Procyrnea javaensis, Zhang, Luping, Dewi, Kartika & Purwaningsih, Endang, 2009

Zhang, Luping, Dewi, Kartika & Purwaningsih, Endang, 2009, Two new species of habronematid nematodes (Nematoda: Spirurida: Habronematidae) in birds of prey from West Java, Indonesia with a key to species of Torquatoides, Zootaxa 2290, pp. 50-58 : 51-52

Procyrnea javaensis n. sp.

( Figs. 1–16 )

General description. Body of medium size, slender, with numerous, fine transverse striations. Lip region continuous with body; no constriction present. Labial region consisting of dorsal and ventral pseudolabia. Pseudolabia narrow in mid-region, expanded slightly at base and greatly expanded distally; amphids located on base of pseudolabia; three small teeth on interior border of each pseudolabium, other papillae not observed. Dorsal and ventral labia each consisting of two submedian lobes, with one large papillae on each, and median internal process. Lateral alae absent. Buccal cavity short with thick, sclerotized lining. Oesophagus divided into short anterior muscular part and long posterior glandular part: muscular part 4.0–5% of total body length (TBL), glandular part 26–36% of TBL. Nerve ring located at middle of muscular oesophagus; cervical papillae anterior to nerve ring. Excretory pore immediately posterior to nerve ring.

Male (n= 3). Body length 7.44–8.09 mm, maximum diameter 240–288 µm. Head diameter 32–35 µm. Buccal cavity 50–60 µm long, 20–30 µm diameter. Nerve ring 150–190 µm from anterior end; excretory pore 220–260 µm from anterior end; cervical papillae 110–130 µm from anterior end. Muscular part of oesophagus 325–340 µm long, 50–70 µm wide; glandular oesophagus 2.54–3.12 mm long, 130–150 µm wide. Distance between striations at mid-body 5.6–6.1 µm. Posterior end of body curved without ridges. Caudal alae welldeveloped, with longitudinal striations on ventral surface and transverse striations on dorsal surface, asymmetrical; left relatively short and narrow, 540–660 µm long, 60–70 µm wide; right slightly longer and wider, 1000–1050 µm long, 95–120 µm wide. Ventral surface of caudal region with prominent, longitudinal ridges originating near posterior end, extending up to 700 µm anteriorly. Four pairs of pedunculated preanal papillae arranged symmetrically; one large, median, pedunculated papilla anterior to cloaca; two pairs of pedunculated postanal papillae arranged asymmetrically; three pairs sessile papillae near tail tip. Tail bluntly rounded, 190–210 µm long. Spicules unequal and dissimilar; left long slender, 1.35–1.63 mm long, with pointed distal end; right short and thick, 310–450 µm long, with tip of distal end expanded slightly. Ratio of right spicule to left spicule lengths 1:3.62–4.35. Gubernaculum linguiform, 45–57 µm long.

Female (n=2). Body length 8.63–10.81 mm; maximum diameter 242–385 µm. Head diameter 35–40 µm. Buccal cavity 50–64 µm long, 30–35 µm diameter. Nerve ring 210µm from anterior end; excretory pore 330– 340 µm from anterior end; cervical papillae 130–160 µm from anterior end. Muscular part of oesophagus 330–420 µm long, 70µm wide; glandular part of oesophagus 2.43–2.87 mm long, 140–170 µm diameter. Vulva opening at ventral center post-equatorial, 3.37–4.54 mm from posterior end. Tail bluntly rounded and bent ventrally, 140–161 µm long. Eggs ellipsoidal, thick shelled, 35–45 µm long, 15–23 µm wide, unembryonated in uteri.

Host. Otus bakkamoena lempiji (Horsfield, 1821) (Aves: Strigiformes : Strigidae )

Site of infection. Under the lining of the gizzard.

Prevalence. 1/1

Intensity. 3 males and 2 females.

