Toxoplasma gondii

Ren, Hongjie, Yang, Liulu, Zhu, Niuping, Li, Junbao, Su, Chunlei, Jiang, Yibao & Yang, Yurong, 2022, Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains, International Journal for Parasitology: Parasites and Wildlife 19, pp. 330-335 : 331

2.3. Detection of antibodies against T. gondii

The heart fluids of the tigers were collected, double diluted from 1:25 to 1:12800, and tested for IgG antibodies against T. gondii using the MAT ( Dubey and Desmonts, 1987). Whole formalin fixed T. gondii antigens were obtained from the University of Tennessee Research Foundation (Knoxville, TN, USA). The tigers were exposed to T. gondii when the titre of antibodies against T. gondii is greater than 1:25. The reference serum was kindly provided by Dr. J. P. Dubey (ARS, USDA). The serum samples of the positive and negative controls were included in the same 96-well U plate.