Roseovarius ramblicola, Castro & Cerezo & Sampedro & Martínez-Checa, 2018

Castro, David J., Cerezo, Isabel, Sampedro, Inmaculada & Martínez-Checa, Fernando, 2018, Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain, International Journal of Systematic and Evolutionary Microbiology 68 (6), pp. 1851-1856 : 1855

DESCRIPTION OF ROSEOVARIUS RAMBLICOLA SP. NOV.

Roseovarius ramblicola [ram.bli′ co.la. Spanish fem. n. rambla sandy ground; L. suff. -cola (from L. masc. or fem. n. incola) inhabitant; N.L. n. ramblicola inhabitant of a rambla].

Cells are ovoid- or rod-shaped, Gram-stain-negative, nonmotile, 0.7–1.2×2.1–3.2 µm in size and reproduce by budding or asymmetrical division. Cell colonies are white, circular, convex and opaque when grown on MA and R2A media. The growth pattern is uniform in a liquid medium. Capable of growing in NaCl concentrations of 0.5 to 10 % (w/v), with optimum growth occurring at 3 %. Grows within a temperature range of 5–30 Ǫ C at pH values of between 6 and 9, the optimum values being 28 Ǫ C and pH 7. Catalase and oxidase are produced. Nitrate reduction, nitrite reduction, indol production, hydrolysis of arginine, aesculin and gelatine are negative. Urease is not produced. In API 20NE assays, assimilation of D- glucose, maltose, L- arabinose, mannose, N -acetyl-b- glucosamine, gluconate, adipic acid, malic acid, phenylacetic acid, capric acid and trisodium citrate are negative. In API ZYM assays, leucine arylamidase is positive, but esterase C4, esterase lipase C8, naphthol-AS-BIphosphohydrolase and alkaline phosphatase lipase C14, valine arylamidase, cystine arylamidase, trypsine, a- chymotrypsin, a- galactosidase, b- galactosidase, b- glucuronidase, a- glucosidase, b- glucosidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase are negative. Acid is not produced from sugar in API 50 CH. It is susceptible to amoxicillin, amoxicillin/clavulanic acid, ampicillin, azithromycin, aztreonam, bacitracin, carbenicillin, cefotaxime, cefalotin, cefoxitin, cefuroxime, chloramphenicol, erythromycin, rifampicin, penicillin, tobramycin, trimethoprim/ sulfamethoxazole, norfloxacin, sulfonamides and streptomycin. Resistant to gentamicine, nalidixic acid, polymyxin B, ciprofloxacin, tetracyclin, amikacin and neomycin.

The only detected lipoquinone is ubiquinone with ten isoprene units (Q10). The DNA G+C content of the type strain is 63 mol% (Tm method). The major fatty acids (>5 % of the total fatty acids) of strain D15 T are C 18: 1 Ɯ 7 c, C16: 0 and C12: 0.

The polar lipids contain phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three unidentified polar lipids. The type strain, D15 T (= CECT 9424 = LMG 30322), was isolated from a saline soil sample from Rambla Salada (Murcia) south-eastern Spain .