Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004

Pinacho-Pinacho, Carlos Daniel, Sereno-Uribe, Ana L., León, Gerardo Pérez-Ponce De & García-Varela, Martín, 2015, Checklist of the species of Neoechinorhynchus (Acanthocephala: Neoechinorhynchidae) in fishes and turtles in Middle-America, and their delimitation based on sequences of the 28 S rDNA, Zootaxa 3985 (1), pp. 98-116: 106-111

publication ID

http://dx.doi.org/10.11646/zootaxa.3985.1.5

publication LSID

lsid:zoobank.org:pub:E6FF2FAB-4C99-4E4A-8E56-8D9B5A161474

persistent identifier

http://treatment.plazi.org/id/03919446-652F-434E-8CE0-F8FFFEA2C3A7

treatment provided by

Plazi

scientific name

Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004
status

 

Neoechinorhynchus (Neoechinorhynchus) schmidti Barger, Thatcher & Nickol, 2004 

( Fig. 2View FIGURE 2 i)

Mexico: TABASCO: 24 km to S of Villahermosa; Trachemys venusta  (Barger 2004; Barger et al. 2004). Pantanos de Centla (18 ° 28 ’ 18.9 ’’N 92 ° 39 ’ 14.9 ’’W). Trachemys scripta  ( Martínez-Aquino et al. 2009; García-Varela et al. 2011).

Specimens deposited. CNHE (6764), HWMLAbout HWML (17667), USNPCAbout USNPC (92883).

Species delimitation through phylogenetic analysis and genetic divergence. To demonstrate the species boundaries among the species reported in this checklist, we sequenced a total of 269 individuals of the species: N. (N.) brentnickoli  (81 individuals from 16 localities); N. (N.) roseum  (two individuals from two localities); N. (N.) mamesi  (22 individuals from seven localities); N. (N.) golvani  (80 individuals from 11 localities); N. (N.) mexicoensis  (47 individuals from eight localities); N. (N.) schmidti  (five individuals from one locality); N. (N.) emyditoides  (26 individuals from three localities); N. (N.) panucensis  (four individuals from two localities); and N. (N.) chimalapasensis  (two individuals from one locality). Additionally sequence of N. saginata  plus Floridosentis mugilis  and Floridosentis pacifica  were aligned together, composing a data set of 806 bp with 274 terminals. Nucleotide frequencies for 28 S rDNA data set were 0.246 (A), 0.178 (C), 0.276 (G), and 0.298 (T). Phylogenetic analyses of the available sequences for the genus Neoechinorhynchus  through the Bayesian consensus tree yielded the same branch pattern as the ML tree ( Fig. 3View FIGURE 3). Both trees yielded that the ten species of Neoechinorhynchus  are monophyletic with strong nodal support ranging from 72 to 100 % of bootstrap, and 1.0 of posterior probability ( Fig. 3View FIGURE 3). Both trees yielded three major clades. The first clade contained four species N. (N.) roseum  , N. saginata  , N. (N.) schmidti  and N. (N.) emyditoides  . The second clade was composed of two species N. (N.) golvani  and N. (N.) panucensis  and had 100 % bootstrap support and 1.0 posterior probability. The third clade was composed of four species N. (N.) chimalapasensis  , N. (N.) brentnickoli  , N. (N.) mamesi  and N. (N.) mexicoensis  and received reliable bootstrap support and high values of posterior probability ( Fig. 3View FIGURE 3).

67- 76 N. (N.) brentnickoli  10 D. latifrons Laguna  de Tres Palos, Guerrero, México FJ388991View Materials, 8178 FJ 968156View Materials - FJ 968159View Materials, KR086219View Materials - KR086223View Materials *

77- 81 N. (N.) brentnickoli  5 D. latifrons Río Tamarindo, Chautengo  , Guerrero, KR086224View Materials - KR086228View Materials * 8179 México

82 N.  (N.) roseum 1 Achirus mazatlanus Laguna  el Caimanero, Sinaloa, México FJ 388999View Materials 6762, 633, 634 83 N.  (N.) roseum 1 Citharichthys gilberti Estero La Tovara  , Nayarit, México FJ 389000View Materials 6763 84- 86 N. (N.) mamesi  3 D. latifrons Puente Manialtepec  , Oaxaca KR086229View Materials - KR086231View Materials *

