Mideopsis roztoczensis Biesiadka & Kowalik, 1979

Pešić, Vladimir, Bańkowska, Aleksandra, Zawal, Andrzej, Rewicz, Tomasz & Smit, Harry, 2023, Molecular diversity and species delimitation in the genus Mideopsis Neuman, 1880 in Europe (Acari, Hydrachnidia, Mideopsidae), Ecologica Montenegrina 70, pp. 101-115 : 108

Mideopsis roztoczensis Biesiadka & Kowalik, 1979

Remarks — Mideopsis roztoczensis was originally described by Biesiadka & Kowalik (1979) from Szum River, Biała Łada River, Wieprz River and their tributaries in Roztocze area, east-central Poland. After that, it has been reported from different parts of Europe ( Gerecke et al. 2016) and Türkiye ( Pešić et al. 2022a). According to the original description by Biesiadka & Kowalik (1979), Mideopsis roztoczensis in the first line is characterized by a more elevated dorsal shield with distinctly visible anteriorly diverging lines of particularly faint fine porosity ( Fig. 3A ), and the shape of the male ejaculatory complex with the wedge-shaped anterior ramus being wider, with a characteristic arrow-shaped delimited area - see Figure 3K ).

In the analysed material, M. roztoczensis was represented by 21 barcoded individuals assigned to four BINs ( Fig. 2 ). BIN ACI1492, which is the most widespread in Europe, contained most of the sequences from Montenegro (n = 5), Norway (n = 5), Russia (n =1), Bosnia and Herzegovina (n = 3), Türkiye (n = 4), Serbia (n = 1), and Cyprus (n = 1). BIN AEN6785 consists of sequences from Türkiye, Serbia, and Northern Europe ( Norway). The BIN AEA2936 includes sequences from Serbia and two from Belgium, while BIN AEO2944 contains sequences from Corsica deposited in BOLD. Some BINs, were found together at the same locality (e.g., BIN ACI1492 and BIN AEN 6785 in Katušnica River in Serbia).

Figures

Figure 2. Minimum Spanning Network showing phylogenetic relationships within three Mideopsis clades: M. roztoczensis, M. orbicularis Clade 1, and M. orbicularis Clade 2. Each bar represents a single mutational change, and a dashed line encircled by separate BINs. The diameter of the circles is proportional to the number of individuals in each haplotype sampled (see open circles with numbers).

Figure 3. A-D Photographs of dorsal shield: A – M. roztoczensis, ♂, CCDB38233 C11, River Zeta, Montenegro. B – M. orbicularis Clade 1, ♂, River Bukowa, Poland. C – M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. D – M. orbicularis, ♂, Tjeukemeer, Netherlands. E-H Genital field: E – M. roztoczensis, ♂, CCDB38233 C11, Montenegro. F-G – M. orbicularis Clade 1, ♂: F – River Bukowa, Poland; G – RMNH.ACA.374, Netherlands. H-I – M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. K-O Ejaculatory complex. K – M. roztoczensis, ♂, Roztocze, Poland (from Biesiadka & Kowalik 1979: fig. 10), inset: photograph of anterior ramus, CCDB38233 C11, Montenegro). L – M. orbicularis Clade 1, Roztocze, Poland (from Biesiadka & Kowalik 1979: fig. 12). M – M. orbicularis Clade 1, ♂, River Bukowa, Poland (inset: photograph of anterior ramus). N – M. milankovici, paratype ♂, Montenegro (from Pešić & Smit 2020). O – M. orbicularis, ♂, Tjeukemeer, Netherlands. Scale bars = 100 µm (for K-L = 50 µm).