Chaetosphaeronema barriae (Shoemaker) Mlčoch 2024

Mlčoch, Patrik & Matušinsky, Pavel, 2024, Phylogenetic and morphological revision sexual stages of the genus Paraphoma (Phaeosphaeriaceae) and next related species from clade of Ophiobolus-like (Phaeosphaeriaceae), Phytotaxa 663 (4), pp. 184-204 : 196

Chaetosphaeronema barriae (Shoemaker) Mlčoch comb. nov. — Fig. 6 .

= Leptosphaeria barriae Shoemaker 1985

TYPE: CANADA: ALBERTA: 1801 26, Lupinus sp. , near Crandell campsite, Waterton National Park, 49’06’ N, 1 13’5.5’ W, K. N. Egger 463, 31 July 1980, 180128, idem K. N. Egger 559, 6 Aug. 1980.( Shoemaker 1984).

Epitype: BRNM 840266

MycoBank no: 854609

Sexual morph. Ascoma pseudothecial, semiglobose to conical, in numerous groups, subepidermal, 450–500 μm in diam. and to 250 μm height, later semi-immersed to erumpent by ostioles. Ostioles are inconspicuous, small or truncate and conical, to 75 μm height and to 150 μm in diam. Ascomal wall composed of 6 to7 layers of pseudoparenchymatous cells, composed of brown, polygonate or isodiametric cells of textura angularis, (6)8–9(11.5) × (4.5)5–7.5(8.5) μm, N =20. Asci bitunicate, 8–spored, cylindrical, short pedicellate, 80–105 × 8–11.5 μm, N =10. Ascospores narrowly fusiform, bi- or triseriate in ascus, rare to tetraseriate, firstly hyaline, later yellow to yellowish brown, 6 to 7–septate, third or rare fourth cell of spore from apex enlarged towards base, central cells constricted to first septum, smooth, without appendages gelatinous sheath or ornamentation, with or without lipid drops, (44.5)45.5–55.5(60) × (4.5)5– 7(8) μm, Q =7.5–9.8, Q AV =8.4, N =46. Asexual morph. Conidiomata globose, pycnidial, to 500 μm in diam. and to 340 μm on the height. Ostiole short, to 60 μm in diam. and 25 μm on the hight. Conidiomatal wall composed of several layers of brown cells of textura angularis, 7.7–9 × 5–6.5 μm, N =20. Conidiogenous cells in the preparate were not found. Conidia ellipsoid with rounded ends, hyaline, aseptate, smooth-walled, 6–8(–8.2) × (1.1–) 1.3–1.8 (–2) μm, Q =3.8–5.4, Q AV =4.7, N =20

Culture characters. Colony grown on the PDA very fast, to 40 mm in diam. after two weeks at 25 ° C. Colony circular, primarily whitish to greyish, later faintly brownish to yellowish grey, with raised to flat elevation and entire margin. Conidiomata and conidia undetermined.

Habitat. Dead stems of Lupinus ssp.

Distribution. Europe (this study) and North America ( Shoemaker 1984). Taxon is bound to distribution its Lupinus polyphyllus , so probably it was introduced into Europe together with the host plant.

Material examined: SLOVAKIA: 1. col. P. Mlčoch, High Tatras, Starý Smokovec-Hrebianok, over the Vodopády Studeného potoka falls, the edge of the tourist path with invasive vegetation of Lupinus polyphyllus , 1350 m a. s. l., 5. 7. 2020, on the dead stems of Lupinus polyphyllus, GPS : 49.1620506N, 20.2213011E ( BRNM 840266, GenBank no. OR 878083). AUSTRIA: 1. col. P. Mlčoch, Austrian Alps, St. Michael im Lungau, Katschberg, 1650 m a. s. l., 26. 7. 2019, on the dead stems of Lupinus polyphyllus ( BRNM 840264, BRNM 840265); THE CZECH REPUBLIC: 2. col. P. Mlčoch, Hrubý Jeseník Mts., Červenohorské sedlo, chalet, 1040 m a. s. l., 10. 8. 2017, on the dead stems of Lupinus polyphyllus ( BRNM 840263).

Notes. This species, descripted by Shoemaker, is very good defined and distinguishable from related species (see in key next). Cultivate from the collection BRNM 840266 report of 99 % identity by Chaetosphaeronema achilleae , Ch. clematidis , Ch. clematidicola , Ch. hispidulum and Ophiobolus cirsii in ITS and LSU genes. Morphologically, there species are different.

Figures

FIGURE 6. Chaetosphaeronema barriae (BRNM 840266). Q–R: ascoma; A: ascomal wall; G, H: asci; B–F, I–M: ascospores; L— pycnidial wall; P—conidia. Scales: B–D, G–H, J–O: 10 μm; A, E–F, I, P: 5 μm; Q–R: 250 μm.