......continued on the next page TABLE 1. (Continued)

Samples Species N Host Locality GenBank Specimensdeposited

( DNAAbout DNA) CNHE

87- 91 N. (N.) mamesi  5 D. latifrons Estero Joaquin Amaro  , Chiapas, México JN 830770View Materials - JN 830774View Materials 8183

92- 95 N. (N.) mamesi  4 D. latifrons Laguna  la Conquista, Chiapas, México JN 830766View Materials - JN 830769View Materials 8184

96- 98 N. (N.) mamesi  3 D. latifrons Laguna Rion Pijijiapan  , Chiapas, JN 830763View Materials - JN 830765View Materials 8180, 8181, 8182

México

99- 100 N. (N.) mamesi  2 D. latifrons Quebrada Ganados  , Costa Rica KR086232View Materials *, KR086233View Materials * 8590

101- 102 N. (N.) mamesi  2 D. maculatus Playa Grande  , Costa Rica KR086234View Materials *, 8191 KR086235View Materials *

103- 105 N. (N.) mamesi  3 D. latifrons Las  lisas, Guatemala KR086236View Materials - KR086238View Materials *

106- 107 N. (N.) golvani  1 Parachromis Lago  Jalapa, Costa Rica KR086239View Materials *

managuensis 

108- 109 N. (N.) golvani  2 Parachromis loisellei Lago  Jalapa, Costa Rica KR086240View Materials *,

KR086241View Materials *

110- 111 N. (N.) golvani  2 Amphilophus Lago  Jalapa, Costa Rica KR086242View Materials *, KR086243View Materials * 8592 longimanus

112 N.  (N.) golvani 1 Heterotilapia Lago  Jalapa, Costa Rica KR086244View Materials *

multiespinosa 

113- 114 N. (N.) golvani  2 Archocentrus Lago  Jalapa, Costa Rica KR086245View Materials *,

centrarchus  KR086246View Materials *

115- 116 N. (N.) golvani  2 Amatitlania Quebrada Puercos  , Costa Rica FJ388998View Materials, 6757 nigrofasciata  KR086247View Materials *

117- 127 N. (N.) golvani  11 Vieja pearsei Presa Chicoasen  , Chiapas, México FJ 388995View Materials, FJ 968136View Materials - FJ968138View Materials, 6755 KR086248View Materials - KR086254View Materials *

128- 132 N. (N.) golvani  5 V.  pearsei Presa Nezahualcoyolt, Chiapas, México FJ 388996View Materials, FJ 968141View Materials, FJ968142View Materials, 6756 KR086255View Materials *,

KR086256View Materials *

133- 143 N. (N.) golvani  11 Parachromis Lago Canitzan, Tenosique  , Tabasco, FJ 388994View Materials, FJ 968139View Materials, FJ968140View Materials, 6767 friedrichstalii  México KR086257View Materials - KR086264View Materials *

144- 153 N. (N.) golvani  10 Cichlasoma Río Carrizal  , Tabasco, México FJ 388993View Materials, FJ 968134View Materials, FJ968135View Materials, 6754 urophthalmum  KR086265View Materials - KR086271View Materials *

154- 156 N. (N.) golvani  3 C. urophthalmum Lago  las Ilusiones, Tabasco, México FJ 388992View Materials - FJ 968144View Materials

157- 167 N. (N.) golvani  11 Paraneetroplus Lago  de Catemaco, Veracruz, México FJ 388986View Materials, FJ 968145View Materials, FJ 968146View Materials, 601, 603, 604, 606, 631,

fenestratus  KR086272View Materials - KR086279View Materials * 632, 6783

168- 172 N. (N.) golvani  5 C. urophthalmum Presa Temascal  , Oaxaca, México KR086280View Materials - KR086284View Materials * 8593

