Euscorpius kinzelbachi Tropea, Fet, Parmakelis, Kotsakiozi et Stathi, 2014

Tropea, Gioele, Fet, Victor, Parmakelis, Aristeidis, Kotsakiozi, Panayiota & Stathi, Iasmi, 2014, Three new species of Euscorpius (Scorpiones: Euscorpiidae) from Greece, Euscorpius 190 (190), pp. 1-22 : 8-12

Subgenus Euscorpius Thorell, 1876

Euscorpius kinzelbachi Tropea, Fet, Parmakelis, Kotsakiozi et Stathi , sp. nov. ( Figs. 20–38; Table 1) http://zoobank.org/ urn:lsid:zoobank.org:act:8788DF49-45E2-46E4-A5BB-3D5EF95A0F7A

REFERENCES:

Euscorpius carpathicus s.str. (in part; Mt. Olympus): Kinzelbach, 1975: 30, 36

Euscorpius carpathicus (in part; Mt. Olympus): Kinzelbach, 1982: 60–61; Stathi & Mylonas, 2001: 289.

Euscorpius carpathicus “Subgroup A 1” (in part; Mt. Olympus): Fet, 2000: 52.

Euscorpius ossae (Clade E9, in part; Mt. Olympus): Parmakelis et al., 2013: 740.

Type material (33 specimens: 7 ♂, 26 ♀): Holotype: ♂, GREECE. Thessaly: Mt. Olympus, around Naoum Cave , pine forest, 1375 m, 40.10329819°N, 22.32180023°E, 3 June 2007, leg. M. Mylonas ( NHMC 10053 ; 81.1.1.61, Eus 2) . Paratypes: GREECE. Thessaly: same label as holotype, 2 ♂ (1 adult and 1 imm.), 9 ♀ (8 adults and 1 imm.) ( NHMC 10053 , 81.1 .1.61, Eus 2), 1 ♂, 2 ♀ ( GTC) ; Kokkinopilos, 4.2 km to Tsoureka spring, 1200 m, 4 June 2007, leg. M. Mylonas, 1 ♂, 5 ♀ ( NHMC 10056 , Eus 1), 1 ♂, 1 ♀ ( GTC) ; Mt. Olympus, 2100 m, near refuge of Alpine Club [“Spilios Agapitos”], leg. Mission A. Bertrand, 14 June 1955, 1 ♀ ( MNHNP RS 2970 ) ; Mt. Olympus , 1800 m, July 1972, leg. A. Villiers, 1 ♂ ( MNHNP RS 7686 ) ; Mt. Olympus , near Prionia, 1500 m, 1 ♀ ( FKPC) . Central Macedonia: Mt. Olympus, foothills, Litochoro , July 1997, leg. M. Švarc, 1 ♂ ( FKPC) .

Other material examined (not included in type series) (29 specimens: 12 ♂, 12 ♀, 7 juv): GREECE. Thessaly: Mt. Olympus , 2100 m, 3 July 1936, leg. Kr. Tuleshkov ,

1 ♂ ( NMHNS); Mt. Olympus , 2000 m, 6 July 1936, leg. D. Papazov , 7 juv. ( NMNHS) ; 1 ♂, Mt. Olympus , 1700 m, 17 September 1974, leg. P. Beron & V. Beshkov ( NMHNS 55 ); Mt. Olympus , east portion, 14 June 1973, leg. H. Schmalfuss , 1 ♂, 1 ♀ ( ZMM 0199 a); Mt. Olympus, Agios Dionysios Monastery , 820 m, 28 August and 10 September 1973, leg. H. Pieper , 1 ♀ ( ZMM 0209 ); Mt. Olympus, Karia , 800 m, 26 May 1974, leg. H. Malicky , 1 ♂ imm., 1 ♀ ( ZMM 0261 ); SMt. Olympus, 1200 m, Oxias Forest , 4 June 2007, leg. M. Mylonas , 2 ♂, 6 ♀, 2 juv. ( NHMC 10056 , 81.1 .1.52-54); Mt. Olympus, 1305 m, E from Kokkinopilos , 10 June 2003 – 23 June 2004, leg. P.M. Giachino & D. Vailati , 1 ♂ imm. ( MSNG, PMGC Sc 71); Mt. Olympos, Kokkinopilos , 1070 m, groove in beech forest, 10 June 2003 – 23 June 2004, leg. P.M. Giachino & D. Vailati , 1 ♂ imm. ( MSNG, PMGC Sc 72); Mt. Olympus , near Prionia, 1500 m , 1♂, 4♀ ( FKPC). Central Macedonia: Mt. Olympus, foothills, Litochoro , July 1997, leg. M. Švarc , 1 ♂ ( FKPC); Mt. Olympus , foothills, 3 km N Litochoro, 15 May 2001 , leg. V. Fet , 2 ♀ ( VFPC) .

