Typhlodromus (Typhlodromus) setubali Dosse, 1961

ZahidiK, Abdelaziz, Akchour, Abdellah, K, Serge Kreiter, TixierK, Marie-Stéphane, MsandaK, Fouad & MousadikK, Abdelhamid El, 2023, Phytoseiid mites (Acari: Mesostigmata) from Central West Morocco: new records and key to females of all recorded Moroccan species, Acarologia 63 (3), pp. 691-724 : 709-713

Typhlodromus (Typhlodromus) setubali Dosse

Typhlodromus setubali Dosse 1961: 313 . Typhlodromus (Typhlodromus) setubali, Moraes et al. 1986: 246 , 2004a: 369 ; Chant & McMurtry 2007: 157. Typhlodromus laurentii, Ragusa & Swirski 1978: 213 (Synonymy according to Chant & Yoshida-Shaul 1987).

World distribution — Germany, Israel, Italy, Jordan, Madeira Island ( Portugal), Morocco, Portugal, Slovakia, Spain, and Tunisia.

Previous records from Morocco — specimens were collected on several plants such as Quercus suber L. ( Fagaceae ), Nerium oleander L. ( Apocynaceae ), Rosmarinus officinalis L. ( Lamiaceae ), Marrubium incanum L. ( Lamiaceae ), Cistus spp. L. ( Cistaceae ), and Thuya spp. L. ( Cupressaceae ) in Damon Oum Er Bia, Beni Slimane, and Ras El Ma regions ( Tixier et al. 2003, 2016). It was also already reported by McMurtry and Bounfour (1989) on Olea europea L. ( Oleaceae ).

Specimens examined — 4 ♀♀ + 2 ♂♂ on citrus leaves at Nour orchard in July 2018 ; 2

♀♀ on Rhamnus spp. L. ( Rhamnaceae ) and 2 ♀♀ on Zyziphus lotus L. ( Rhamnaceae ) in April 2019 at Road to Alma village in the argan forest.

Remarks — The measurements of adult females fit well with those provided by Tixier et al. (2016) for specimens from Morocco. The measurements of adult females agree with those provided by Tixier et al. (2010) for specimens from Croatia on Cornus sanguinea L. ( Cornaceae ) and by Tixier et al. (2016) for specimens from Morocco.

Identification key to females of recorded species of Moroccan Phytoseiidae

This key contains the 59 species presently recorded from Morocco.

1. Podonotal region of the dorsal shield (anterior to setae R1) of the female with five or six pairs of “lateral” setae j3, z2, z4 and s4 always present and z3 and/or s6 present............ 2 — Podonotal region of the dorsal shield (anterior to setae R1) of the female with four pairs of “lateral” setae j3, z2, z4 and s4 present, z3 and s6 absent......... Sub-family Amblyseiinae : 3

2. Posterior “lateral” setae Z1, S2, S4 and S5 absent. Setae r3 usually inserted on the dorsal shield...................................... Sub-family Phytoseiinae : Phytoseius finitimus — At least one of setae Z1, S2, S4 and S5 present. Setae r3 usually inserted on the interscutal soft cuticule (rarely on the shield).......................... Sub-family Typhlodominae: 36

3. Sternal shield with median posterior projection, some forward “migration” of pre-anal setae ZV2 and/or JV2 ...................................................................... 4 — Sternal shield without posterior projection, without forward “migration” of pre-anal setae ZV2 and/or JV2 ......................................................................8

4. Heavily sclerotised and brown body with separate anal shield and subrectangular ventral shield............................................................. Iphiseius degenerans

— Slightly sclerotised and ventri-anal shield entire........................Genus Euseius : 5

5. Cervix of the spermatheca tubular................................................... 6 — Cervix of the spermatheca of a different shape........................................ 7

6. Peritreme long, extending to level of j3 or between j3 and z2. stIV relatively short (54 μm). Cervix of spermatheca tubular with a long cervix of 20 to 25 μm long...... Euseius stipulatus — Peritreme short, extending at maximum to level of z2. Dorsal setae long. stIV long (77 μm). Cervix of spermatheca tubular, thin, long and sinuous (43 μm).............. Euseius scutalis

7. All setae very short, peritreme extending to the level of j1. Macrosetae of the leg IV sharp-tipped. Distal half of the cervix thick-walled with the one side curved distally to house the atrium................................................................ Euseius batus — setae z2, z4 and s4 long, peritreme extending to the level of j3, macrosetae of the leg IV knobbed, stIV very long. Spermatheca with broad cervix, about twice as long, as wide and slightly narrowing....................................................... Euseius dossei

8. Setae S4 absent.................................................................... 9 — Setae S4 present.................................................................. 12

9. Setae J2, S2 absent, female ventri-anal shield reduced, setae j6 very long: 2–3 times longer than distance between their bases, female ventri-anal shield with one pair of pre-anal setae...

