Entoloma kumraticus A. Izhar, Kiran, Usman & Khalid

Izhar, Aiman, Usman, Muhammad, Kiran, Munazza & Khalid, Abdul Nasir, 2022, Two new species of Entoloma (Entolomataceae) from Khyber Pakhtunkhwa, Pakistan, Phytotaxa 574 (3), pp. 226-240 : 231-233

Entoloma kumraticus A. Izhar, Kiran, Usman & Khalid sp. nov. ( Figs. 2 & 3 )

MycoBank:—MB843782

Etymology:—The specific epithet kumraticus refers to the type locality “Kumrat”.

Diagnosis:— Entoloma kumraticus closely resembles E. porphyrogriseum described from Austria but differs by having white to pallid purplish gray lamellae, significantly smaller cheilocystidia and the presence of caulocystidia.

“ Type:— PAKISTAN. Khyber Pakhtunkhwa Province: Dir Upper District, Kumrat , among grass, on soil under Cedrus deodara , 35.5369˚ N, 72.2193˚ E, 2232 m, 25 July 2019, A. N. Khalid K -218, ( LAH 36945!; ITS: MZ 157265 ; LSU: MZ 157269 ).”

Description:— Pileus 1.8–2.5 cm in diam., hemispherical, convex to plano-convex, deeply depressed at disc, deflexed towards margin, margin entire to sulcate-striate, dark aniline blue (X55) at center changing to prout`s brown (XV15’) to mummy brown (XV17’) towards margin, pileal surface radially fibrillose, dry, velutinous, shiny when moist, slightly hygrophanous. Lamellae adnate, slightly adnexed, sub-distant, white (LIII) to pallid purplish gray (LIII 67’””), regular, some crisped, edges serrulate, lamellulae abundant, present in 2–3 tiers. Stipe 3.2–5 × 0.3–0.6 cm, cylindrical with slightly tapered base, hollow, pale neutral gray (LIII d) to purplish gray (LIII 67””) at apex, pallid bluish violet (X 57f) towards base, fibrillose, minutely pubescent all over, dry, some with white (LIII) tomentose base. Odor mild.

Basidiospores [40/2/2], (8–)9–11(–12) × (6.1–)6.4–8 µm, (avL × avW= 9.7 × 7.3 µm), Q = 1.25–1.38, avQ = 1.32, heterodiametrical with 4–6 weak angles, thin-walled, olive yellow (XXX 23”) in KOH, inamyloid, mono-guttulate. Basidia (29–)33–44(47–) × (9–) 10–11(12) µm, (avL × avW = 38 × 10.5 µm), broadly clavate, hyaline in KOH, mostly 4-spored, occasionally 2-spored, guttulate, clamp connections absent. Lamella edge sterile, cheilocystidia abundant, 12–34 × 4.9–8.4 µm, cylindroclavate, some flexuous, thin-walled, hyaline in KOH, with 2-3 basal cells, nonguttulate. Hymenophoral trama regular, composed of cylindrical hyphae with inflated cells, 80 – 125 × 4 – 9 µm, brilliant granules abundant. Pileipellis a cutis of loose cylindrical hyphae, in transition to a trichoderm towards the pileal center where the hyphae are repent, with inflated, clavate terminal elements, 30–93 × 15–25 µm, cells of suprapellis 22–80 × 9–11 µm, a subpellis of relatively narrow, septate, cylindrical hyphae, 3–7 µm wide, (avW = 5 µm), pigments intracellular in terminal cells, grayish brown in water, relatively dark brown in KOH, clamp connections absent. Stipitipellis a cutis of cylindrical hyphae, with cylindrical to clavate terminal elements, 19–33 × 1.6–5.8 µm, mostly hyaline in KOH, some terminal elements with dark brown brilliant granules, strongly refracting, hyphae regular, septate, rarely branched, clamp connections absent. Caulocystidia clavate to narrowly clavate, versiform, 24 – 64 × 7 – 15 μm, scattered.

Additional material examined: PAKISTAN. Khyber Pakhtunkhwa Province: Dir Upper District, Kumrat , mostly solitary on moss covered soil under Cedrus deodara , 35.5369˚ N, 72.2193˚ E, 2232 m, 15 August 2018, M. Usman & A. N. Khalid K -219, ( LAH 36946!; ITS: MZ 157266 ; LSU: MZ 157270 ) .

Figures

FIGURE 2. A–C: Basidiomata of Entoloma kumraticus; D: Pileipellis, E: Basidiospores; Scale Bars A–C = 1 cm, D = 20 µm, E = 10 µm. Microscopic photos by: Aiman Izhar.

FIGURE 3. A–E. Entoloma kumraticus (Holotype). Elements of hymenium drawn as shown by light microscopy A: Basidia, B: Basidiospores; C: Cheilocystidia; D: Pileipellis; E: Stipitipellis with caulocystidia; Scale Bars A–E = 10 µm. Drawings by: Aiman Izhar.