Isoperla riverae, Verdone & Beaty & Holland & Williams, 2023

Verdone, Chris J., Beaty, Steven R., Holland, Victor B. & Williams, Bronwyn W., 2023, Isoperla riverae, a new stonefly species from the southeast Nearctic, with notes on sympatric species including the larval description of Isoperla lenati Szczytko & Kondratieff, 2015 (Plecoptera: Perlodidae), Zootaxa 5270 (3), pp. 437-470 : 446-447

Isoperla riverae sp. n. Verdone, Beaty, Holland & Williams

( Figs. 16–39 )

Isoperla dicala: Stark & Gaufin, 1978:401

Isoperla dicala: Szczytko & Kondratieff, 2015:88 (in part)

Etymology. The new species is named for the senior author’s daughter. May she enjoy the splendor of the natural world and value all its inhabitants. The proposed common name is “River’s Stripetail”.

Distribution. U.S.A. —AL, FL, GA, NC, MS, SC, TN, VA ( Fig. 15 )

Material examined. Holotype M: U.S.A. — North Carolina: Moore / Richmond Co., Drowning Creek , SR 1102, 35.08426, -79.58270, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty ( NMNH) . Paratypes: Georgia: DeKalb Co., Snapfinger Creek , 5 mi SE of Panthersville, [33.66552, -84.21116], 4 June 1945, P.W. Fattig, 9M ( INHS, Insect Collection 875867) . Gwinnett Co., Ivy Creek , 5 mi NS Buford, [34.04788, -84.03133], 11 June 1945, P.W. Fattig, 2M ( INHS, Insect Collection 875931) . North Carolina: Caldwell Co., Johns River, Old Johns River Rd. , [35.93359, -81.69053], 17 May 2004, B. Kondratieff, D. Lenat, R. F. Kirchner, R. Zuellig, 1M ( NCDWR) ; same location, 20 May 2019, C. Verdone, S. Beaty, V. Holland, 1M ( NCDWR) . Henderson Co., French Broad River, Hwy 64, [35.32853, -82.57524], 23 May 1990, B. Kondratieff, R. F. Kirchner, 5M, 3F ( NMNH) . Moore Co., Drowning Creek , SR 1004, 35.06575, -79.54998, 28 April 2022, emerged 4 May 2022, C. Verdone, S. Beaty, V. Holland, 1M ( NCDWR) . Moore / Richmond Co., Drowning Creek , SR 1102, 35.08426, -79.5827, 19 May 2018, C. Verdone, 2M, 61F ( NCDWR) ; same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 1M, 1E ( NCDWR) ; same location, 28 April 2019, emerged 6 May 2019, C. Verdone, S. Beaty, 2M ( NCDWR) ; same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1M, 1E ( NCDWR) . same location, 28 April 2019, emerged 13 May 2019, C. Verdone, S. Beaty, 1F ( NCDWR) . Richmond Co., Naked Creek , SR 1003, 35.08197, -79.58909, 27 April 2012, emerged 30 April 2012, D. Lenat, 1F,1E ( NCDWR) ; same location, 27 April 2012, emerged 5 May 2012, D. Lenat, 2F, 2E ( NCDWR) ; same location, 28 April 2019, C. Verdone. S. Beaty, 1F, 1E, 3L ( NCDWR) ; same location, 30 April 2019, C. Verdone, S. Beaty, 1F, 1E ( NCDWR) ; same location, 9 May 2020, C. Verdone, V. Holland, 1M, 1F ( NCDWR) . Rockingham Co., Mayo River , SR 1358, 36.53483, -79.99086, 11 May 2018, C. Verdone, S. Beaty, V. Holland, 1M ( NCDWR) ; same location, 15 May 2018, C. Verdone, S. Beaty, V. Holland, L. Housley, 2M ( NCDWR) ; same location, 21 May 2019, V. Holland, 1M ( NCDWR) . South Carolina: Aiken Co., Upper Three Runs, Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B. Kondratieff, 29M, 8F ( NCDWR) . Virginia: Franklin Co., Big Chestnut Creek , Rte 718 E, [36.87630, -79.86254], 21 May 2002, B. Kondratieff, R. F. Kirchner, 2M ( NMNH) .

