Bulleribasidium phyllophilum Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 91-93

Bulleribasidium phyllophilum Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828768. Fig. 8O, P .

Etymology: the specific epithet phyllophilum refers to leaves, the substrate origin of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.0–4.0 × 4.0– 9.3 μm and single, budding is polar ( Fig. 8O ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is prey-cream, butyrous, smooth and semi-glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal, subglobosal to napiform, 3.8– 6.2 × 4.6– 6.2 μm ( Fig. 8P ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, inulin (variable), soluble starch (variable), D-xylose, L-arabinose, Darabinose, L-rhamnose, D-glucosamine (weak), N-Acetyl-D-glucosamine (variable), galactitol, D-mannitol, D-glucitol (variable), Methyl-α- D-glucoside (delayed and weak) and myo-inositol (variable) are assimilated as sole carbon sources. Lsorbose, lactose, D-ribose, methanol, ethanol, glycerol, erythritol, ribitol, salicin, DL-lactate, succinate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (variable), L-lysine (variable) and ethylamine hydrochloride (variable) are assimilatedas sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Bu. phyllophilum and its closely related species Bu. foliicola cannot be distinguished from each other. The former did not grow at 30 °C, but the latter grew weak ( Table S1.10 ).

Typus: China, Bangxi county, Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3320 T preserved in a metabolically inactive state, ex-type CBS 15474 = HBX2.8).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.