Gamasiphis angaridis, Marchenko, Irina I., 2013

Marchenko, Irina I., 2013, A new species of Gamasiphis Berlese (Acari: Ologamasidae) from North Asia, with a key to the Eurasian species, Zootaxa 3626 (3), pp. 381-390 : 382-387

Gamasiphis angaridis sp.n.

Diagnosis of adults (female and male). Anteromedial extension of epistome aciculate; all idiosomal setae aciculate; podonotal region of dorsal shield with 23 pairs of setae; opisthonotal region with 12 pairs of setae; seta j 2 about twice as long as j 1; seta j 4 about 0.9 times as long as distance between its base and base of j 5; seta z 6 about as long as j 6; seta s 6 about 0.9 times as long as z 6; seta j 6 about 0.7 times as long as distance between its base and base of J 2; four pairs of J setae; two pairs of pre-sternal shields; ventri-anal shields with eight pairs of setae in addition to para-anal setae (JV 1- JV 5, ZV 1- ZV 3); seta ZV 2 as long as distance between its base and base of ZV 3; seta JV 5 about 5–6 times as long as para-anal setae; setae JV 3 and JV 5 situated posterior to unsclerotised line that partially separates dorsal and ventri-anal shields; distance between ends of these lines shorter than or equal to distance between bases of JV 3 or these lines fully fused in some specimens.

Female. ( Figs 1–7 ) (35 specimens measured).

Gnathosoma: Fixed cheliceral digit 65–70 µm long with seven teeth in addition to apical tooth and a setiform pilus dentilis ( Fig. 1 ). Movable cheliceral digit 63–65µm long, with four teeth in addition to apical tooth. Dorsal cheliceral seta, lateral (antiaxial) and dorsal lyrifissures distinct. Epistome with anteromedian extension smooth and aciculate and with a pair of short anterolateral spines ( Figs 2 A–B). Deutosternal groove of hypostome with seven rows each bearing 7–14 denticles, except most basal row smooth ( Fig. 2 ); anterior-most row shaped as an inverted “V”, subsequent rows roughly transverse; margins of deutosternum not distinct. Setae h 1, h 2 and Sc (h 4) equal in length (32–35 µm), seta h 3 slightly longer than other (35–37 µm). The salivary styli well developed ( Fig. 3 ). Internal malae fimbriate laterally. Corniculi 38–40 µm long, 17–18 µm wide at the widest point ( Fig. 4 ). Palp chaetotaxy 2-5-6-14 -15; palp trochanter provided with one small ventral protuberance; setae al 1 and al 2 of palp genu slightly stout; palp apotele 3-tined.

Dorsal idiosoma: Dorsal shield entire, ovoid, smooth, totally covering dorsal surface, 425–480 µm long, 320–350 µm wide at level of coxa IV ( Fig. 5 ). Dorsal shield with 35 pairs of acicular setae. Podonotal region with 23 pairs of setae (j 1- j 6, z 1- z 6, s 1- s 6, r 2- r 6) and with nine pairs of distinguishable lyrifissures and three pairs of pores (posterior and slightly mediad to s 6, between r 3- r 4 and posterior to and mediad of r 6); with numerous sigilla posterior to j 5. Opisthonotal region with 12 pairs of setae (J 2- J 5, Z 2- Z 5, S 2- S 3, R 2- R 3) and with six pairs of distinguishable lyrifissures and two pairs of pores, including gdZ 2 especially pronounced (anterior and mediad to S 2) and one pair of pores on narrow diagonal sclerotised section of dorsal shield. Length of setae: j 1 (17–20), j 2 (40–45), j 3 (40–45), j 4 (45–50), j 5 (45–50), j 6 (45–50), z 1 (7–10), z 2 (7–10), z 3 (45–50), z 4 (45–50), z 5 (45–50), z 6 (45–50), s 1 (7–10), s 2 (7–10), s 3 (40–45), s 4 (60–65), s 5 (40–45), s 6 (40–45), r 2 (10–12), r 3 (10–12), r 4 (10–12), r 5 (25–30), r 6 (7–10), J 2 (7–10), J 3 (7–10), J 4 (7–10), J 5 (7–10), Z 2 (12–15), Z 3 (12–15), Z 4 (12–15), Z 5 (85–90), S 2 (12–15), S 3 (12–15), R 2 (12–15), R 3 (20–25).

