Wickerhamomyces menglaensis, Chai & Huang & Cheng & Liu & Hui, 2019

Chai, Chun-Yue, Huang, Lin-Na, Cheng, Han, Liu, Wen-Jing & Hui, Feng-Li, 2019, Wickerhamomyces menglaensis f. a., sp. nov., a yeast species isolated from rotten wood, International Journal of Systematic and Evolutionary Microbiology 69 (5), pp. 1509-1514 : 1512-1513

DESCRIPTION OF WICKERHAMOMYCES MENGLAENSIS HUI & HUANG SP. NOV.

Wickerhamomyces menglaensis ( meng.la.en′ sis. N.L. masc. adj. menglaensis of or belonging to the city of Mengla , Yunnan Province, PR China, the collection locality of the type strain of the species) .

In YM broth after 3 days at 25 Ǫ C, the cells are ellipsoidal to elongate (3.0–6.7×5.9–8.2 µm) and occur singly or in pairs. Budding is multilateral ( Fig. 2a ). On YM agar after 3 days at 25 Ǫ C, the streak culture is butyrous, cream and convex with a smooth surface and has an entire margin. On Dalmau plates after 13 days on YCBS agar at 25 Ǫ C, pseudohyphae are formed ( Fig. 2b ), but true hyphae are not formed. Ascospores are not observed on YM, 5 % malt extract, cornmeal and YCBAS agar in pure and mixed cultures at 17 and 25 Ǫ C for up to 4 weeks. Glucose and raffinose (weak) are fermented. No fermentation of the following carbohydrates: galactose, sucrose, maltose, lactose, trehalose, D- xylose, cellobiose and melezitose. Assimilation of carbon compounds is as follows: D- glucose, D- xylose (weak), L- arabinose (weak), L- rhamnose (weak), cellobiose, salicin, arbutin, glycerol (weak), starch (weak), inulin (weak), D- glucitol, D- mannitol, D- glucono-1, 5-lactone, DL- lactate, succinate and citrate. Carbon sources not assimilated are as follows: D- galactose, L- sorbose, D- glucosamine, D- ribose, D- arabinose, sucrose, maltose, trehalose, melibiose, lactose, raffinose, melezitose, erythritol, ribitol, xylitol, L- arabinitol, galactitol, myo -inositol, 2-keto-D- gluconate, methanol, D- gluconate, D- glucuronate, D- galacturonate, 5-keto-D- gluconate and ethanol. Assimilation of nitrogen compounds are as follows: nitrate, nitrite, L- lysine (weak), glucosamine (weak) and D- tryptophan (weak). Cadaverine, creatine, creatinine, ethylamine and imidazole are not assimilated. Cell growth is observed in vitamin-free medium. Growth is observed at 35 Ǫ C. No growth on 50 % (w/v) glucose or 5 % (w/v) glucose/sodium chloride (10 %). Urea hydrolysis and diazonium blue B reactions are negative.

The holotype, NYNU 1673 , was isolated in August 2016, from rotting wood collected in Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. It is preserved in a metabolically inactive state at Microbiology Lab, Nanyang Normal University, Henan, PR China. Ex-type cultures are deposited at the China Center of Industrial Culture Collection (CICC), Beijing, PR China, as strain CICC 31159 , and at the Yeast Collection of the Westerdijk Fungal Biodiversity Institute (CBS), Utrecht, The Netherlands, as strain CBS 14689 . The MycoBank number is MB 829372.

Figures

Fig. 2. Photomicrographs of Wickerhamomyces menglaensis f.a., sp. nov. NYNU 1673. (a) Budding cells grown on YM broth for 3 days at 25 ǪC. (b) Pseudohyphae grown on YCBS agar for 13 days at 25 ǪC. Bar, 10 µm.