Kondoa cylindrica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 103-104

Kondoa cylindrica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828792. Fig. 12C, D .

Etymology: the specific epithet cylindrica refers to the cylindrical ballistoconidia of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 2.4–4.8 × 4.8– 8.5 μm and single, budding is polar ( Fig. 12C ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale orange, butyrous, smooth and semi-glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are cylindrical, 2.1– 2.9 × 4.3–5.7 μm ( Fig. 12D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose (variable), trehalose, raffinose (variable), melezitose (variable), soluble starch, D-xylose (variable), L-arabinose (variable), D- ribose (variable), L-rhamnose, ethanol (variable), glycerol, erythritol (variable), ribitol (variable), galactitol (variable), Dmannitol, D-glucitol, Methyl-α- D-glucoside (variable), salicin (variable), succinate (delayed and weak) and citrate (variable) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, inulin, D-arabinose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, D-gluconate, DL-lactate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (variable) and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 22–25 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. cylindrica differs from its closely related species Kon. aeria and Kon. malvinella in its inability to assimilate DL-lactate and its ability to grow in vitamin-free medium ( Table S1.17 ).

Typus: Germany, obtained from a leaf of an unidentified plant, Sep. 2005 (holotype CGMCC 2.3102 T preserved in a metabolically inactive state, ex-type CBS 15466 = G6.1-1).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.