Pannonibacter carbonis, Xi & Qiao & Liu & Li & Zhang & Liu, 2018

Xi, Lijun, Qiao, Nenghu, Liu, Dejian, Li, Jing, Zhang, Jingjing & Liu, Jianguo, 2018, Pannonibacter carbonis sp. nov., isolated from coal mine water, International Journal of Systematic and Evolutionary Microbiology 68 (6), pp. 2042-2047 : 2046

DESCRIPTION OF PANNONIBACTER CARBONIS SP. NOV.

Pannonibacter carbonis (car.bo′ nis. L. gen. n. carbonis of coal, of charcoal).

Cells are facultatively anaerobic, Gram-stain-negative, rod shaped, motile by means of a single polar flagellum and contain PHA. They are 0.3 – 0.7 µm wide and 1.8 – 3.0 µm long. Colonies grown on TSA plates are smooth, cream-coloured and approximately 1 – 1.6 mm in diameter. They do not produce BChl a. Cells grow in TSB in the presence of 0 – 5.5 % (w/v) NaCl (optimum 0 – 2.0 %), at 4 – 55 Ǫ C (optimum 30 – 35 Ǫ C) and at pH 3.5 – 10.5 (optimum 6.5 – 8.0). They are positive for catalase, oxidase, urease, VP test, gelatin hydrolysis and H 2 S production, but negative for nitrate reduction, indole production and Tween 80 hydrolyzation. D- Galactose, D- glucose, maltose, trehalose, o-nitrophenyl-b- D- galactoside, arginine, lysine, ornithine, sodium citrate, sorbitol, mannitol, potassium gluconate, wood sugar and xylitol can be used as a sole carbon source; however, arabinose, erythritol, mannose, lactose, adipic acid, inositol and starch cannot be used as a sole carbon source. Sucrose is weakly assimilated. Acid is aerobically produced from adipic acid, D- galactose, D- glucose, sorbitol, xylitol, mannose, lactose, potassium gluconate and wood sugar. The major cellular fatty acid is C 18: 1 Ɯ7c. The principal quinone is Q-10. Polar lipids include diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline, an unidentified amino lipid, two unidentified phospholipids and one unidentified lipid.

The type strain is Pannonibacter carbonis Q4.6 T (=CGMCC 1.15703 T =KCTC 52466 T), which was isolated from a coal mine water sample collected from a coal-producing well in Jinchen , Shanxi Province, China. The DNA G+C content is 63.6 mol%.