................................................................. Phytoseiulus persimilis — Setae J2, S2 present, female ventri-anal shield elongated with three pre-anal setae, setae j6 not long............................................................................10

10. Striated dorsal shield, ZV3 absent..................................... Eharius chergui — Not striated dorsal shield, ZV3 present.............................................. 11

11. Presence of six solenostomes on the dorsal shield (gd1, gd2, gd4, gd6, gd8, gd9); ventri-anal shield reduced with two pairs of pre-anal setae.................. Kampimodromus hmiminai — Presence of four solenostomes on the dorsal shield (gd1, gd2, gd6, gd9); ventri-anal shield not reduced, elongated with threepairs of pre-anal setae........... Kampimodromus aberrans

12. Z2 present, ventri-anal shield reduced with one pair of pre-anal seta Typhloseiella isotricha — Z2 absent, ventri-anal shield not reduced............................................ 13

13. Absence of J2 ...................................................................14 — Presence of J2 ................................................................... 15

14. Differences in the length of the setae Z5 = 170–196, Z4 = 155.... Proprioseiopsis messor — Differences in the length of the setae Z5 = 203–225, Z4 = 125–127......................

............................................................. Proprioseiopsis bordjelaini

15. Macrosetae usually present only on leg IV (rarely missing on this leg) but sometimes also on leg III. Lateral dorsal setae except Z5 usually subequal. J2, Z1, S2, S4 and S5 always present .... ... ... ... .... ... ... ... .... ... ... ... ... .... ... ... ... .... ... ... ... ... .... ... ... ...16 — Macrosetae usually present on legs II, III and IV, and sometimes also on leg I. Lateral dorsal setae often of quite differentlengths. J2, Z1, S2, S4 or S5 may be missing................. 28

16. Ventri-anal shield reduced and/or markedly wider at anus level, with a marked waist................................................................. Genus Paragigagnathus : 17 — Female ventri-anal shield not reduced and/or markedly wider at anus level, without a marked waist............................................................. Genus Neoseiulus : 18

17. Seta st3 inserted on sternal shield of female................. Paragigagnathus tamaricis – Seta st3 inserted off sternal shield of female.................... Paragigagnathus molestus

18. Absence of ZV3, dorsal shield longitudinally striated............. Neoseiulus . thymeleae — Presence of ZV3, dorsal shield not longitudinally striated............................. 19

19. Spermatheca with atrium forked for at least half its length at juncture with major duct, or atrium appearing thick-walled, vacuolated............................................. 20

— Spermatheca with atrium not deeply forked at juncture with major duct, nor appearing thick-walled, vacuolated............................................................. 21

20. Punctuated pores on the ventri-anal shield.................................. N. alpinus — Crescenticpores on the ventri-anal shield........................ N. barkeri & N. stolidus

NB: impossible to distinguish between these two latter species already suspected to be synonyms by Ragusa & Athias-Henriot 1983

21. Spermatheca with elongated cervix................................................22 — Spermatheca with bell shape cervix................................................ 23

22. Very elongated cervix (38 µm)..................................... N. pseudotauricus — Not very elongated cervix......................................................... 24

23. Non crescentic pores on the ventrianal shield....................................... 24 — Crescentic pores on the ventrianal shield............................................ 25

24. Z5 = 47, Z4 =38, j3 = 22, s4 =27........................................ N. cucumeris — Z5 = 60–75, Z4 = 23–28, j3 = 18–21, s4 = 23–26......................... N. longilaterus

25. Crescenticpores on the ventri-anal shield............................... N. californicus — Minute pores on the ventri-anal shield.............................................. 26

26. Z4 = 75, j3 = 45–50, s4 = 45–60........................................... N. ornatus — Z4 = 32, j3 =17, Z5 = 42–50, s4 = 27–30............................................27

27. Z4 = 32, j3 =17, Z5 = 42–50, s4 = 27–30................................... N. marinus — Z4 = 53, j3 =38, Z5 =64, s4 = 52........................................ N. umbraticus

28. Ratio seta s4 / S2 <2.7:1.......................................................... 29 — Ratio seta s4 / S2> 3.0:1.0......................................................... 30

29. Seta S5 present.................................................... Transeius audeae — Seta S5 absent.................................................... Amblyseiella setosa

30. Spermatheca with atrium bifurcate/vacuolated at juncture with major duct...............

................................................................. Graminaseius graminis — Spermateca with atrium not bifurcate/vacuolated at the juncture with major duct..........

