Bulleribasidium cremeum Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 91

Bulleribasidium cremeum Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828766. Fig. 8K, L .

Etymology: the specific epithet cremeum refers to the pale-cream colony morphology.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 1.7– 4.8 × 4.5 –8.7 μm and single, budding is polar ( Fig. 8K ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale-cream, butyrous, smooth and semi-glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal to napiform, 3.3– 6.7 × 4.0– 6.7 μm ( Fig. 8L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose (delayed and weak), trehalose, melibiose, raffinose, melezitose, D-xylose (delayed and weak), L-arabinose (delayed and weak), D-arabinose, salicin (weak) and succinate are assimilated as sole carbon sources. L-sorbose, lactose, inulin, soluble starch, D-ribose, L-rhamnose, Dglucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, DLlactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and L-lysine are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Bu. cremeum differs from its closely related species, Bu. phyllostachydis , Bu. wuzhishanense and Bu. setariae , in its inability to assimilate galactitol, D-mannitol and Methyl-α- D-glucoside ( Table S1.10 ).

Typus: China, Taiwan province, obtained from a leaf of an unidentified plant, Aug. 2009, Q.-M. Wang (holotype CGMCC 2.4427 T preserved in a metabolically inactive state, ex-type CBS 12487 = TW1.1F-025).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.