Additional material examined. U.S.A. — Alabama: Clarke Co., Fishers Creek, nr Whatley , [31.63982, - 87.71937], 8 May 1986, S. Harris, P. O’Neil, 44M, 11F ( NCDWR) . Cleburne Co., Shoal Creek, Shoal Creek Campground, [33.77071, -85.55572], 25 May 1984, S. Harris, P. Lago, 4M, 2F ( NCDWR) ; South Fork Terrapin Creek, Hwy 55, [33.86017, -85.52440], 25 May 1984, S. Harris, P. Lago, 3M, 1F ( NCDWR) . Monroe Co., Little River, Hwy 59, [31.29888, -87.71048], 11 May 1982, S. Harris, 1M ( NCDWR) . Randolph Co., Cutnose Creek, Hwy 48, [33.42541, -85.37436], 20 May 1984, S. Harris, P. Lago, 1M, 2F ( NCDWR) . Tallapoosa Co., Josie Leg Creek, off Hwy 22, [32.98634, -85.85926], 19 June 1983, S. Harris, P. Lago, 1M ( NCDWR) . Florida: Escambia Co., Canoe Creek, above Bratt Rd. , [30.95664, -87.34758], 24 April 2009, D. Ray, 1M ( NCDWR) . Walton Co., Alaqua Creek, Base Rd. 201, Eglin Air Force Base , [30.61667, -86.16444], 17 April 2006, R. W. Flowers, M.L. Pescador, A.K. Rasmussen, B.A. Richard, 10M ( NCDWR) . Georgia: Bartow Co., Clear Creek , 6 mi N Cartersville, [34.27560, -84.87250], 3 June 1939, P.W. Fattig, 1M ( INHS, Insect collection 875885) ; Pettit Creek , 2 mi N Cartersville, [34.22127, -84.80251], 3 June 1939, P.W. Fattig, 2F ( INHS, Insect Collection 875855) . Fulton Co., Nancy Creek , 8 mi N Atlanta, [33.86331, -84.40475], 26 June 1945, P.W. Fattig, 3F ( INHS, Insect Collection 875853) . Hall Co., no waterbody data, Gainesville , [34.29778, -83.82417], 9 May 1944, T. H. Frison, H.H. Ross, 1F ( INHS, Insect Collection 875862) . Screven Co., Brier Creek , 7 mi NE Sylvania, [32.83172, -81.56612], D.C. Scott, 1M ( INHS, Insect Collection 875895) . Union Co., Wolf Creek, 3.5 mi N Neel [s] Gap , [34.78515, -83.90730], 30 May 1943, P.W. Fattig, 3M ( INHS, Insect Collection 875891) ; same location, 8 June 1945, P.W. Fattig, 5M ( INHS, Insect Collection 875875) . Mississippi: Clarke Co., Moore Mill Creek, Clarkco State Park , [32.10818, -88.69334], 26 May 1990, Tanner, Parham, 1F ( NCDWR) . Lamar Co., Sandy Run, Okahola Rd. , [31.20737, -89.38775], 13 May 1992, B. Stark, M. Hicks, 2F ( NCDWR) . Pike Co., Tangipahoa River , 3 mi Magnolia, [31.11275, -90.47943], 25 March 1977, B. Stark, 2M, 1F ( NCDWR) . Simpson Co., Mill Creek, Bushtowns Shiver Rd. , [31.84672, - 89.98869], 12 May 1992, B. Stark, E. Stark, C. Bishop, 1M, 5F ( NCDWR) ; Mill Creek, Hwy 472, [31.86365, - 89.99195], 3 May 1990, B. Stark, 2M, 5F ( NCDWR) ; Strong River, 3 mi W of Pinola, Hwy 28, [31.88622, - 89.99349], 22 June 1988, S. Szczytko, 1M ( NCDWR) ; Westville Creek, Jake Barnes Rd. blw. Hale Branch jct., [31.87234, -89.91891], 13 April 1991, B. Stark, 6M, 3F ( NCDWR) . Smith Co., Clear Creek , 3 mi SW Mize [31.84949, -89.59955], 24 April 1991, B. Stark, D. Tanner, 12F ( NCDWR) . North Carolina: Alleghany Co., Glade Creek , SR 1422, nr mouth, 36.50000, -81.03639, 20 June 2008, S. Beaty 15L ( NCDWR) . Ashe Co., Cranberry Creek, SR 1609, 36.4028, -81.29833, 13 May 1998, D. Lenat, 1L ( NCDWR) ; Peak Creek, Off SR 1599, 350M upstream Ore Knob Branch , 36.42083, -81.31944, 15 April 1991, D. Penrose, 1L ( NCDWR) ; Cranberry Creek , SR 1609, 36.44028, -81.29833, 13 May 1998. D. Lenat, 1L ( NCDWR) . Buncombe Co., South Hominy Cr, Davis Creek Rd. , 35.47831, -82.75003, 2 June 2010, V. Holland, 1L ( NCDWR) ; South Hominy Creek, Dale Pen Rd. , 35.48817, -82.74259, 1 June 2010, V. Holland, 1L ( NCDWR) ; [Swannanoa River], Swannanoa , 35.59789, - 82.39984], 19 June 1938, T. H. Frison, T. H. Frison Jr., 2M ( INHS, Insect Collection 875897) ; same data, 2M ( INHS, Insect Collection 875851) . Burke Co., Black Fox Cr, SR 1917, 35.66417, -81.63278. 