Ventral idiosoma ( Fig. 6 ): Base of tritosternum 18–20 µm long and 20–22 µm wide proximally, laciniae (80–100 µm) totally separated from each other, pilose. Two pairs of pre-sternal shields present, slightly fused laterally. Sternal shield reticulate anteriorly between st 1 and st 2, smooth posteriorly; 70–75 µm long at mid-line and 135–140 µm wide between coxae II and III; with four pairs of setae (st 1, st 2, st 4 acicular; st 3 stout, inserted slightly anterior or slightly posterior or mesad to st 2) and four pairs of lyrifissures. Peritreme extending anteriorly to midlevel of coxa I. Peritrematic shield fused with section of exopodal shield near to coxa IV, widest at level of posterior margin of coxa IV, with a lyrifissure posterior to stigma. Length of peritrematic-exopodal shield from stigma to posterior margin 100 µm, width 50 µm at level of posterior margin of coxa IV. Band of dorsal shield extending laterad to the fused peritrematic-exopodal shield ending sharply in posterior margin. Genital shield wider than long, 80–90 µm long, 110–125 µm wide, abutting the sternal shield by hyaline apex; anterior margin convex and posterior margin truncate, with a pair of setae st 5 and three pairs of sigilla, distance between st 5- st 5 65–67 µm. Ventri-anal shield with transverse striations anteriorly to ZV 2 and smooth posteriorly; 230 µm long from anterior margin to post-anal seta, 250 µm wide at widest point, with eight pairs of acicular opishogastral setae (JV 1- JV5, ZV 1- ZV 3) in addition to post-anal and para-anal setae, and with four pairs of lyrifissures (anterior to and laterad of ZV 1, in transverse line and laterad of ZV 2, posterior to and mediad of ZV 3 and posterior to and laterad of para-anal seta). Post-anal seta about 5–6 times as long as para-anal setae, the latter usually situated at level of the posterior third or at the midlevel of anal opening. Dorsal and ventri-anal shields partly separated by an unsclerotised line; the distance between the ends of this line is shorter or equal to distance between bases of JV 3 or connected into a single fused line in some specimens; region posterior of this line 95 µm long; seta JV 3 posterior to this line. Narrow sclerotised diagonal section laterad of ventrianal shield that connects the latter to the dorsal shield with one pair of pores; 7–12 µm wide at the level of pore; ending sharply at anterior part. Length of ventral setae: st 1 (25–30), st 2 (25–30), st 3 (20–25), st 4 (20–25), st 5 (20–25), JV 1 (25–27), JV 2 (25–27), JV 3 (25–30), JV 4 (37–40), JV 5 (65–75), ZV 1 (25–30), ZV 2 (25–32), ZV 3 (25–32), para-anal (12–15) and post-anal seta (60–75).

Spermatheca: Opening part of spermathecal apparatus tubular, extending medially from base of coxa IV ( Fig.7 ). Other parts of spermatheca not clearly visible.

Legs: Lengths: I: 380–400, II: 237–288, III: 258–270, IV: 340–365 µm. Chaetotaxy of legs I–IV: coxa 2, 2, 2, 1; trochanter 6, 5, 5, 5; femur (2 3/2 2/2 2), (2 3/1 2/2 1), (1 2/1 2/0 0), (0 2/2 2/0 0); genu (2 3/2 3/1 2), (2 3/1 2/1 2), (2 2/1 2/0 1), (2 2/1 3/0 0); tibia (2 3/2 3/2 2), (2 2/1 2/1 2), (2 1/1 2/1 1), (2 1/1 3/1 1). All leg setae acicular, except one antero-lateral pilose seta on trochanter II. All legs with pretarsus, elongate ambulacral stalk, a pair of strongly sclerotised claws and rounded pulvillus.

Male. ( Figs 8–13 ) (24 specimens measured).

Gnathosoma: Fixed cheliceral digit 45 µm long, with five teeth in addition to apical tooth, with transverse line across the digit and a setiform pilus dentilis ( Fig. 8 A–B). Movable cheliceral digit 50 µm long, with one tooth in addition to apical tooth. Spermatodactyl 50–55 µm long, curved, widest at proximal part and gradually narrowing apically; with an internal canal along its entire length. Dorsal cheliceral seta, lateral (antiaxial) and dorsal lyrifissures distinct. Corniculi 37–38 µm long, 10 µm wide; with extension at inner side ( Fig. 9 ). Epistome and hypostome as in female. Setae h 1, h 2 and Sc equal length (30–33 µm), h 3 slightly longer than other (35–37 µm). Palps of male as in female ( Fig. 10 ).

