Macrocamptoptera setata Triapitsynı 2012

Triapitsyn, Serguei V., Dominguez, Chrysalyn, Huber, John T., Japoshvili, George & Heraty, John M., 2020, Morphological and molecular separation between Macrocamptoptera grangeri Soyka and M. metotarsa (Girault) (Hymenoptera: Mymaridae), Journal of Natural History 54 (9), pp. 585-596 : 595

Macrocamptoptera setata Triapitsynı 2012

Macrocamptoptera setata Triapitsyn 2012: 104 – 105 . Type locality: Oak Glen, San Bernardino County, California, USA. Holotype female [UCRC], examined ( Triapitsyn 2012).

Material examined

USA: Arizona, Graham Co. , Mt. Graham campground , 2040 m, 32°38.90 ʹ N 109°49.03 ʹ W, 14 – 21.viii.2015, M. E. Irwin [1 female, CNC] . California, San Diego Co ., Camp Ole , Los Huecos Road, 32°52 ʹ 53 ʹ ’ N 116°25 ʹ 51 ʹ ’ W, 1750 m, 16 – 23.viii.2011, V. Lopez [1 female, UCRC] .

Distribution

Nearctic Region : USA (Arizona [new record], California and Oregon) ( Triapitsyn 2012) .

Molecular analysis and a brief discussion

Based on our combined molecular analysis there is high support of Macrocamptoptera grangeri and M. sundhomi to be genetically identical species ( Figure 5 ). There were no sequence variations differences observed in the mitochondrial COI barcode and NJ regions as well as the ribosomal 18S, 28s D2 regions (GenBank accessions for COI NJ MT484336 – MT484338 , for COI Barcode MT498837 – MT498839 , for 18S MT498691 – MT498693 , and for 28S D2 MT498688 – MT498690 ). Macrocamptoptera grangeri and M. sundhomi differed greatly from M. metotarsa by 62 base pairs (bp) in COI barcode, 27 bp in COI NJ, 5 bp in 18S, and 30 bp in 28s D2 which corroborated their separation from the strictly Nearctic M. metotarsa . Thus, based on the presented morphological and high genetic variations found in mitochondrial COI barcode, COI NJ, and ribosomal 28S D2 region, only one species of Macrocamptopteraı M. grangeri , is currently recognised in the Palaearctic region, with M. sundholmi and M. bulgarica proposed herein as its synonyms.

Figures

Figure 5. Maximum likelihood phylogeny elucidating relationships of the three nominal Macrocamptoptera species based on the combined molecular analysis of mitochondrial DNA COI barcode, COI NJ as well as 18S, 28S nuclear ribosomal gene regions. Numbers associated with branches indicates measured support values above 50 of 1000 bootstrap replicates. Species are associated with molecular voucher D numbers of J. M. Heraty Laboratory.