Neogreenia lonicera Wu & Nan, 2012

Zheng, Xinyi, Watson, Gillian W., Zhang, Jiangtao, Tan, Zhixiang & Wu, San’An, 2024, A review of the genus Neogreenia MacGillivray (Hemiptera: Coccomorpha: Qinococcidae) with descriptions of two new species, Zootaxa 5418 (5), pp. 401-441 : 410-412

Neogreenia lonicera Wu & Nan, 2012

Neogreenia lonicera Wu & Nan 2012: 47 . Type data: CHINA: Inner Mongolia, Helan Mountain, Bayan Hot, under the bark of Lonicera microphylla. Holotype, female, by monotypy and original designation. Type depository: Beijing Forestry University, Beijing, China.

Host plants: Lonicera microphylla Willd. ex Roem. et Schult. ( Caprifoliaceae ).

Distribution: China (Inner Mongolia).

Remarks. Wu & Nan (2012) described and illustrated the adult female, first-instar nymph and second-instar nymph. After examining original material of cysts of N. lonicera , we noticed that the cyst stage described by Wu & Nan (2012) was not a “second-instar nymph” but a third-instar female nymph, since their specimens had the anterior 6 abdominal spiracles each with sieve-like disc-pores in the atria (a diagnostic character for third-instar female nymphs). The second-instar nymph of N. lonicera is therefore described below.

Material examined. Paratypes: 2 second-instar nymphs, CHINA: collection data same as for holotype, mounted together with 2 third-instar ♀♀ nymphs .

Second-instar nymph (cyst) (sexes indistinguishable)

Slide-mounted material (n=2) ( Fig. 5 ). Body oval, 1.2–1.6 mm long and about 0.8 mm wide; derm membranous but posterior apex slightly sclerotized. Antennae ( Fig. 5A ) situated close together medially on head; each reduced, plate-like, 15–18 μm in diameter, with margin sclerotized and protruding anteriorly, and bearing 5–7 setae, each seta 10–20 μm long. Eyes absent. Labium ( Fig. 5B ) 2 segmented, about 100 μm long and 80–83 μm wide; segment I with a pair of short setae (each about 5 μm long) on each side; segment II with 2 apical hair-like setae (each about 30 μm long) on each side. Clypeolabral shield longer than labium, 230–250 μm long and 125–130 μm wide; stylets present. Legs absent. Thoracic spiracles ( Fig. 5C ) sclerotized, each with opening 10–13 μm in diameter, a sclerotized bar and a group of 4 or 5 sieve-like disc-pores ( Fig. 5D ) at inner end of atrium. Abdominal spiracles numbering 8 pairs, with anterior 5 pairs sclerotized; anteriormost pair ( Fig. 5E ) each with opening about 10 μm in diameter, and 2 sieve-like disc-pores within atrium; spiracles ( Fig. 5F ) on abdominal segments II– V smaller, each with opening about 7.5 μm in diameter, and 1 sieve-like disc-pore within atrium; posteriormost 3 pairs unsclerotized and tube-like, each with opening about 2.5 μm in diameter and without pores in atrium. Anal opening 12–15 μm wide, with U-shaped sclerotized ring lacking pores or setae, located medially on posteriormost dorsal segment. Cicatrices absent.

Dorsum. With disc-pores of 2 types: (i) large simple pores ( Fig. 5G ), each about 10 µm in diameter; usually 1 pore present submarginally on each segment, but some segments with 2 pores or none; and (ii) compound multilocular disc-pores ( Fig. 5H ), each about 7.5 µm in diameter, with 4–7 (mostly 5 or 6) subcentral loculi and distinct peripheral loculi, present on margins from head to abdominal segment II. Setae ( Fig. 5I ) of only 1 type, spine-like, each 5–10 μm long, present in submarginal and submedial areas.

Venter. With disc-pores of 2 main types: (i) compound multilocular disc-pores ( Fig. 5H ), same size and structure as on dorsum; forming group around each thoracic spiracle and present on margins except for 2 posteriormost segments; also smaller ones ( Fig. 5J ), each about 5 µm in diameter, with 3–5 subcentral loculi but without peripheral loculi, numbering 2 or 3 between antennae; and (ii) sieve-like disc-pores ( Fig. 5D ), each 5–7 µm in diameter, with many irregularly distributed loculi, present within atria of thoracic spiracles and anterior 5 pairs of abdominal spiracles. Setae of 3 types: (i) hair-like setae, each 13–18 μm long, situated on prothorax submedially, below mesothoracic spiracles and submarginally on abdominal segment I; (ii) spine-like setae ( Fig. 5I ), same size as on dorsum, few on head margin; and (iii) short conical spine-like setae ( Fig. 5K ) with sclerotized basal sockets, each about 5 μm long; with 1 or 2 near each hair-like seta, 2 or 3 near each thoracic spiracle, and 0 or 1 near each abdominal spiracle except for posteriormost pair.

Figures

FIGURE 5. Second-instar nymph of Neogreenia lonicera Wu & Nan: A.Antenna; B. Labium; C. Thoracic spiracle; D. Sieve-like disc-pore; E. Anteriormost abdominal spiracle; F. Abdominal spiracles on segments II–V; G. Large simple pore; H. Compound multilocular disc-pores; I. Spine-like seta; J. Compound multilocular disc-pores between antennae; K. Short conical spine-like seta with sclerotized basal socket.