Fructilactobacillus, Zheng & Wittouck & Salvetti & Franz & Harris & Mattarelli & O’Toole & Pot & Vandamme & Walter & Watanabe & Wuyts & Felis & Gänzle & Lebeer, 2020

Zheng, Jinshui, Wittouck, Stijn, Salvetti, Elisa, Franz, Charles M. A. P., Harris, Hugh M. B., Mattarelli, Paola, O’Toole, Paul W., Pot, Bruno, Vandamme, Peter, Walter, Jens, Watanabe, Koichi, Wuyts, Sander, Felis, Giovanna E., Gänzle, Michael G. & Lebeer, Sarah, 2020, A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae, International Journal of Systematic and Evolutionary Microbiology 70, pp. 2782-2858 : 2840

DESCRIPTIONOF FRUCTILACTOBACILLUS GEN. NOV.

Fructilactobacillus (Fruc.ti.lac.to.ba.cil’lus. L. masc. n. fructus, fruit; N.L. masc. n. Lactobacillus a bacterial genus name; N.L. masc. n. Fructilactobacillus , fruit-loving lactobacillus, referring to the preference for growth in presence of fructose as electron acceptor).

Gram-positive, rod-shaped, catalase negative, heterofermentative and aerotolerant. Growth is observed at 15 °C but not at 37 °C. Strains in thegenus have very small genomes ranging from 1.23 Mbp for Fructilactobacillus sanfranciscensis to 1.44 Mbp for Fructilactobacillus lindneri ; the mol% G+C content of DNA as calculated from whole genome shotgun sequences ranges from 34.1 to 38.9. Comparable to other insect-associated lactobacilli, strains in the genus ferment only few carbohydrates; some strains ferment only maltose and sucrose. Most strains of the species are fructophilic and use fructoseas electron acceptor ratherthan as carbon source. Several species in the genus are core member of insect intestinal microbiota, and were isolated from insects, flowers, and spoiled or fermented foods.

Aphylogenetic tree on the basis of 16S rRNA genes of all species in thegenus Fructilactobacillus is provided in Fig. S6Q .

The typespecies of the genus is Fructilactobacillusfructivorans comb. nov.; Fructilactobacillus was previously referred to as L. fructivorans group.

Figures

Fig. 6. Gene family presence/absence patterns in Lactobacillaceae and Leuconostocaceae. Each column represents a gene family presence/absence pattern in species of Lactobacillaceae and Leuconostoaceae, where presence is indicated with a dot. The absolute number of gene families that conform to each pattern is visualized in the marginal bar plot at the top. Separations between phylogroups are indicated with horizontal black lines. We defined genes that were present in all genomes of a clade and in none of the genomes outside of that clade as ‘signature genes’ (dark blue); other genes are shown in light blue. Only presence/absence patterns followed by four or more gene families are shown. Patterns of presence in a single species or all species are not shown. Unassigned species are clusters of closely related genomes which could not be assigned to a known species due to low whole-genome similarity to a type strain and/or low 16S rRNA similarity to a type strain.