Vishniacozyma pseudopenaeus Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 84-85

Vishniacozyma pseudopenaeus Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828743. Fig. 7I .

Etymology: the specific epithet pseudopenaeus refers to the similar colony morphology and physiological characteristics to that of Vishniacozyma penaeus .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subsphaeroidal and ellipsoidal, 2.6 –3.5 × 2.8– 5.0 μm and single, budding is polar ( Fig. 7I ), a sediment is formed. After 1 mo at 17 °C, a pellicle and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale grayish-cream, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch (varialbe), D-xylose, L-arabinose, Darabinose, D-ribose, L-rhamnose, D-glucosamine, D-gluconate, ethanol (varialbe), glycerol, erythritol (varialbe), ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin,, DL-lactate (varialbe), succinate (weak), citrate and myo-inositol are assimilated as sole carbon sources. Inulin, methanol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (variable), L-lysine, ethylamine hydrochloride (weak) and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Sodium nitrite are not assimilated as sole nitrogen sources. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is positive. Starch-like substances are produced or not. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, V. pseudopenaeus differs from the closely related species V. penaeus in its ability to grow in vitamin-free medium, however, the latter does not grow in vitamin-free medium ( Table S1.4 ).

Typus: Germany, obtained from a leaf of an unidentified plant, Sep. 2005 (holotype CGMCC 2.3165 T preserved in a metabolically inactive state, ex-type CBS 15472 = G7.20).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.