Crepidotus iqbalii A. Izhar, Usman & Khalid, 2021

Izhar, Aiman, Usman, Muhammad & Khalid, Abdul Nasir, 2021, Crepidotus iqbalii (Crepidotaceae, Agaricales) a new stipitate species, from Pakistan, Phytotaxa 500 (2), pp. 95-107 : 100-103

Crepidotus iqbalii A. Izhar, Usman & Khalid sp. nov. Figs. 2–4

MycoBank:—MB838246

Holotype:— PAKISTAN. Punjab, Sheikhupura , on muddy soil of graveyard, (31°43’3.71”N, 73°57’19.73”E), 236 m a.s.l., 18 August 2018, Aiman Izhar, Skp003 ( LAH 36654).

Diagnosis:—Pileus brown to yellow, hygrophanous. Stipe central to slightly eccentric. Basidiospores 8.7–10 × 4.9–6.3 μm, ellipsoid-amygdaliform, almost smooth to finely verruculose. Cheilocystidia clavate to subcylindric. Pleurocystidia absent. Both pileipellis and the stipitipellis a trichoderm with many cystidia. Clamp connections present.

Etymology:—In honor of Prof. Dr. S. H. Iqbal (Late) Mycologist, for his contributions to fungal taxonomy in Pakistan.

Gene sequence ex-holotype:—GenBank MT 97348 ( ITS) and MW 888515 ( LSU).

Description:— Pileus 15–30 mm diam, reniform when seen from above, hemispherical to convex, a few almost circular, some later becoming flabelliform, bright brown (7.5 YR 5/6) when immature, pale yellow (5 Y 8/4) to yellow (10 YR 8/8) at maturity, umbilicate at disc with dull brown color (7.5 YR 5/4), flabby, hygrophanous, surface smooth to velvety, minutely tomentose near disc, margin straight, later inflexed, tuberculate-striate to rimose. Lamellae 2–4 mm broad, adnate, some emarginate with a decurrent tooth, yellow (10 YR 8/8) when young, later fading to pale yellow (5 Y 8/4), moderately crowded, furcate, edges entire to undulate. Stipe 10–17 × 3–6 mm, central to slightly eccentric, cylindrical, forked near the cap, base broad, pale yellow (5 Y 8/4) near pileus, white to dull orange (7.6 YR 6/4) towards base, turning dark brown (10 YR 3/4) on handling, rubbery, hollow, minutely pruinose, mycelial pad at base. Annulus absent. Odor not characteristic. Taste not checked.

Basidiospores 8.7–10 × 4.9–6.3 μm, Lm × Wm = 9.6 ± 0.7 × 5.4 ± 0.4 μm, Q = 1.7–1.6 μm, avQ = 1.8, ellipsoidamygdaliform, almost smooth to very finely verruculose, thick-walled, with a large and many little brownish gray (10 YR 6/1) guttules. Basidia 22–34 × 7–9 μm, cylindrical to clavate, clamped, mostly 4-spored, few 2-spored, hyaline, thin-walled, granulate, a few large oil drops present. Cheilocystidia 14–28 × 3.6–6.8 μm, mostly clavate or flexuous less cylindric, mostly often septate, clamped. Pleurocystidia absent. Pileipellis a trichoderm, with hyaline, cylindrical hyphae, 4–8 μm, avw = 6 μm, septate, branched, gelatinization present in some of the hyphae, frequently clamped, terminal cells cylindrical or clavate (5–9 μm), occasionally branched. Pileocystidia 19.8–38 × 6.3–7.8 μm, clavate to cylindrical, at times narrowly clavate, lageniform, narrowly utriform, mostly flexuous, clamped. Stipitipellis an intricate trichoderm, with interwoven hyphae 7–5 μm (avw = 4.1 μm), thin to slightly thick-walled, hyaline, septate, some hyphae with gelatinization terminal cells cylindric, clamped. Caulocystidia 10–17 × 3.8–7.6 μm, mostly lageniform to utriform, few lecythiform, clavate, flexuous, grouped, basal cells slightly inflated, hyaline, clamped.

Habitat and distribution:—Pleurotoid, solitary, on muddy soil of a burial ground which adjoins a church, under Ficus benghalensis .

Additional material examined:— PAKISTAN. Punjab, Sheikhupura , on muddy nutrient rich soil of graveyard, (31°43’3.71”N, 73°57’19.73”E), 236 m a.s.l., 4 August 2019, Aiman Izhar, Skp1b ( LAH 36655), GenBank MT 973499 ( ITS) and MZ 081842 ( LSU) ; ibid. (31°42′40″ N, 73°59′16″ E, 236 m a.s.l., 6 September 2020, Aiman Izhar, Skp2 B ( LAH 36656).

Figures

FIGURE 2. Basidiomata of Crepidotus iqbalii, A, B = SKP003 (Holotype), C, D = SKP1b, E = SKP2b, Scale Bars A–E = 1 cm. Photos by:Aiman Izhar.

FIGURE 3. Micromorphological characters of Crepidotus iqbalii. A–H (SKP003, Holotype): A-C = Basidia, D = Cheilocystidia, E = Basidiospores, F = Pileipellis hyphae, G = Pileocystidia, H = Stipitipellis hyphae. Scale Bars A–H = 10 μm. Photos by: Aiman Izhar

FIGURE 4. Micromorphological characters of Crepidotus iqbalii. A–G (SKP003, Holotype): A = Basidia, B = Basidiospores, C = Cheilocystidia, D = Pileocystidia, E = Caulocystidia, F = Pileipellis hyphae, G = Stipitipellis hyphae. Scale Bars A–G = 10 μm, Drawings by Aiman Izhar.