Holtermannia saccardoi Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 99

Holtermannia saccardoi Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828784. Fig. 10K, L .

Etymology: the specific epithet saccardoi named in honour of P.A. Saccardo for his proposal of the genus Holtermannia .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are globosal, ovoid and ellipsoidal, 3.1–5.8 × 3.6– 6.4 μm and single, budding is polar ( Fig. 10K ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, mucoid, smooth and shiny. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal, 4.1– 5.9 × 7.4– 9.1 μm ( Fig. 10L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), sucrose, maltose, cellobiose, trehalose, lactose (variable), melibiose, raffinose, melezitose, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- Dglucoside, salicin, DL-lactate (variable), succinate (weak), citrate (variable) and myo-inositol are assimilated as sole carbon sources. L-sorbose, inulin, D-glucosamine, methanol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Sodium nitrite and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ho. saccardoi differs from its closely related species Ho. corniformis in its inability to assimilate L-sorbose and its ability to assimilate melibiose, raffinose, erythritol and potassium nitrate ( Table S1.13 ).

Typus: China, Simao county, Yunnan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3445 T preserved in a metabolically inactive state, ex-type CBS 15479 = SM37.10).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.