Diatrype disciformis (Hoffm.) Fr., Summa

Mehrabi, Mehdi, Hemmati, Roghayeh, Vasilyeva, Larissa N. & Trouillas, Florent P., 2016, Diatrypella macrospora sp. nov. and new records of diatrypaceous fungi from Iran, Phytotaxa 252 (1), pp. 43-55 : 53

Diatrype disciformis (Hoffm.) Fr., Summa veg. Scand., Section Post . (Stockholm): 385 (1849). Fig. 7

Basionym: Sphaeria disciformis Hoffm., Veg. Crypt. 1: 15 (1787).

Saprobic on decaying wood with bark. Sexual morph: Stromata erumpent from bark of dead branches (8 mm diameter), disc-shaped, 1.5–2 mm diam., flat or slightly convex, dark at the surface, whitish inside, base sunk to the wood and circumscribed by a black line. Ostioles dark, at the same level as the discs surface, sulcate. Perithecia 10–23 (or more) in a stroma, dark, globoid to subgloboid, 300–600 μm diam. asci elongate, clavate, 45–70 (–90) × 3.5–5(–6) μm, octospori, ascospore allantoids, hyaline, aseptate, 5–7 × 1–1.7 μm. Paraphyses were elongate, filiform. Asexual morph: Undetermined.

Cultural characteristics:—Colonies circular to slightly irregular, white on PDA, cottony, reverse of colonies primrose (23 ,, b) after 2 weeks in the dark at 24°C, covering 75 mm Petri-dish after 8 days at 24°C.

Specimen examined:— Iran, Guilan Province, Rasht City, on dead branches of Alnus sp. , 11 July 2013, M. Mehrabi, GNA14 ( IRAN 16680F, IRAN 2347C).

Notes:—This species seems to be extremely rare outside Europe and restricted to Fagus sp. ( Vasilyeva 2011, Rappaz 1987). The Iranian material examined was consistent with D. disciformis as described by Ellis & Everhart (1892). This species has also described by Senanayake et al. (2015) on Ostrya carpinifolia from Italy. The material studied here fits also with the description of Senanayake et al. (2015). The phylogenetic analyses of the ITS sequences supported the morphological identification (bootstrap value= 93% in MP and 99% in NJ, Fig. 1 ). This species was recently reported from Fagus orientalis in Iran ( Pourmoghaddam et al. 2015). The present description is the first report of this species from Alnus sp. in Iran.

Figures

FIGURE 1. One of the 48 most parsimonious trees obtained from the ITS sequence data. MP/NJ Bootstrap support values from 1000 replicates higher than 50% are reported at the nodes.The original strain numbers are noted after the species names. The ex-type strains are in bold. The new sequences obtained in this study are indicated in highlight.

FIGURE 7. Diatrype disciformis from Alnus sp. (1) Habit of ascostromata on bark. (2) Transverse section through the ascoma shows globose perithecia surrounded in white entostroma (3) Ascus with ascocpores (4) Ascospores (5) Colony morphology on PDA.—Bars 1 = 2 mm. 2 = 1 mm. 3,4= 10 μm.