......continued on the next page TABLE 1. (Continued) The sequence divergence estimated among the nine species herein considered, plus an additional sequence available for a species that occurs in freshwater fishes in North America ( N. saginata  ) ranged from 3.2 % to 48.7 % ( Table 2). Instead, the intraspecific genetic divergence varied markedly among all the isolates sequenced for each acanthocephalan species and it was never higher than 3 % for all intraspecific comparisons. For instance, the divergence among 81 specimens of N. (N.) brentnickoli  obtained from a wide geographic range, in 16 localities (localities 1-16 in Fig. 1View FIGURE 1) across the Pacific coast of northwestern Mexico, varied from 0 to 0.7 % ( Table 2). In other example, N. (N.) roseum  corresponding to two individuals of two localities 1) Laguna el Caimanero, Sinaloa and 2) Estero La Tovara, Nayarit (localities 17-18), had genetic divergence from 0 to 0.04 %. The species N. (N.) mamesi  comprises 22 individuals from seven localities from Mexico, one of Guatemala and two from Costa Rica (localities 19-25 in Fig. 1View FIGURE 1), showing a genetic divergence from 0 to 1.3 %. N. (N.) golvani  shows genetic divergence from 0 to 1.5 % among 80 specimens from 11 localities (see localities 26-36). The species N. (N.) mexicoensis  corresponding to 47 individuals from eight localities (localities 37-44) from Gulf of Mexico shows a genetic divergence ranged from 0 to 3 %. N. (N.) schmidti  shows low genetic divergence ranged from 0-0.02 % of specimens analyzed from the same locality. The species N. (N.) emyditoides  corresponding three localities: 1) Lago de Catemaco, Veracruz, 2) Río Papaloapan, Tlacotalpan, Veracruz and 3) Presa la Herradura, Monterrey (localities 46-48), showed a genetic divergence from 0 to1.3 %. The specimens of N. (N.) panucensis  are from Axtlan de Terrazas, San Luis Potosí, and Río Pantepec, Veracruz (localities 49-50), both localities are near of the type locality of N. (N.) panucensis  . The genetic divergence among four isolates of N. (N.) panucensis  ranged from 0.02–0.05 %. Finally, the two specimens of N. (N.) chimalapasensis  showed a genetic divergence equal to zero (locality 51).

1 2 3 4 5

1. N. (N.) brentnickoli  (n = 81) 0–0.7 42–43 8.1–9.8 21.9–23.7 7.3–10 2. N. (N.) roseum  (n = 2) 0–0.04 40.9–42.9 41.1–43.1 39.7–43.2 3. N. (N.) mamesi  (n = 22) 0–1.3 21–23.6 2.40–5.50 4. N. (N.) golvani  (n = 80) 0–1.5 21.0– 24.6 5. N. (N.) mexicoensis  (n = 47) 0–3.0 6. N. (N.) schmidti  (n = 5)

7. N. (N.) emyditoides  (n = 26)

8. N. (N.) panucensis  (n = 4)

9. N. (N.) chimalapasensis  (n = 2)

10. N. saginata  (n = 1)

continued.

TABLE 1. Specimens information: collection sites (CS), samples analyzed for DNA, species analyzed, specimens examined (N), host species, localities, GenBank accession number and catalog number (CNHE) for specimens studied in this work. Sequences marked with an asterisk (*) were obtained in the current study.

N. (N.) brentnickoli N. (N.) brentnickoli N. (N.) brentnickoli Dormitator latifrons D. latifrons D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons D. latifrons

TABLE 1. Specimens information: collection sites (CS), samples analyzed for DNA, species analyzed, specimens examined (N), host species, localities, GenBank accession number and catalog number (CNHE) for specimens studied in this work. Sequences marked with an asterisk (*) were obtained in the current study.

N. (N.) brentnickoli N. (N.) brentnickoli N. (N.) brentnickoli Dormitator latifrons D. latifrons D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons N. (N.) brentnickoli D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons N. (N.) brentnickoli N. (N.) brentnickoli N. (N.) brentnickoli D. latifrons D. latifrons D. latifrons
HWML

Howard W. Manter Laboratory of Parasitology

USNPC

United States National Parasite Collection

DNA

Department of Natural Resources, Environment, The Arts and Sport