Kondoa chamaenerii Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 104

Kondoa chamaenerii Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828793. Fig. 12E, F .

Etymology: the specific epithet chamaenerii refers to Chamaenerion , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 2.6–4.3 × 5.7–10.0 μm and single, budding is polar ( Fig. 12E ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pinkish-cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are long ellipsoidal, 2.9– 4.3 × 7.1– 10.0 μm ( Fig. 12F ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), L-sorbose (variable), sucrose, maltose, cellobiose (variable), trehalose, lactose (variable), raffinose (variable), inulin (weak), soluble starch (variable), glycerol, ribitol (delayed and weak), mannitol (delayed and weak) and D-glucitol (variable) are assimilated as sole carbon sources. Melibiose, melezitose, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, erythritol, galactitol, D-Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. chamaenerii . differs from its closely related species Kon. subrosea and Kon. miscanthi in its inability to assimilate succinate ( Table S1.17 ).

Typus: China, Bujin county, Xinjiang province, obtained from a leaf of Chamaenerion angustifolium, Jul. 2004 , F.-Y. Bai (holotype CGMCC 2.2652 T preserved in a metabolically inactive state, ex-type CBS 15453 = XJ8A5).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.