..................................................................Genus Amblyseius : 31

31. Setae ZV2 off the ventri-anal shield........................................ A. italicus — Setae ZV2 on the ventri-anal shield................................................. 32 32. Ventri-anal pores punctiform..................................................... 33 — Ventri-anal pores crescentic....................................................... 34

33. Setae Z5 315; Z4 162..................................................... A. obtusus — Setae Z5 180–210; Z4 110–130......................................... A. meridionalis

34. Female ventrianal shield vase-shaped, wider at level of anus than at level of setae ZV2 present........................................................... Amblyseius largoensis — Female ventrianal shied not vase-shaped, not wider at level of anus than at level of setae ZV2............................................................................... 35

35. Spermatheca with short neck; seta Z5, around 145 µm long................ A. andersoni — Spermatheca with calyx cup-shaped; setae Z5, maximum 100-120 µm long..... A. swirskii

36. Setae z3 absent................................................... Cydnoseius negevi — Seta z3 present................................................................... 37

37. Setae z6 present................................................................. 38 — Setae z6 absent................................................................... 41

38. JV3 Present...................................................... Kuzinellus saharae — JV3 absent....................................................................... 39

39. Seta Z3 present.................................................... Paraseiulus talbii — Seta Z3 absent................................................................... 40

40. Genu II 1 2/0, 2/0 1; light vertical reticulation on dorsal shield, dorsal shield elongated...

................................................................... Paraseiulus minutus — Genu II 2 2/1, 2/0 1; distinct to strong squarish reticulation on dorsal shield, rounded shape of the dorsal shield................................................. Paraseiulus triporus

41. Setae Z1 absent............................................. Genus Typhlodromus : 42 — Setae Z1 present..................................................................58

42. Seta S5 present....................................... Typhlodromus (Anthoseius) : 43 — Seta S5 absent...................................... Typhlodromus (Typhlodromus) : 49

43. Two pairs of setae on the ventri-anal shield........................ T. (Anthoseius) ilicis — Four pairs of setae on the ventri-anal shield......................................... 44

44. Presence of three pairs (gd2, gd6, gd9) of solenostomes on a strongly reticulated dorsal shield.......................................................... Typhlodromus (A.) recki — Presence five pairs of solenostomes on a less reticulate dorsal shield................... 45

45. Ventri-anal shield without pre-anal pores...........................................46 —- Ventri-anal shield with pre-anal pores................... Typhlodromus (A.) rhenanoides

46. Spermatheca with cylindrical calyx with a neck between atrium and cervix, three macrosetae on leg IV....................................................................... 47 — Spermatheca without a neck between atrium and cervix and a long cylindrical major duct, 1 macrosetae on the leg IV (on the basitarsus)........................................... 48 47. Seta j1 23–34, peritreme reaching j1 ......................... Typhlodromus (A.) foenilis — Seta j1 shorter 14–22, peritreme extending between j1 and j3 ............................ ....................................................... Typhlodromus (A.) maspalomensis

48. Peritreme extending between j1 and j3, elongated and very thin metapodal plate 1................................................................ Typhlodromus (A.) clairathiasae — Peritreme extending between j1 and j3, not so elongated and thin metapodal plate 1.................................................................... Typhlodromus (A.) athenas

49. Three solenostomes on the dorsal shield........................................... 50 — More than three solenostomes on the dorsal shield................................... 53

50. Three pairs of setae on the ventri-anal shield........................... T. (T.) thuriferus — Four pairs of setae on the ventri-anal shield......................................... 51

51. Seven setae on the genu II........................................................52 — Eight setae on the genu II.............................................. T. (T.) ballotae

52. Z4 length 27 (21–33), Z5 length 40 (32–48)............................ T. (T.) baccettii — Z4 length 43 (40–48), Z5 length 61 (53–68)............................... T. (T.) leclanti

53. Six setae on the genu II.......................................................... 54 — More than six setae on the genu II.................................................. 55

54. Peritreme extending to z2 .................................. Typhlodromus (T.) setubali — Peritreme extending to j3 .............................. Typhlodromus (T.) moroccoensis

55. Genu II with eight setae.......................................................... 56 — Genu II with seven setae.......................................................... 57

56. Z4 length 38 (30–46), Z5 length 59 (49–69), stIV length 33 (26–40)....................

........................................................ Typhlodromus (T.) octogenipilus — Z4 length 70 (65–75), Z5 length 91 (85–95), stIV length 45 (40–48).....................

.............................................................. Typhlodromus (T.) mazarii

57. Calyx of the spermatheca squared basally, with a short neck...................................................................................... Typhlodromus (T.) exhilarates — Calyx of the spermatheca rounded basally, without neck...... Typhlodromus (T.) phialatus

58. No thick setae not araising from tubercules, six small round solenostomes on the dorsal shield (gd1, gd2, gd5, gd6, gd8, gd9)................................. Neoseiulella litoralis — Thick setae raising from tubercules............................. Typhloseiulus eleonorae