs from its five closely related species, Koc. ischaemi , Koc. haikouensis , Koc. sacchari , Koc. thailandica and Koc. imperatae , in its inability to assimilate cellobiose, melibiose, D-glucosamine, N-Acetyl-D-glucosamine and D-mannitol and its ability to assimilate potassium nitrate ( Table S1.1 ).

Typus: China, Wuzhishan mountain , Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3465 T preserved in a metabolically inactive state, ex-type CBS 15487 = WZS12.1).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.