19 April 1989, T. McPherson, 15L ( NCDWR) ; Henry Fork , NC 18 , 35.67361, -81.53611, 20 April 1988, D. Lenat, 25L ( NCDWR) ; Henry Fork, SR 1803, 35.68944, -81.45028, 1 May 2006, B. Crouch, 15L ( NCDWR) ; Henry Fork SR 1854, 35.69556, -81.42722, 1 May 2006, B. Crouch, 16L ( NCDWR) ; Henry Fork, SR 1916, 35.66167, -81.60361, 2 May 2006, B. Crouch, 10L ( NCDWR) ; Henry Fork, SR 1922, 35.66167, -81.63694, 19 April 1988, T. McPherson, 15L ( NCDWR) ; same location, 2 May 2006, T. Mormon, 11L ( NCDWR) ; Ivy Creek , SR 1919, 35.64111, -81.66167, 19 April 1988, D. Lenat, 1L ( NCDWR) ; Jacob Fork, SR 1924, 35.59056, -81.56722, 2 May 2006, B. Crouch, 6L ( NCDWR) ; Little Fork , FSR 228, 35.91056, -81.81833, 19 May 2015, M. Walters, 1L ( NCDWR) ; Rock Creek , SR 1915, 35.64167, -81.55750, 19 April 1988, D. Lenat, 2L ( NCDWR) ; Steels Creek , at FR 228, [35.912264, -81.838327] 25 June 1988, S. Dunkle, 1M ( NCDWR) ; Upper Creek, FSR 982 upstream of Timbered Br , 35.92000, -81.79306, 13 June 1994, D. Penrose, 1L ( NCDWR) . Caldwell Co., Abingdon Creek, SR 1315, 35.91344, -81.60104, 18 April 2011, T. Mormon, 1L ( NCDWR) ; Yadkin River, off SR 1372 downstream of Jackson Camp Creek , 36.06895, -81.58553. 23 May 2011, D. Black, E. Fleek, S. Beaty, 2L ( NCDWR) . Catawba Co., Henry Fork, SR 1124, 35.68417, -81.40278. 1 May 2006, B. Crouch, 16L ( NCDWR) . Cleveland Co., Brushy Creek, SR 1323, 35.33972, -81.61580, 16 May 1995, N. Guthrie, 1L ( NCDWR) . Davidson Co., Abbott Creek , SR 1735, 36.00833, -80.0775, 10 May 2006, D. Black, S. Beaty, B. Prusha, 4L ( NCDWR) ; Brushy Fork. SR 1810, 35.92444, -80.18028, 14 May 2003, T. Mormon, 9L ( NCDWR) . Franklin Co., Sandy Creek , US 401, 36.24806, -78.25833, 3 May 1988, T. McPherson, 3L ( NCDWR) . Gaston Co., Beaverdam Creek , SR 1609, 35.40444, -81.24528, 5 May 2006, B. Crouch, B. Prusha, M. Walters, 15L ( NCDWR) ; Hoyle Creek , SR 1836, 35.33472, -81.13389, 4 May 2006, B. Crouch, 2L ( NCDWR) ; Long Creek , SR 1405, 35.30194, -81.31333, 4 April 1994, N. Guthrie, 1L ( NCDWR) ; same location, 18 April 1995, D. Penrose, 1L ( NCDWR) ; Long Creek , NC 275 , 35.30222, -81.22278, 5 April 1991, L. Eaton, 1L ( NCDWR) ; same location, 1 April 1992, D. Lenat, 1L ( NCDWR) ; same location, 19 April 1995, D. Penrose, 1L ( NCDWR) ; Long Creek , NC 274 , 35.30778, -81.30333, 4 April 1994, D. Penrose, 1L ( NCDWR) ; same location, 18 April 1995, D. Penrose, 2L ( NCDWR) ; Long Creek , SR 1448, 35.31222, -81.26472, 4 April 1994, K. Herring, 1L ( NCDWR) , Long Creek , SR 1446, 35.31639, -81.27194, 5 April 1994, D. Penrose, 2L, ( NCDWR) ; UT Long Creek , SR 1446, 35.31750, - 81.27194, 5 April 1994, N. Guthrie, 1L ( NCDWR) . Graham Co., Little Snowbird Creek , off SR 1115, 