Dioszegia ovata Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 89-90

Dioszegia ovata Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828761. Fig. 8E, F .

Etymology: the specific epithet ovata refers to the ovoid cell morphology of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.3–4.6 × 3.8– 7.7 μm and single, budding is polar ( Fig. 8E ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pink to orange, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are globosal and subglobosal to napiform, 3.1–6.2 × 3.8– 6.9 μm ( Fig. 8F ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose (delayed), sucrose, maltose, cellobiose, trehalose, lactose (delayed), melibiose, raffinose, melezitose, soluble starch, D-xylose, Larabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine (delayed and weak), galactitol, D-mannitol, Methyl-α- D-glucoside, salicin (weak) and succinate (delayed and weak) are assimilated as sole carbon sources. Inulin, methanol, ethanol, glycerol, erythritol, ribitol, D-glucitol, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate (delayed and weak) are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is positive. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Di. ovata and the closely related species Di. maotaiensis , Di. kandeliae , Di. zsoltii , Di. catarinoi , Di. takashimae and Di. athyrii can be distinguished from one another. Di. ovata differs from the other six species in its ability to grow at 32 °C ( Table S1.9 ).

Typus: China, Bangxi county, Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3625 T preserved in a metabolically inactive state, ex-type CBS 15657 = HBX1.27).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.