Lithotrichon pulchrum Darienko et Pröschold, 2017

Darienko, Tatyana & Pröschold, Thomas, 2017, Toward a monograph of non-marine Ulvophyceae using an integrative approach (Molecular phylogeny and systematics of terrestrial Ulvophyceae II.), Phytotaxa 324 (1), pp. 1-41 : 17

Lithotrichon pulchrum Darienko et Pröschold sp. nov. ( Fig. 9A–I )

Diagnosis: Plants in liquid 1/2 SWES medium up to 0.5 mm consist of prostrate and erected filaments. The system of prostrate filaments is very prominent and is formed from three-dimensional packages. The vegetative cells of the prostrate system are rounded or compressed from the sides, 5.9–8.8 μm in diameter, with a thick cell wall. Vegetative cells usually contain one or two large vacuoles. The erect system formed from short filaments consists of several cells (max. 10 cells). The end cells of plants are cylindrical, sometimes curved, 12.0–23.5 μm long × 3.5–5.5 μm wide and generally 2–4 times longer than broad. Branching is mostly bilateral. Cells are usually uninucleate, but sometimes the longest cells contain two nuclei and two pyrenoids (state before dividing?). Chloroplasts are parietal, usually filling the cell, with a pyrenoid. Zoosporangia are oval and approximately 16.2 μm in diameter, containing 8 zoospores. Zoospores are ovoid, 7.0–7.5 μm long × 4.0–4.5 μm wide with an anterior stigma. Zoospores are released by sudden fracture of the cell wall.

Habitat: Photobiont from lichen Verrucaria rheitrophila .

Type locality: Gladenbacher Bergland, near Dillenburg and Wetzlar, submerged approx. 10 cm below the water surface.

Holotype (designated herein): The authentic strain SAG 2038 is permanently cryopreserved in a metabolically inactive state (cryopreserved in liquid nitrogen) in the SAG, University of Göttingen , Germany.

Iconotype (designated herein in support of the holotype): Fig. 9 in this study.

ITS-2 DNA Barcode: LIT 1 in Figs S2.

Figures

FIGURE 9. Morphology of Lithotrichon pulchrum SAG 2038 grown on 1/2SWES medium. A, B. general view of plants, C–E. young plants, F–H. packets and short filaments, I. packets and settled zoospores.