35.28697, -83.87841, 4 June 2014, M. Walters, 1L ( NCDWR) . Henderson Co., Mills River, SR 1353, 35.38778, -82.54611, 2 May 2011, T. Mormon, 2L ( NCDWR) ; same location, 23 April 2012, E. Fleek, 1L ( NCDWR) ; Mills River , NC 191 /280, 35.39139, -82.56806, 2 June 2009, T. Mormon, E. Fleek, M. Walters, 4L ( NCDWR) ; North Fork Mills River, SR 1343, 35.39425, -82.61503, 3 May 2011, T. Mormon, 1L ( NCDWR) ; South Fork Mills River , SR 1340, 35.37569, -82.6144, 1 June 2009, T. Mormon, E. Fleek, M. Walters, 1L ( NCDWR) . Iredell Co., Olin Creek , SR 1891, 35.94472, -80.84444, 9 May 2006, D. Black, B. Prusha, S. Beaty, 3L ( NCDWR) ; Patterson Creek , SR 1890, 35.92417, -80.82417, 4 April 2006, E. Fleek, 2L ( NCDWR) ; Rocky Creek , SR 1854, 35.99139, -80.85944, 5 June 2003, D. Lenat, 4L ( NCDWR) ; Third Creek , SR 2318, 35.73889, -80.84444, 21 June 1989, D. Lenat, 1L ( NCDWR) ; Third Creek , SR 1521, 35.80917, -81.00222, 8 May 2006, S. Beaty, D. Black, 1L ( NCDWR) . Jackson Co., [Savannah Creek] Fort Tatham Campground , [ US 441], [35.29354, -83.26746], 8 July 1981, B.C. Kondratieff, 1F ( INHS, Insect Collection 875913) . Lincoln Co., Howards Creek, SR 1200, 35.49833, -81.34056, 3 May 2006, B. Crouch, 2L ( NCDWR) ; Killian Creek , SR 1511, 35.41583, -81.02889, 8 June 1994, D. Lenat, 2L ( NCDWR) ; Leepers Creek , SR 1354, 35.49861, -81.14619, 8 May 2006, B. Prusha, 4L ( NCDWR) ; Pott Creek, SR 1217, 35.55194, -81.31889, 3 May 2006, T. Mormon, 1L ( NCDWR) . Macon Co., Burningtown Creek, Tellico Rd , 35.28277, -83.48240, 6 May 2008, T. Mormon, 1L ( NCDWR) ; Caler Fork, off Ruby Mine Rd , 35.26860, -83.38656, 7 May 2008, R. Thorpe, 1L ( NCDWR) ; Coon Creek , US 23/441, 35.23033, -83.34921, 9 May 2008, T. Mormon, 1L ( NCDWR) ; Cowee Creek, Leatherman Gap Rd , 35.27046, -83.39047, 8 May 2008, R. Thorpe, 3L ( NCDWR) ; Cowee Creek , NC 28 , 35.2625, -83.40944, 23 May 2007, D. Black, 1L ( NCDWR) ; Downes Branch , SR 1392, 35.21263, -83.49833, 28 April 2011, V. Holland, 1L ( NCDWR) ; Iotla Branch, SR 1387, 35.22425, -83.42923, 7 May 2008, R. Thorpe, 3L ( NCDWR) ; Iotla Creek , SR 1385, 35.21887, -83.42293, 7 May 2008, C. Tyndall, 2L ( NCDWR) ; Iotla Creek , off SR 1378, 35.23473, -83.39824, 24 May 2007, D. Black, 2L ( NCDWR) ; same location, 7 May 2008, T. Mormon, 1L ( NCDWR) ; Mica City Creek , SR 1346, 35.2696, -83.37045, 25 April 2011, V. Holland, 3L ( NCDWR) ; North Fork Coweeta Creek , SR 1114, 35.06525, -83.40253, 17 May 2011, V. Holland, 2L ( NCDWR) ; Rabbit Creek , off SR 1504, 35.20690, -83.34836, 8 May 2008, R. Thorpe, 12L ( NCDWR) ; Rabbit Creek, SR 1504, 35.20837, -83.35209, 8 May 2008, T. Mormon, 3L ( NCDWR) ; Younce Creek, Younce Creek Rd. , 35.23775, -83.47404, 26 April 2011, V. Holland, E. Fleek, S. Beaty, 1L ( NCDWR) . McDowell Co., Catawba River, SR 1273, 35.61944, -82.18833, 18 April 1985, NCDWR, 1L ( NCDWR) ; Catawba River , SR 1234, 35.63694, -82.14389, 18 April 1985, NCDWR, 1L ( NCDWR) ; North Muddy Creek , SR 1760, 35.67528, -81.90639, 17 April 1985, NCDWR, 16L ( NCDWR) ; Youngs Fork , SR 1819, 35.65389, -81.96306, 17 April 1985, NCDWR, 1L ( NCDWR) . Nash Co., Sandy Creek, SR 1405, 36.12694, -78.02472, 21 April 2003, E. Fleek, 