DNA sequences ( Parmakelis et al., 2013: 740): GREECE. Thessaly: Mt. Olympus, Naoum Cave , 1375 m, 40.10329819°N, 22.32180023°E, 3 June 2007, leg. M. Mylonas ( NHMC 10053 , Eus2), EC212, geneseq-1: 16S, COI, COII, ITS1 (GenBank accession numbers KC215615 , KC215699 , KC215784 , KC215870 ) ; Mt. Olympus, Kokkinopilos, 4.2 km to Tsoureka spring, 1200 m, 4 June 2007, leg. M. Mylonas ( NHMC 10056 , Eus1), EC205, geneseq-2: 16S, COI, COII, ITS1 (Gen- Bank accession numbers KC215610 , KC215694 , KC215 779, KC215865 ) .

Etymology. Named after our colleague, Prof. Ragnar Kinzelbach (Rostock, Germany), whose research greatly contributed to the current knowledge of Aegean and Anatolian scorpions.

Geographic range. Known only from Mt. Olympus massif, Thessaly and Macedonia, Greece ( Fig. 54).

Diagnosis: A medium to medium-large Euscorpius species , usually 33–35 mm long (range 30–39 mm, mean 34.7 mm). Color of adults medium-light to medium-dark brown, with carapace darker. Weak to marked reticulation or marbling on chelicerae, carapace, mesosoma and metasoma is present. The number of trichobothria on the pedipalp manus ventral surface is 4 (V 1-3 +Et 1). The number of trichobothria on the pedipalp patella ventral surface usually is 8 (in 73.17 %), more rarely 7 (in 21.95 % of pedipalps examined). The number of trichobothria on pedipalp patella external surface usually is: eb = 4, eba = 4, esb = 2, em = 4, est = 4, et = 6. The pectinal teeth number in males is 8 to 10, and in females usually 7 to 8 (7 in 42.31 % and 8 in 51.92 % of the pectines examined). Lchel/Wchel ratio is 2.637 in males and 2.706 in females. Dorsal patellar spur well-developed. Femur could be slightly longer to slightly shorter than patella; Lfem/Lpat ratio is 0.996. Carapace could be either slightly longer than wide (mostly in males) or slightly wider than long (mostly in females); average ratio Lcar/Wcar 1.039 in males and 1.003 in females; average distance from center of median eyes to anterior margin of the carapace is 41.05 % of the carapace length. Average ratio of Lmet/Lcar is 2.696 in males and 2.479 in females.

Trichobothrial and pectinal teeth count variation. The variation observed in 42 studied specimens (16 ♂, 26 ♀) is given below.

Pectinal teeth in males: 8/8 (1), 8/9 (2), 9/8 (2), 9/9 (5), 10/9 (2), 10/10 (4); in total, 8 in 6 (18.75 %), 9 in 16 (50.00 %), and 10 in 10 (31.25 %); mean = 9.13, SD = 0.71 (n=32).

Pectinal teeth in females: 6/7 (1), 7/7 (7), 7/8 (4), 8/7 (3), 8/8 (10), 9/9 (1); in total, 6 in 1 (1.92 %), 7 in 22 (42.31 %), 8 in 27 (51.92 %), and 9 in 2 (3.85 %); mean = 7.58, SD = 0.61 (n=52).

Pedipalp patella trichobothria Pv:?/7 (1), 7/7 (4), 7/8 (9), 8/6 (1), 8/8 (23), 8/9 (2), 9/8 (1), 9/9 (1); in total, 6 in 1 (1.22 %), 7 in 18 (21.95 %), 8 in 60 (73.17 %), and 9 in 4 (4.88 %); mean = 7.81, SD = 0.53 (n=83).

Pedipalp patella trichobothria Pe: et = 5/5 (2), 5/6 (2), 6/4 (1),?/6 (1), 6/6 (34), 6/7 (2), in total, 4 in 1 (1.20 %), 5 in 6 (7.23 %), 6 in 74 (89.16 %), and 7 in 2 (2.41 %); mean = 5.93, SD = 0.36 (n=83); est =?/4 (1), 4/3 (1), 4/4 (40), 5/4 (1); em =?/4 (1), 2/4 (1), 4/4 (40); esb =?/2 (1), 2/1 (1), 2/2 (39), 2/3 (1), 3/3 (1); eba =?/4 (1), 4/4 (41); eb =?/4 (1), 4/4 (41).

Hemispermatophore. Both right and left hemispermatophores of two specimens were studied. They have a well-developed lamina tapered distally; well-developed basal constriction present; truncal flexure present; median projection with primary and secondary acuminate processes; internal projection distally show 9–12 tines in its crown. The number of tines of the crown could differ between specimens and between the right and the left hemispermatophores. The shape of the secondary acuminate processes varies between specimens and between the right and the left hemispermatophores.

