Cryptoporus qinlingensis Y. Wang, X.C. Xie & Y. Song, 2024

Song, Yu, Wang, Yu-Yu, Wang, Yong & Xie, Xiu-Chao, 2024, Cryptoporus qinlingensis sp. nov., the third species of Cryptoporus (Polyporaceae, Polyporales) from northwestern China, Phytotaxa 672 (3), pp. 242-254 : 249-252

Cryptoporus qinlingensis Y. Wang, X.C. Xie & Y. Song sp. nov. Figs. 3 , 4

MycoBank:—MB854013

Diagnosis:— Cryptoporus qinlingensis Y. Wang, X.C. Xie & Y. Song sp. nov. differs from other species in the same genus by its relatively larger fruiting bodies (projecting up to 5.5 cm, 6.5 cm wide and 2.5 cm thick at base) with distinct odor of medicinal herbs when fresh, cream to reddish brown pileal surface, thick context (up to 1.5 cm thick), slim cystidioles (2.5–5.5 μm wide), pythogenesis on trunk of Pinus massoniana Lamb. and sequence data.

Etymology:—qinlingensis refers to Qinling mountains, a series of big mountains separating temperate and subtropical China, also the area where the specimens were collected.

Holotype:— CHINA. Shaanxi Province, Hanzhong City, Yang County, Qinling mountains , gregarious on trunk of dying but standing Pinus massoniana Lamb. , 30 March 2023, Y. Wang 23033001 ( SERC2417 ). Genbank nos: PP468343 (ITS), PP514383 ( LSU), PP461043 (mtSSU), PP588759 (rpb2), PP488943 (tef1), PP485896 (nSSU).

Description:— Basidiocarps annual, pileate, solitary, scattered or gregarious, with distinct odor of medicinal herbs when fresh, without taste, hard corky upon drying. Pilei ungulate when young, then oblate spheroidal, projecting up to 5.5 cm, 6.5 cm wide and 2.5 cm thick at base. Pileal surface eggshell color (5A3), pale brown with light reddish tint (5A5, 6A5), reddish brown (6B6, 5C8); margin extending over the pore surface as a volva with a small basal opening, color same to pileal surface, or paler (4A3, 5A3), sometimes light magneta (6C6). Pore surface cream (2A3) to buff (3A4), sometimes dark reddish brown (6C8) at margin; pores round, 1–2 × 0.5–1 cm; dissepiments thick, entire. Context whitish (1A1) to cream (1A2), soft leathery to soft corky, 4–15 mm thick. Tubes cream (2A2) to yellowish brown (4A4), obviously lighter than pore surface, 3–5 per mm, up to 5 mm long, hard corky.

Hyphal system trimitic; generative hyphae bearing clamp connections; skeletal and binding hyphae negative in Melzer’ reagent, positive in Cotton Blue; tissues darkening in KOH. In Context, generative hyphae infrequent, hyaline, thin-walled, occasionally branched, 2–3 μm in diam; skeletal hyphae dominant, hyaline, thick-walled to subsolid, branched, interwoven, 2.5–4 μm in diam; binding hyphae abundant, thick-walled, frequently branched, strongly interwoven, 1.5–3.5 lm in diam. In Tubes, generative hyphae infrequent, hyaline, thin-walled, occasionally branched, 1.8–2.8 μm in diam; skeletal hyphae dominant, hyaline to pale yellowish brown, thick-walled to subsolid, frequently branched, interwoven, 2.5–4.5 μm in diam; binding hyphae abundant, frequently branched, thick-walled, strongly interwoven, 1.3–3.5 μm in diam. Cystidia absent; fusoid cystidioles present, thin-walled, smooth, 12.5–19–26.5 × 2.5–4–5.5 μm. Basidia clavate, 4-spored, with a basal clamp connection, 14.5–18-24 × 5–6-8 μm; sterigmata up to 4 μm; basidioles in shape similar to basidia, but smaller. Basidiospores cylindrical to oblong-ellipsoid, hyaline, thick-walled, smooth, (8–)8.4–9.82–11.3(–11.7) × (3–)3.7–4.43–5.3(–5.6) μm, Q = (1.72–)1.85–2.25–2.98(–3.45) (n=60/3/3), negative in Melzer’ reagent, positive in Cotton Blue.

Habitats:—only found on trunk of Pinus massoniana Lamb. in Qinling mountains located in Shaanxi Province, northwestern China.

Additional specimen examined:— CHINA. Shaanxi Province, Hanzhong City, Yang County, Qinling mountains , scattered on trunk of fallen Pinus massoniana Lamb. , 16 April 2022, Y. Song 22041608 ( SERC2418 ), Genbank nos: PP468344 (ITS), PP514384 ( LSU), PP461044 (mtSSU), PP588760 (rpb2), PP488944 (tef1), PP485897 (nSSU) ; Same data as for preceding, solitary on trunk of standing Pinus massoniana Lamb. , 23 March 2024, Y. Song 24032301( SERC2425 ), Genbank no: PP758830 (ITS) ; Same data as for preceding, scattered on trunk of fallen Pinus massoniana Lamb. , 23 March 2024, X.C. Xie 24032001 ( SERC2426 ), Genbank no: PP758831 (ITS) .

Notes:— Cryptoporus volvatus (Peck) Shear , the type species of the genus, can be separated from the novel species by its relatively smaller basidiocarps (projecting up to 4.5 cm, 3.5 cm wide and 2.5 cm thick at base) with cream to deep pinkish buff pileal surface and very thin context (1-2 mm thick), larger basidia (17–22 × 7–10 μm) and spores (10.44 × 4.74 μm in average), wider cystidioles (18–24 × 7–9 μm) ( Cui et al. 2019).

Cryptoporus sinensis Sheng H. Wu & M. Zang recorded in central and southern China, is the second described species of the genus. Cryptoporus sinensis is closely related to the novel species in macromorphology, but it differs from the latter by its relatively smaller basidiocarps (projecting up to 2 cm, 3 cm wide and 1 cm thick at base) with cream to pinkish buff pileal surface and spores (8.92 × 3.95 μm in average), and thin context (less than 7 mm thick). In addition, C. sinensis is associated with Pinus khasya Royle et Gord and Pinus densata Masters ( Wu & Zang 2000, Cui et al. 2019), while the novel species is only found on trunk of Pinus massoniana Lamb .. The fresh basidiocarps of C. qinlingensis sp. nov. has distinct odor of medicinal herbs, which is also different from other related species without odor.

Figures

FIGURE 3. Fruiting bodies of Cryptoporus qinlingensis sp. nov. A–D. SERC2417 (holotype). E. SERC2418. F. SERC2425. G. SERC2426. Scale bars = 1 cm.

FIGURE 4.Microscopicstructures ofCryptoporus qinlingensis sp.nov.(SERC2417,holotype).A.Basidia.B. Basidioles.C. Basidiospores. D. Cystidioles. E. Hyphae from trama. F. Hyphae from context. Scale bars: A–D = 5 μm, E–F = 10 μm.