3L ( NCDWR) ; Swift Creek , SR 1310, 36.11167, -77.92111, 24 April 2003, E. Fleek, 2L ( NCDWR) . Polk Co., Rotten Creek, off Jeep Trail 35.34709, -82.21117, 16 April 2014, V. Holland, 1L ( NCDWR) ; Whiteoak Creek , SR 1 352, 35.26055, -82.00388, 15 May 1995, N. Guthrie, L. Eaton, K. Herring, 1L ( NCDWR) . Richmond Co., Naked Creek, SR 1003, 35.08194, -79.59028, 6 April 1990, L. Eaton, 2L ( NCDWR) ; Naked Creek , SR 1003, 35.08197, -79.58909, 18 April 2013, D. Lenat, 1L ( NCDWR) ; Rocky Ford Branch, SR 1424, 35.12417, -79.66250, 9 May 1990, D. Lenat, 8L ( NCDWR) . Rockingham Co., Falls Creek, Mayo River State Park , 36.539178, -79.988084, 1 May 2012, D. Lenat, 1L ( NCDWR) ; Rock House Creek , SR 2127, 36.42083, -79.79055, 12 April 2001, L. Eaton, 1L ( NCDWR) . Rutherford Co., Second Broad River, above Union Mills , 35.47917, -81.94417, 19 May 1999, K. Herring, 4L ( NCDWR) ; Second Broad River, below Union Mills , 35.47667, -81.94250, 19 May 1999, K. Herring, 1L ( NCDWR) Second Broad River , SR 1504, 35.48401, - 81.94921, 18 May 2010, D. Black, S. Beaty, E. Fleek, 1L ( NCDWR) . Stokes Co., Brushy Fork, SR 1998, 36.32306, -80.30278, 18 May 2004, N. Flint, 1L ( NCDWR) ; East Prong Little Yadkin River , SR 1224, 36.33126, -80.38586, 17 May 1994, N. Guthrie, 4L ( NCDWR) ; Little Yadkin River , SR 1104, 36.29889, -80.41389, 18 May 1994, D. Penrose, 1L ( NCDWR) ; same location 31 May 1989, D. Penrose, 1L ( NCDWR) ; Little Yadkin River , SR 1104, 36.29899, -80.41388, 6 May 1987, D. Penrose, 2L ( NCDWR) ; same location, 31 May 1989, D. Penrose, 1L ( NCDWR) . Surry Co., Endicott Creek, Raven Knob Rd. , 36.47274, -80.85152, 5 May 2010, V. Holland, M. Walters, 2L ( NCDWR) ; Fisher River , nr SR 1346, 36.41806, -80.71752, 4 May 2010, V. Holland, M. Walters, 4L ( NCDWR) . Transylvania Co., Catheys Creek, SR 1338, 35.20750, -82.78056, 21 May 1987, D. Penrose, 1L ( NCDWR) . Wilkes Co., Elk Creek, SR 1175, 36.10639, -81.43808, 7 June 2006, M. Walters, 1L ( NCDWR) ; Left Prong Stony Fork , off SR 1155, 36.15065, -81.45392, 20 April 2012, S. Beaty, 1L ( NCDWR) ; North Prong Lewis Fork , nr SR 1300, 36.21309, -81. 35806, 7 May 2010, V. Holland, M. Walters, 2L ( NCDWR) ; Yadkin River, nr Abtco Rd. , 36.19694, -81.02917, 7 June 1993, D. Penrose, 1L ( NCDWR) . Yadkin Co., North Deep Creek , NC 601 , 36.16861, -80.68111, 12 April 1993, D. Lenat, 6L ( NCDWR) . South Carolina: Aiken Co., Tinker Creek, Savannah River Site , SRP B-1, [33.33769, -81.60466], 1 June 1977, Herlong, Prichard, 3F ( NCDWR) ; same data, 40M, 7F ( INHS, Insect Collection 875859) ; same data, 4M, 119F ( INHS, Insect Collection 875917) ; same data, 136F ( INHS, Insect Collection 875919) ; same data 1M, 62F ( INHS, Insect Collection 875902) ; same data, 1M, 165F ( INHS, Insect Collection 875845) ; same data, 1M, 100F ( INHS, Insect Collection 875868) ; same location, 28 June 1977, Herlong, Prichard, 5F ( NCDWR) ; Upper Three Runs , Savannah River Site Rd. C, [33.28518, -81.69533], 5 May 1984, B.C. Kondratieff, 29M, 8F ( NCDWR) ; same location, 27 May 1984, B. Kondratieff, 1M, 27F ( NCDWR) ; same location, 4 June 1984, B. Kondratieff, 14M, 26F ( NCDWR) ; same location, 17 April 