Kondoa rhododendri Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 107

Kondoa rhododendri Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828800. Fig. 14A, B .

Etymology: the specific epithet rhododendri refers to Rhododendron , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid, ellipsoidal and cylindrical, 2.7– 4.8 × 4.5– 9.5 μm and single, budding is polar ( Fig. 14A ), a sediment is formed. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pinkish cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are long ellipsoidal or ovoid, 3.0–4.3 × 7.9–10.0 μm ( Fig. 14B ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed), L-sorbose (delayed), sucrose, maltose, cellobiose (delayed and weak), trehalose, melezitose (delayed), inulin (weak), D-xylose (delayed), L-arabinose (delayed and weak), D-ribose (delayed and weak), ethanol (weak), glycerol (delayed), ribitol, galactitol (delayed and weak), D-mannitol and D-glucitol are assimilated as sole carbon sources. Lactose, melibiose, raffinose, soluble starch, D-arabinose, L-rhamnose, D-glucosamine, methanol, erythritol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine and ethylamine hydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 25 Ί C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. rhododendri differs well from other Kondoa species in its assimilation of carbon and nitrogen sources ( Table S1.17 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Rhododendron triflorum, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2763 T preserved in a metabolically inactive state, ex-type CBS 15457 = XZ27E3).

Figures

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.