Pseudokeissleriella bambusicola Yi Yang, S.N. Zhang & Jian K. Liu (2022)

Jayasiri, Subashini Chathumini, Tibpromma, Saowaluck, Xu, Ruifang, Bhat, Darbhe Jayarama, Elgorban, Abdallah M., Karunarathna, Samantha C., Rampelotto, Pabulo H., Han, Meiyan & Xu, Jianchu, 2024, Unveiling new fungal discoveries in Yunnan Province, China, Phytotaxa 676 (2), pp. 103-125 : 113-114

Pseudokeissleriella bambusicola Yi Yang, S.N. Zhang & Jian K. Liu (2022) FIGURE 4

Index Fungorum number: IF844118

Saprobic on bamboo species. Sexual morph: see Yang et al. (2022). Asexual morph: Conidiomata 160–200 μm diam. (x̄ = 182 μm, n = 15), pycnidia, immersed to sub-immersed, multi-loculate, dark brown. Conidiomatal wall 20–40 μm wide (x̄ = 27 μm, n = 10), thick-walled, brown to hyaline, with 5–10 cell-layers of textura angularis. Conidiophores 4–5 μm high × 2–3 μm wide (x̄ = 4.8 × 2.7 μm, n = 10), hyaline, short, with a few conidiogenous cells arising from one conidiophore, attached to conidiomatal wall. Conidiogenous cells 3–5 μm × 2–3 μm (x̄ = 4 × 2.5 μm, n = 30), cylindrical, hyaline, phialidic, bottle-shaped, aseptate, with a minute collarette. Conidia 2–3 × 1–1.5 μm (x̄ = 2.6 × 1.3 μm, n = 30), ellipsoid, one-celled, hyaline, smooth-walled.

Culture characteristics: Conidia germinated within 12 hours on PDA, colonies grown on PDA at room temperature (20–25 °C). Colonies are circular to irregular, dull surfaces and wrinkled, white to gray, aerial hyphae at the edge. Reverse dark brown middle and yellowish-brown edge, producing a yellow-orange pigment on PDA.

Material examined: China, Yunnan Province, Kunming, Kunming Institute of Botany Botanical Garden, Chinese Academy of Science, on dead branches of bamboo species ( Poaceae ), 08 June 2020, Tibpromma S, ST 87 (HKAS 115768), living culture KUMCC 21-0058.

Known distribution: Bamboo in China ( Yang et al. 2022; this study).

Notes: Our new strain is introduced as an asexual morph of Pseudokeissleriella bambusicola , based on analysis of a combined SSU, ITS LSU, and TEF 1 dataset and nucleotide comparison. Our new strain (KUMCC 21-0058) clusters basal to the strains of Pseudokeissleriella bambusicola (CGMCC 3.20950 and UESTCC 22.0028) with 100% ML and 1.00 BYPP bootstrap support ( Figure. 3 ). The comparison of ITS and TEF 1 sequences between the new isolate and Pseudokeissleriella bambusicola (CGMCC 3.20950) shows only three (0.55%) and one (0.11%) base pair differences. The SSU and LSU genes showed no base pair differences between these two strains. Thus, we introduce our isolate as a new strain of Pseudokeissleriella bambusicola according to the guidelines of Jeewon & Hyde (2016).

Our isolate is a phoma-like asexual morph, but Pseudokeissleriella bambusicola (CGMCC 3.20950) was only introduced by its sexual morph by Yang et al. (2022). Therefore, the morphological comparison between our new strain and Pseudokeissleriella bambusicola is impossible. However, the new strain shares similar morphology with the asexual morph description of the family Lentitheciaceae in having pycnidial, dark brown to black, semi-immersed or immersed, multi-loculate, ostiolate, subglobose conidiomata without verruculose to warty setae, subcylindrical to ampulliform, sometimes proliferating conidiogenous cells with hyaline, aseptate, subglobose to subcylindrical, smooth-walled conidia ( Hyde et al. 2013; Quaedvlieg et al. 2013; Crous et al. 2014; Wanasinghe et al. 2014, 2018; Wijayawardene et al. 2015; Li et al. 2016; Tibpromma et al. 2017, 2018). Therefore, the new strain is introduced as the asexual morph of Pseudokeissleriella bambusicola , and this study further provided SEM photographs of the conidia of Pseudokeissleriella bambusicola .

Longiostiolaceae Phukhams., Doilom & K.D. Hyde (2020)

Phukhamsakda et al. (2020) introduced Longiostiolaceae to accommodate Crassiperidium and Longiostiolum (type genus). These two genera were previously assigned to Cyclothyriellaceae ( Crassiperidium ) and incertae sedis taxon in Pleosporales ( Longiostiolum ) ( Li et al. 2016; Matsumura et al. 2018). Wanasinghe et al. (2020) designated a neotype for Shearia and assigned Shearia as a member of Longiostiolaceae based on fresh collections, which was phylogenetically highly supported. Later, Madagammana et al. (2023), confirmed that Shearia belongs to the Longiostiolaceae based on their fresh collections along with multigene phylogenetic analyses. To date, three genera ( Crassiperidium , Longiostiolum , and Shearia ) have been accepted in Longiostiolaceae .

Figures

FIGURE 3. The phylogram was generated from maximum likelihood analysis based on SSU, ITS, LSU, and TEF1 sequence data representing the family Lentitheciaceae. Related sequences are obtained following Yang et al. (2022) and Tian et al. (2024). Forty-one strains are included in the combined analyses, with two Massarina spp. (CBS 473.64, CBS 266.62) were used as the outgroup. Bootstrap values for ML are equal to or greater than 70%, and clade credibility values are equal to or greater than 0.95 from the BYPP analysis labeled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.

FIGURE 4. Asexual morph of Pseudokeissleriella bambusicola (HKAS 115768). a Section through conidiomata. b Conidioma wall. c–f Conidiogenous cells. g, h Conidiospores (SEM). i Germinated spore. j Upper view on PDA. k Reverse view on PDA. Scale bars: a = 100 μm, b = 10 μm, c–f = 5 μm, g–i = 2 μm, j, p = 2 cm.