Triticum durum, wheat

Bouteraa, Mohamed Taieb, Romdhane, Walid Ben, Hsouna, Anis Ben, Amor, Fatma, Ebel, Chantal & Saad, Rania Ben, 2023, Genome-wide characterization and expression profiling of GASA gene family in Triticum turgidum ssp. durum (desf.) husn. (Durum wheat) unveils its involvement in environmental stress responses, Phytochemistry (113544) 206, pp. 1-13 : 10

4.2. Identification of TdGASA genes in durum wheat and chromosomal distribution

TdGASA protein sequences were identified from the Ensembl Plants database (http://plants.ensembl.org/index.html). The TaGASR protein sequences ( Cheng et al., 2019) were obtained from the Grain Genes database (https://wheat.pw.usda.gov/GG3) and were used as queries for TdGASA proteins search through the BLAST tool with an expected E-value threshold of E 50. The candidate TdGASA proteins were then screened using SMART (http://smart.embl-heidelberg.de/) to reveal the existence of the PF02704 GASA domain. Then, to guarantee that no TdGASA proteins were missed, the TdGASA sequences were carefully inspected and blasted one by one with a random reference AtGASA sequences; Roxrud et al., 2007). The chromosomal position of every TdGASA gene was drawn with the PhenoGram Plot tool (http://visua lization.ritchielab.org/phenograms/plot) by determining all the chromosomes’ lengths and each gene’ s location.