Dorsal idiosoma: Dorsal shield 415–460 µm long, 275–330 µm wide, similar to that of female. Measurements of setae: j 1 (17–20), j 2 (35–40), j 3 (35–40), j 4 (35–40), j 5 (35–40), j 6 (35–40), z 1 (7–10), z 2 (7–10), z 3 (35–40), z 4 (35–45), z 5 (35–45), z 6 (35–45), s 1 (7–10), s 2 (7–10), s 3 (30–35), s 4 (50–55), s 5 (40–45), s 6 (35–40), r 2 (10–12), r 3 (10–12), r 4 (10–12), r 5 (25–30), r 6 (7–12), J 2 (7–10), J 3 (7–10), J 4 (7–10), J 5 (7–10), Z 2 (10–12), Z 3 (10–15), Z 4 (10–12), Z 5 (55–70), S 2 (12–15), S 3 (12–15), R 2 (12–15), R 3 (20–25).

Ventral idiosoma: Base of tritosternum 12–15 µm long and 20–22 µm wide proximally, with pilose lacinae (70–75 µm) ( Fig. 11 ). Except for the fusion of sternal and genital shields (sternitigenital shield), shape, pattern and fusions of ventral shields as in female. Sternitigenital shield reticulate between st 1 and st 2, smooth posteriorly; 117–120 µm long, 137–145 µm wide at widest point between coxae II and III; with five acicular setae (s t 1– st 5), distance st 1– st 1 55 µm, st 2– st 2 75 µm, st 3– st 3 90 µm, st 4– st 4 90 µm and st 5– st 5 90 µm; with four pairs of lyrifissures; genital opening on anterior margin of shield, flanked by two rounded flaps, with elongate pointed extensions. Ventri-anal shield 225–235 µm long from anterior margin to post-anal seta and 237–250 µm wide at widest point; with eight pairs of setae (JV 1– JV 5, ZV 1– ZV 3) in addition to post-anal and para-anal setae; and with four pairs of lyrifissures (anterior to and laterad of ZV 1, anterior to and laterad of ZV 2, posterior to and mediad of ZV 3 and posterior to and laterad of para-anal seta); post-anal seta about 4–5 times as long as para-anal seta. Length of ventral setae: st 1 (25–30), st 2 (25–30), st 3 (20–25), st 4 (20–25), st 5 (20–25), JV 1 (25–27), JV 2 (25–27), JV 3 (25–30), JV 4 (27–37), JV 5 (50–65), ZV 1 (25–30), ZV 2 (35–37), ZV 3 (20–25), para-anal (12–15) and post-anal seta (50–65).

Legs: Lengths: I: 400–437, II: 287–295, III: 263–285, IV: 360–368 µm. Chaetotaxy of legs similar to that of female. All setae of legs I, III, IV acicular ( Fig. 12 ). Legs II with one antero-lateral pilose seta on trochanter (similar to female); femur with two ventral spur-like setae: one large spur 40 µm length, in shape of twisted “long ear” and one small spur on elevated base; genu with two small ventral spur-like setae; tibia with one small ventral spur; tarsus with one small anterolateral spur near distal margin of basitarsus and with one acicular ventral seta on raised base ( Fig. 13 ). All legs with pretarsus, elongate ambulacral stalk, a pair of strongly sclerotised claws and rounded pulvillus similar to those of female.

Material examined. Holotype—female, Russia, South Siberia, North-Eastern Altai, Turochak district, environs of Kebezen village (51°55' N, 87°06' E), Pinus sylvestris—Abies sibirica — Betula pubescens forest, in litter, 22 June 2007, coll. I. Marchenko. Paratypes— 6 females on the same slide as holotype; 38 females, Altai, environs of Kebezen village, Pinus sylvestris forest, 11 August 2006, coll. I. Marchenko; 11 females, 2 males, Altai, environs of Obogo village (51°30' N, 87°18' E), Abies sibirica — Pinus sibirica forest, 6 August 2007, coll. I. Marchenko; 5 females, 2 males, Altai, environs of Saidyp village (52°34' N, 86°34' E), Betula pubescens — Populus tremula forest, 22 August 2006, coll. I. Marchenko; 2 females, 1 male; 2 females, 1 male, Middle Siberia, Krasnoyarskii Krai, environs of Turukhansk (65°47' N, 87°58' E), Picea sibirica—Pinus sibirica forest, 26 July 2003, coll. A. Babenko; 2 females, 1 male, Far East of Russia, Khabarovskii Krai, Grand Khekhtsir ridge (48°12' N, 134°52' E, 800 m a.s.l.), dark coniferous forest, 7 June 2011, coll. V. Dubatolov; 1 females, 6 males, Khabarovskii Krai, Nikolaevsk-na-Amure District, estuary of Amur River, environs of Arkhangelskoe village (53°05' N, 140°59' E), mixed forest with Quercus mongolica , 22 September 2009, coll. V. Dubatolov; 2 females, 3 males, Khabarovskii Krai, environs of Chegdomyn village (51°07' N, 132°58' E), Larix gmelinii forest, 18 July 2005, coll. I. Lyubechansky; 9 females, 2 males, Primorskii Krai, Sikhote-Alin Ridge (46°59' N, 136°53' E), June 2009, coll. D. Berman.