1985, B. Kondratieff, 2M, 1E ( NCDWR) ; Upper Three Runs , Savannah River Site Rd. F, [33.23926, -81.74427], 4 May 1977, Herlong, Prichard, 4M, 1F ( INHS, Insect Collection 875918) ; same location, 17 May 1977, Herlong, Prichard, 12M, 5F ( INHS, Insect Collection 875916) ; same location, 1 June 1977, Herlong, Prichard, 3M, 159F ( INHS, Insect Collection 875847) ; same data, 215F ( INHS, Insect Collection 875861) . Anderson Co., Rock Creek , N of S-4-670, [34.56723, -82.53546], 3–4 June 1980, J. Weaver, 1M ( NCDWR) . Barnwell Co., Meyers Branch , 600 m upstream from Savannah River Site Rd. A, [33.15049, -81.62530], 3 June 1983, B. Kondratieff, 5F ( NCDWR) ; same location, 3 June 1983, B. Kondratieff, 4F ( NCDWR) ; Steel Creek , Savannah River Site Rd. A, [33.14589, -81.62874], 3 June 1982, B. Kondratieff, 2M, 1F, 2E, ( NCDWR) ; same data, 1M, 4F ( NCDWR) . Oconee Co., Chauga River, 4 mi S. of Mountain Rest , [34.83317, -83.15801], 10 June 1969, J. Morse, 1M, 2F ( INHS, Insect Collection 875857) . Pickens Co., Wildcat Creek , 9 km NW Clemson, [34.75589, -82.85776], 25 May 1980, J.S. Weaver III, 1F ( INHS, Insect Collection 875870) ; South Saluda River , 4 mi N Pumpkintown, [35.06719, -82.61793], 27 June 1969, H. Douglas, Fox & Fox, 2M, 1F ( INHS, Insect Collection 875858) . Spartanburg Co., South Saluda River , 5 mi W Cleveland, [35.06759, - 82.61827], 2 June 1969, J. Morse, G. Townes, 2M ( INHS, Insect Collection 875850) ; same location, 2 July 1969, H. Douglass, G. Townes, 1F ( INHS, Inset Collection 875899) . Tennessee: Blount Co., Abrams Creek, 100 m upstream Abrams Falls Trailhead, Great Smoky Mountains National Park , 35.59210, -83.85200, 8 June 2001, B.D. Heinold, 5F ( INHS, Insect Collection 3383) ; same location, 6 July 2001, B.D. Heinold, R.E. DeWalt, S.L. Johnson, 1F ( INHS, Insect Collection 3457) . Virginia: Franklin Co., Big Chestnut Creek, Rte 718, [36.87630. W 79.86254], 21 May 2002, B.C. Kondratieff, R. F. Kirchner, 2M ( VMNH) ; Grassy Fork , 2 mi S of Snow Creek, [36.81097, - 79.74516], 2 June 2000, R. L. Hoffman, 3M, 8F ( VMNH) ; Pigg River, 0.3 km upstream of Rte 890 bridge SW Penhook , [36.94638, -79.64051, 26 June 2005, R. L. Hoffman, 1M, 7F ( VMNH) ; Shooting Creek, Rte 860, 1.4 mi W jct. VA 40, [36.88060, -80.20147], 13 July 1991, R. L. Hoffman, 1F ( VMNH) . Halifax Co., no waterbody data, Berry Hill ( Delorme 27? D5-6) at light in logged floodplain, [no coordinates], 8 June 1993, R. L. Hoffman, C. Hampton, 1F ( VMNH) ; no waterbody data, swamp 2.5 mi E of Riceville , [no coordinates], 21 May 2000, ( VMNH) survey, 1M ( VMNH). Pittsylvania Co., Banister River, below Mt.Airy Mill, Rte 683, ca 1 mi SW Mt. Airy, [36.93180, -79.20300], 25 June 1989, R. L. Hoffman, 1M ( VMNH) ; Crooked Run, Rte 841, 6 mi SW Callands, [36.74825, - 79.65121] 9 June 2000, R. L. Hoffman 3M, 5F ( VMNH) ; Sandy River, Rte 845 bridge 4.5 mi N Brosville, [36.67935, -79.59389], 24 June 1993, R. L. Hoffman, 1F ( VMNH) ; South Prong Sandy River , NW of Brosville, [36.66365, - 79.61798], 19 June 1990, R. L. Hoffman, 1F ( VMNH) ; [ Bawley Branch ], jct. 939 & 614 3 mi SE of Sandy River, [36.71343, -79.61181], 2 June 1998, R. L. Hoffman, 1M, 2F ( VMNH) .

