Paracrassiperidium Jayasiri, Tibpromma & Karun., 2024

Jayasiri, Subashini Chathumini, Tibpromma, Saowaluck, Xu, Ruifang, Bhat, Darbhe Jayarama, Elgorban, Abdallah M., Karunarathna, Samantha C., Rampelotto, Pabulo H., Han, Meiyan & Xu, Jianchu, 2024, Unveiling new fungal discoveries in Yunnan Province, China, Phytotaxa 676 (2), pp. 103-125 : 115

Paracrassiperidium Jayasiri, Tibpromma & Karun. , gen. nov.

Index Fungorum number: IF903019

Etymology: The epithet “ Paracrassiperidium ” refers to the morphological similarity of the new genus to Crassiperidium .

Type species: Paracrassiperidium fusiforme Jayasiri, Tibpromma & Karun.

Saprobic on the branches, leaves, wood, or twigs in terrestrial habitat. Sexual morph: Ascomata semi-immersed to immersed, dome-shaped, scattered, subglobose, uni-loculate, with or without a papillate ostiole. Peridium unequal thickness, composed of several layers of brown to dark brown cells, arranged in textura angularis. Hamathecium filamentous, branched, septate, pseudoparaphyses, anastomosed between the asci, embedded in a hyaline gelatinous matrix. Asci 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, pedicellate, with well-developed ocular chamber. Ascospores fusiform, hyaline to light brown, 1–5 septate, constricted at the septa, the upper cell larger than the lower cell, conical at both ends, guttulate, smooth-walled, with a mucilaginous sheath. Asexual morph: Undetermined.

Notes: This study introduces Paracrassiperidium (type species: Paracrassiperidium fusiforme ) as a new genus. Paracrassiperidium , with its perithecial ascomata, bitunicate asci, and hyaline to brown ascospores, shares a strong morphological connection with members of Pleosporales .

Paracrassiperidium fusiforme (KUMCC 21-0041a and KUMCC 21-0041b) formed a distinct lineage sister to members of Crassiperidium , Shearia and Longiostiolum in Longiostiolaceae with 1.00 BYPP bootstrap support ( Figure. 5 ). However, we did not obtain more than 70% ML support for this relationship. Paracrassiperidium shares similar morphology to Crassiperidium , but Paracrassiperidium has 8-spored asci, hyaline to light brown, fusiform ascospores with 1–5-septate, while Crassiperidium has 4–8-spored asci, hyaline, broadly fusiform ascospores with 1– 3-septate ( Matsumura et al. 2018). In addition, the comparison of TEF 1 and RPB 2 sequences between our new isolate and Crassiperidium octosporum (KT 2144) shows 95/892 (10.65 %) and 169/895 (18.88 %) base pair differences, respectively. Similarly, the comparison of TEF 1 and RPB 2 sequences between our new isolate and Crassiperidium quadrisporum (KT 2798-1) shows 90/910 (9.89 %) and 169/915 (18.46 %) base pair differences, respectively.

Figures

FIGURE 5. The phylogram generated from maximum likelihood analysis based on LSU, SSU, TEF1, and RPB2 sequence data represents Longiostiolaceae and closely related families. Related sequences are obtained following Wanasinghe et al. (2020). Thirty-six strains are included in the combined analyses, with three species from the family Melanommataceae being used as the outgroup taxa. Bootstrap values for ML are equal to or greater than 70%, and clade credibility values equal to or greater than 0.95 (the rounding of values to 2 decimal proportions) from the BYPP analysis labeled on the nodes.A newly generated sequence is in blue, and type species are bold.