Description of the male holotype

Coloration: Whole color medium brown with carapace darker, brown/reddish, and legs, telson and chelicerae lighter; more or less expressed marbling on carapace, tergites, metasoma and chelicerae; sternites and pectines and genital operculum very light brownish/ivory; chelicerae very light, yellowish/light brownish with darker finger teeth, palms with slight marbling; telson yellowish, with a longitudinal darker line and dark reddish aculeus tip; all pedipalp carinae darker, dark brown to blackish colored.

Carapace: Marked granulation of variable size on whole surface is present, except in the anterior area between the anterior edge, the lateral eyes and median eyes, which is almost smooth. The lateral area behind the lateral eyes has greater and marked granules; anterior edge granulate and concave; median anterior, median posterior, and posterior lateral furrows well evident; two pairs of lateral eyes (with a larger anterior eye), and a pair of median eyes, situated distally of the middle; distance from center of median eyes to anterior margin is 41.66% of carapace length.

Mesosoma: Tergites finely granulated; sternites glossy and finely punctuated. Small spiracles inclined about 45° downward towards outside.

Metasoma: Dorsal carinae on segments I–IV with little spaced granules, with the last two distal granules well-marked on segments II–IV; dorsolateral carinae on segments I barely traceable with very few, small, spaced granules, in the proximal area, on segments II–V absent or obsolete; ventrolateral carinae absent on segment I, obsolete or smooth on segments II–IV, granulated to serrulated on segment V; ventromedian carina absent on segments I–IV, granulated on segment V; dorsal intercarinal spaces with a very fine granulation, from smooth to very fine and scattered granules on the lateral and ventral surface.

Telson: Vesicle with a few scattered, very small granules, with ventral setae of different size, especially near the vesicle/aculeus juncture.

Pectines: Teeth number 9/9; middle lamellae number 5/5; several microsetae on proximal area of teeth, marginal lamellae, middle lamellae and fulcra.

Genital operculum: The genital operculum is formed by two longitudinally separate subtriangular sclerites; genital papillae protruding; a few microsetae are present.

Sternum: Pentagonal shape, type 2; slightly wider than long, with a deep posterior emargination.

Pedipalps: Coxa and trochanter with tuberculated carinae. Femur: dorsal and ventral internal carinae tuberculated; dorsal external carinae formed by tubercles slightly spaced and serrulated; external median carinae serrulated; ventral external carinae formed by spaced tubercles, well-formed only in the proximal 1/3, then it merges with the surrounding granulation; anterior median formed by spaced 8–10 conical tubercles, varying in size, of which three are greater and wellmarked with a macroseta each; dorsal and ventral intercarinal spaces with granules of variable size, greater near the carinae. Patella: dorsal and ventral internal carinae tuberculated to granulated; dorsal external carinae from rough to slightly crenulated; ventral external carinae from granulated, slightly serrulated; dorsal intercarinal surface from almost smooth to granulated with granules of variable size, greater near the internal carinae and in distal area; ventral intercarinal surface most smooth with a few scattered small granules, especially near ventral internal carinae. Dorsal patellar spur well developed. Chelal carina D1 and V1 are distinct, strong, dark and mostly smooth with a few granules proximally; D4 and V3 rounded, dark, lightly and finely granulated; external carina granulated; intercarinal tegument rough with granules of variable size. Typical Euscorpius chela finger dentition.

Trichobothria: Chela: trichobothria on the pedipalp manus ventral surface 4/4 (V 1-3 +Et 1). Patella ventral (Pv): 7/8. Patella external (Pe): et = 6/6, est = 4/4, em = 4/4, esb = 2/2, eba = 4/4, eb = 4 / 4. Femur: trichobothrium d is slightly proximal to i, while trichobothrium e is distal to both d and i, and situated on dorsal external carina.

Legs: With two pedal spurs; no tarsal spur; ventral row of tarsus III with a total of 12/14 spinules, all on a single line; 3 flanking pairs of tarsal setae adjacent to the ventral spinules row. Basitarsus with from 2 to 10 prolateral spinules, of increasing number from legs IV to I. Granulation present on dorsal and ventral surface of leg femora, it is mostly marked and dark ventrally (except the right femur of leg II, which is slightly deformed).

Chelicerae: Movable finger: the dorsal distal denticle is very smaller than the ventral distal denticle; ventral edge is smooth with brush-like setae on the inner part; dorsal edge has five denticles: one large distal, two small subdistal, one large median and a small basal. Fixed finger: it has four denticles: one distal, one subdistal, one median and one basal, the last two in a fork arrangement; the internal surface has brush-like setae.