Bolua turkiyae Ünal, 1999

Uluar, Onur, Yahyaoğlu, Özgül & Çiplak, Battal, 2022, Anatolian endemic genus Bolua (Orthoptera: Tettigoniidae: Tettigoniinae): genetic and phenotypic data indicate inconsistent diversity and evolutionary patterns, Zootaxa 5195 (3), pp. 224-240 : 230-232

Bolua turkiyae Ünal, 1999

( Figures 1–3, 6, 9, 12, 15 , 18, 21, 24 , 27 , 31 ; Tables 1–5 )

Bolua turkiyae Ünal ; 1999: 246

Type information: Holotype male, TURKEY: Bolu, Gerede, Aktas II, 1200 m, 21.08.1996, leg. M. Ünal (coll. M. Ünal in Abant Izzet Baysal University, Bolu, Turkey) . Paratypes 7 males, 11 females, same data as holotype ; 8 males, 5 females, same locality as holotype, 4.08.1995, leg M. Ünal (coll. M. Ünal in Abant Izzet Baysal University, Bolu, Turkey ) .

Material examined: 10 males, 8 females TURKEY: Çankırı, Çerkes, Isık Dağı, 1050–1250 m, 12.08.1996 (leg. B. Çıplak) (1 male, 1 female in The Natural History Museum , London (E-1997/322), others in AUZM) ; 2 males, 5 females, TURKEY: Kastamonu, Araç, Sıragömü yaylası, 900 m, 11.09.1998 (leg. B. Çıplak) ( AUZM) ; 4 males, 8 females, TURKEY, Bolu, Yedigöller National Park , 40.8804 °N, 31.68140°E, 1725m, 15.08.2010 (leg. B.Çıplak, S.Kaya & Z.Boztepe) ( MEVBIL) ; 16 males, 11 females, TURKEY, Kastamonu, Safranbolu, Karaevler , 41.34805°N, 32.69805°E, 996 m, 17.07.2017 (leg. B. Çıplak & O. Uluar) ( MEVBIL) ; 2 males, 2 females, TURKEY, Kastamonu, Şenpazar-Ağlı road, 41.724°N, 33.48833°E, 997m, 18.07.2017 ((leg. B. Çıplak & O. Uluar) ( AUZM) ; 2 males, 3 females, TURKEY, Kastamonu, Yenice , 41.23168°N, 33.79239°E, 1025m, 13.08.2004 (leg. D. Berger, D. Şirin, M.S. Taylan) ( MEVBIL) ; 1 male, 4 females; TURKEY, Karabük, Keltepe , 41.0555°N, 32.468°E, 1453m, 16.07.2017 (leg. B. Çıplak & O. Uluar)( MEVBIL) .

Description, diagnosis and remarks: Description of the type species Bolua turkiyae is given in Ünal (1999) and supplemented by Çıplak & Heller (2001). Data presented here (see Figures 3, 6, 9, 12, 15 , 18, 21, 24 , 27 ) are consistent with these descriptions. This species can be distinguished from B. balikesirensis sp. n. by male cercus (compare Figures 9 and 10 ). However, differences between B. turkiyae with B. bursaensis sp. n. are not prominent, but they can be identified by male cercus again (compare Figures 9 and 11 ). Apart from the qualitative morphology, the PCA analyses applied to female measurements distinguished B. turkiyae and B. bursaensis sp. n. from each other ( Figure 27 ). Along with morphological differences, B. turkiyae corresponding PG 1 in phylogenetic tree ( Figure 2 ) is definable by 53 unique mutations ( Figure 31 ). The high net pairwise distance and F ST values with the other two phylogroups also support its distinctness as a species ( Table 3 ).

Although Çıplak & Heller (2001) presented and described a male calling song for this species, that song record is from Uludağ— Bursa Province, the type locality of the new species described here, P. bursaensis sp. n. Presently song of this species is not available to study and to compare with the other two. This species occupies the eastern part of the generic range in the western part of the Anatolian Black Sea Basin ( Figure 1 ).

Figures

FIGURE 1. Distribution of Bolua [for detail of the localities see material examined per species; Bolu(1), Kastamonu (2,3,4, 6), Karabük (5), Çankırı (7), Balıkesir (8), Bursa (9, 10), Kütahya (11) and Kocaeli (12, 13)]

FIGURE 2. Phylogenetic tree, chronogram and taxonomic pattern obtained from the matrix constituted by concatenation of COI+NAD2+VAL+ITS sequences. The bootstrap (ML)/ posterior probability (BI) supports to the node are given above the node and the time to the most recent common ancestor (TMRCA) and the 95% HPD as million years below the node. The results from the species delimitation test are indicated by a bar line.

FIGURES 3–17. 3–5. Male head+pronotum+tegmina in Bolua (3—B. turkiyae, 4—B. balıkesirensis, 5—B. bursaensis; D— dorsal view, L—lateral view). 6–8. Male anal tergite in Bolua (6—B. turkiyae, 7—B. balıkesirensis, 8—B. bursaensis). 9–11. Male cercus in Bolua (9—B. turkiyae, 10—B. balıkesirensis, 11—B. bursaensis). 12–14. Male subgenital plate in Bolua (12—B. turkiyae, 13—B. balıkesirensis, 14—B. bursaensis). 15–17. Titillators in Bolua (15—B. turkiyae, 16—B. balıkesirensis, 17—B. bursaensis)

FIGURE 27. The PCA plots and trait contribution prepared from the metric data sets of male and female individuals belonging to Bolua

FIGURE 31. The ML tree showing unique base changes per phylogroups of Bolua, obtained by maximum parsimony analysis (the base position indicates by the position number in the concatenated matrix)

FIGURES 18–26. 18–20. Female head+pronotum+tegmina in Bolua (18—B. turkiyae, 19—B. balıkesirensis, 20—B. bursaensis; D—dorsal view, L—lateral view). 21–23. Female subgenital plate in Bolua (21—B. turkiyae, 22—B. balıkesirensis, 23—B. bursaensis; V—ventral view, L-lateral view). 24–26. Ovipositor in Bolua (24—B. turkiyae, 25—B. balıkesirensis, 26— B. bursaensis)