Type locality. Jalur Apai, Gunung Ciremai National Park, West Java, Indonesia (6°55’S, 108° 22’E, elevation 1,700m)

Type specimens. Holotype, one male ( MZB Na 403); allotype, one female ( MZB Na 404); paratypes, 2 males and 1 female ( MZB Na 405)

Etymology. The new species is named based on its occurrence in West Java, Indonesia.

Remarks. Procyrnea javaensis n. sp. resembles P. f i c h e u r i (Seurat, 1916), P. m u r r a y i (Ortlepp, 1934), P.a m e e r a e ( Ali, 1961), P. dollfusi ( Mawson, 1968) , P. a p t e r a ( Wang, 1976), and P. brevicaudata Zhang, Brooks and Causey, 2004 , in lacking lateral alae. P. brevicaudata can be distinguished from the new species because it has two longitudinal ridges on the left side of the body (ridges absent in P. javaensis n. sp.), a sinistral vulvar opening (opening at ventral center in P. javaensis n. sp.), and a dorsally bent female tail (ventrally bent in P. javaensis n. sp.). The new species differs from P. ficheuri in having a left spicule with a pointed rather than double-barbed tip, and in having asymmetrical caudal alae instead of symmetrical caudal alae. Procyrnea javaensis n. sp. can be distinguished from P. m u r r a y i by having a left spicule with a pointed tip versus a single barbed tip, and a longer left spicule (1.35–1.63 mm in the new species versus 0.88–0.89 mm in P. murrayi ). The new species differs from P. ameerae by having a longer left spicule (1.36–1.63 mm versus 0.78–0.89 mm), a different ratio of spicule lengths (1: 3.62–4.35 in P. javaensis versus 1: 3 in P. ameerae ), and a single median preanal papilla (versus none in P. ameerae ). The new species differs from P. dollfusi and P. aptera in having a different ratio of spicule lengths (1: 3.62–4.35 in P. javaensis versus 1: 2.4– 3.2 in P. dollfusi and 1: 2.4 in P. a p t e r a), and in having a shorter female tail (140–161 µm long in P. javaensis versus 320–390 µm in P. dollfusi and 240–260 µm in P. a p t e r a). The new species also differs from P. dollfusi in having a pointed tip to the left spicule instead of a single barbed tip. A new species named Habronema (Aviabronema) haliasturi Gupta and Kumar, 1980 according to Ali’s classification (see Ali, 1961), was collected from Haliastur indus (Boddaert) in India. However, the subgenus Aviabronema was regarded as a synonym of Procyrnea by Chabaud (1975). As a result, this species should be moved into the genus Procyrnea , forming a new combination, Procyrnea haliasturi ( Gupta and Kumar, 1980) n. comb. The new species differs from P. haliasturi in having a ratio of spicule lengths of 1:3.62–4.35 rather than 1:2.8, and in having a post-equatorial rather than pre-equatorial vulva.

Figures

FIGURES 1 – 10. Procyrnea javaensis n. sp. 1. Cephalic region of male, ventro-lateral view; 2. Apical view, showing mouth opening and internal teeth; 3. Transverse striations in mid-body region, dorsal view; 4. Cervical papilla; 5. Posterior end of female, ventral view; 6. Posterior end of male, ventral view; 7. Posterior end of male showing end of longitudinal ridges on ventral surface; 8. A single median preanal papilla, showing club-shaped papilla; 9. Mushroomshaped caudal papilla; 10. Spherical caudal papilla. Scale bars: Figs. 1, 3, 10 µm; Figs. 2, 4, 8, 9, 5 µm; Figs. 5, 7, 50 µm; Figs. 6, 200 µm.

FIGURES 11 – 16. Procyrnea javaensis n. sp. 11. Anterior end of male, lateral view; 12. Posterior end of male, ventral view; 13. Gubernaculum, ventral view; 14. Anterior extremity of right spicule; 15. Posterior end of female, lateral view; 16. Eggs. Scale bars: figs. 11, 14, 15, 50 µm; 12, 100 µm; figs. 13, 16, 25 µm.