Kondoa daliangziensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 105

Kondoa daliangziensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB832014. Fig. 12M, N .

Etymology: the specific epithet daliangziensis refers to the geographic origin of the type strain, Daliangzi River National Forest Park, Heilongjiang.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 2.7–4.4 × 4.3– 8.4 μm and single, budding is polar ( Fig. 12M ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale orange, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.6– 3.1 × 7.1– 8.6 μm ( Fig. 12N ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose (variable), maltose, cellobiose, trehalose, melezitose, L-arabinose (variable), ethanol (variable), glycerol, ribitol (variable), D-mannitol (variable), D-glucitol (variable), salicin (variable) and DL-lactate (variable) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, raffinose, inulin, soluble starch, D-xylose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, galactitol, Methyl-α- D-glucoside, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride (weak) and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 22–23 °C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. daliangziensis and Kon. ribitophobia are difficult to distinguish from each other. The latter can grow at 25 °C, but the former does not. Kon. daliangziensis differs from Kon. gutianensis in its inability to assimilate galactose and inulin and its ability to assimilate L-lysine ( Table S1.17 ).

Typus: China, Daliangzi river national forest park , Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2014, Q.-M. Wang (holotype CGMCC 2.5610 T preserved in a metabolically inactive state, ex-type CBS 13974 = HLJ22A8).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.