Figures

FIGURE 15. Distribution of Isoperla riverae sp. n. and examined material of I. dicala and I. lenati. Records of I. lenati from Szczytko & Kondratieff are also included.

FIGURES 16–18. Isoperla riverae sp. n., adult male. 16. Head and pronotum, dorsal, Drowning Creek, Moore/Richmond Co., North Carolina. 17–18. Grassy Fork, Franklin Co. Virginia. 17. Vesicle, ventral. 18. Paraprocts, caudal.

FIGURES 19–21. Isoperla riverae sp. n., adult male terminalia, (A) posteroventral lobe, (B) posterior lobe, (C) pair of small caudal lobes, (D) conical apical lobe, (E) paired hemispherical anterior lobes, Mayo River, Rockingham Co., North Carolina. 19. Lateral, 20. Dorsal, 21. Ventral.

FIGURES 22–25. Isoperla riverae sp. n., adult male, aedeagus, (A) posteroventral lobe, (B) posterior lobe, (C) pair of small caudal lobes, (D) conical apical lobe, (E) paired hemispherical anterior lobes, Drowning Creek, Moore/Richmond Co., North Carolina. 22. Lateral. 23. Details of fig. 22 inset, hair-like spinulae, sensilla basiconica and small rounded knobs with apical filaments. 24. Sharp spinulae and hair-like spinulae of posteroventral lobe (A) 25. Small mounds with sharp spinulae on posterior lobe (B).

FIGURES 26–27. Isoperla riverae sp. n., adult female, Drowning Creek, Moore/Richmond Co., North Carolina. 26. Habitus, dorsal. 27. Subgenital plate, ventral.

FIGURES 28–31. Isoperla riverae sp. n., ovum, Drowning Creek, Moore/Richmond Co., North Carolina. 28. Convex aspect. 29. Concave aspect. 30. Reticulation of egg chorion and globules. 31. Eclosion line and micropyles.

FIGURES 32–35. Isoperla riverae sp. n., larva, Naked Creek, Richmond Co., North Carolina. 32. Habitus, dorsal. 33. Head and pronotum, dorsal. 34. Abdomen, dorsal. 35. Abdomen, ventral.

FIGURES 36–39. Isoperla riverae sp. n., larva. 36–37. Naked Creek, Richmond Co., North Carolina. 36. Right lacinia, ventral. 37. Left lacinia, ventral. 38–39. Rocky Ford Branch, Richmond Co., North Carolina. 38. Right mandible, ventral; (inset) acanthae. 39. Left mandible, ventral; (inset) dense brush of short spinous setae.