Amanita minqingensis Jun Z. Qiu, 2024

Guan, Xiayu, Zhu, Mengjia, Dang, Yuxiao, Lv, Huajun, Wu, Ziyi, Heng, Zhiang, Chen, Ruiya, Liu, Sen, Lai, Pengyu, Yang, Chenjie, Chen, Jinhui & Qiu, Junzhi, 2024, Amanita minqingensis, a novel taxon of Amanita section Vaginatae (Amanitaceae, Agaricales) from Southern China, Phytotaxa 664 (2), pp. 85-97 : 93-94

Amanita minqingensis Jun Z. Qiu , sp. nov. Figures 1 , 2 and 3

MycoBank: MB851856.

Etymology:—Named after Minqing District, where the fungus was collected.

Diagnosis: Differs from related species mainly in having tan pileus with putty verrucose volval remnants, with globose to subglobose basidiospores.

Holotype:— CHINA. Fujian Province, Minqing District, Huangchulin Nature Reserve , in coniferous and broadleaved mixed forest, 26°15′29″N, 118°39′38″E, altitude 969 m, 14 May 2021 (holotype HMAS298096 ).

Description: Basidiomata medium-sized. Pileus 30 - 60 mm in diam., initially hemispherical, expanded to be convex in maturity dull, dry, slightly viscid when moist, tan (6E6), same tan (6E6) color toward margin; volval remnants on pileus warty to verrucose, 0.5‒1.5 mm high, 2‒5 mm wide, putty (4B2); margin striate with dark brown (5E8) color, non-appendiculate; context 3‒5 mm thick above stipe, soft, white to whitish (1A1), unchanging. Lamellae free, crowded, white to whitish (1A1); lamellulae abundant, truncate. Stipe 60 × 7 mm diam., slightly tapering upwards, with apex slightly expanded, smooth, white to whitish (1A1), unchanging when bruised; context white to whitish (1A1), hollow in center, soft, unchanging when cut; basal bulb absent. Universal veil on stipe base show volval remnants, arranged in incomplete rings, the residue white to whitish (1A1). Partial veil absent. Spore print not recorded. Odor indistinct.

Lamellar trama bilateral. Mediostratum 20–50 µm wide, composed of abundant, clavate inflated cells (10–20 × 8–20 µm); filamentous hyphae abundant, 2–6 µm wide; vascular hyphae scarce. Basidia 24‒43 × 10‒15 μm, clavate, 4- spored; sterigmata 3‒5 μm long, clamp connection absent. Basidiospores (n = 40) (9‒)9.2‒10.3‒11 × (8.5‒)9‒9.5‒10.5 μm, Q=1.05‒1.15 (1.18), Qm=1.10±0.05, globose to subglobose, inamyloid, colorless, thin‒walled, smooth; apiculus small, 0.5-1.5 μm. Lamellar edge appearing as a sterile strip, composed of subglobose, ellipsoid to clavate inflated cells, 20–30 × 10–20 µm, single and terminal or in chains of 2–3 inflated cells, thin-walled, colorless; filamentous hyphae abundant, 2–6 µm wide, irregularly arranged or ± running parallel to lamellar edge. Pileipellis composed of interwoven to radially arranged, thin-walled, colorless, filamentous hyphae 4.5‒8 μm wide; Volval remnants on pileus composed of irregularly arranged, fairly abundant filamentous hyphae, 2‒6 μm; inflated cells abundant to very abundant, oval, 27‒35 × 20‒27 μm, colorless, thin‒walled. Clamp connection absent in all parts of basidioma.

Additional specimen examined: CHINA. Fujian Province, Minqing District, Huangchulin Nature Reserve, in coniferous and broad-leaved mixed forest, 26°15′29″N, 118°39′38″E, altitude 969 m, 14 May 2021 (paratype HMAS298097).

Habitat: Solitary in coniferous and broad-leaved mixed forest. The species is found in summer from May to September.

Distribution: Presently known from Fujian province of China.A summary of the locations of isolation, ecological habitats, and main morphological characteristics of Amanita found in the Fujian province is given (Table 2).

Figures

FIGURE 1. Phylogeny inferred from multigene sequences (nrLSU, ITS, rpb2 and tef1–α) using the Bayesian analysis. Bootstrap values (BI/ML) over 0.5/90 are shown above the individual branches. The scale bar indicates the number of nucleotide substitutions per site. New species is highlighted in red and bold. Arrows show the support values at the branching points. Superscript “T” denotes the type strain of the new species.

FIGURE 2 Morphological characteristics of Amanita minqingensis (HMAS298096, holotype). (a) Basidiocarps in natural habitat, (b) lamellae characteristics. Scale bars: a, b = 10 mm.

FIGURE 3 Microscopic features of Amanita minqingensis (HMAS298096). A. Hymenium and subhymenium; B. Volval remnants on the pileus; C. Pileipellis; D. Basidiospores. Scale bars: A‒D = 10 µm.