Holotype is deposited in Zoological Museum of Institute of Systematics and Ecology of Animals, Novosibirsk, Russia; 20 paratypes (17 females and 3 males) are kept in arthropods collection of Manchester Museum, Manchester, United Kingdom; other paratypes are deposited in Zoological Museum of Institute of Systematics and Ecology of Animals, Novosibirsk, Russia.

Other examined material: Russia, South Siberia: 240 females, 8 males, Altai, different districts (51°13'– 52°34’ N, 85°33' – 87°48' E); 5 females, Kemerovo Region, Mountain Shoria (53°20' N, 87°11' E); specimens were collected in litter and soil of South Siberian forests with Larix sibirica , Pinus sibirica , P. sylvestris , Abies sibirica , Picea abovata , Betula pubescens , Populus tremula , in the nests of Formica polyctena ; Far East of Russia: 4 females, Amur Region (55°19' N, 123°09' E); 9 females, Khabarovskii Krai, Komsomolsk-na-Amure District (50°29' N, 137°04' E); 6 females, 4 males, Nikolaevsk-na-Amure District, estuary of Amur River, environs of Arkhangelskoe village (53°05' N, 140°59' E); 28 females, 6 males, Grand Khekhtsir Ridge; 2 females, Primorskii Krai, Vladivostok city (43°10' N, 131°55' E); 26 females, Sikhote-Alin Ridge (46°59' N, 136°53' E); 76 females, 15 males, Ussuri State Reserve (43°48' N, 131°56' E); on mainland Far East of Russia specimens were collected in litter and soil of mixed forests with prevalence of Querqus mongolica and other mixed broad-leaved forests; 71 females, 15 males, Sakhalin Island (46°59'– 51°49' N, 142°51'– 143°09' E); Kuril Islands: 6 females, Kunashir (43°50’ N, 145°30' E); 2 males, Shikotan (43°48' N, 146°51' E); specimens were collected in litter and soil of island type forests with Betula ermanii , Quercus mongolica and bamboo Sasa spp.

Etymology. The name angaridis refers to the name of the paleo-continent Angarida which existed in the Paleozoic time and became the Siberian Platform of Eurasia in modern time.

Remarks. Females of G. angaridis are similar to those of G. novipulchellus Ma & Yin, 1998. However, the latter has 17 pairs of setae in the podonotal region and 10 pairs in the opisthonotal region of dorsal shield; seta s 6 is about 0.3 times as long as z 6; the narrow dorsal sclerotised diagonal section latered of ventri-anal shield is not indicated; exopodal shields between I and II coxae are not distinguishable; separating line at ventri-anal shield scarcely reaching bases of JV 4 setae; and the distance between bases of JV 4 and JV 3 setae is about 0.5 times as long as JV 4 seta. Males of G. angaridis are very similar to those of G. novipulchellus , but the latter has whip-shaped spermatodactyl, about twice as long as the movable digit of the chelicerae; and tarsus II lacks spur-setae.

Females of G. angaridis are similar also to those of G. aduncus Ma, 2004 . However the latter has 18 pairs of setae in the podonotal region and 10 pairs in the opisthonotal region of the dorsal shield; exopodal shields between coxae I and II are fused to the sternal shield; the separating line at ventri-anal shield scarcely reaches the bases of JV 3 setae; seta ZV 2 is about half as long as distance between its base and the base of ZV 3; seta JV 2 is about 0.3 times as long as distance between its base and base of JV 3. Males of G. angaridis are very similar to those G. aduncus , but the latter has a whip-shaped spermatodactyl about twice as long as the movable digit of the chelicerae; the basal part of spermatodactyl is narrow; femur II has a hook-shaped spur-seta; and tarsus II lacks a spur-seta.

Figures

FIGURES 1 – 7. Gamasiphis angaridis sp. n. Female. 1. Lateral (antiaxial) view of chelicera; 2. Epistome: A – B variations of shape; 3. Hypostome; 4. Corniculi; 5. Dorsal idiosoma; 6. Ventral idiosoma; 7. Spermatheca.

FIGURES 8 – 13. Gamasiphis angaridis sp. n. Male. 8. Lateral (antiaxial) view of chelicera: A – B; 9. Corniculi; 10. Palpa; 11. Ventral idiosoma; 12. Leg IV; 13. Leg II.