Anacroneuria flintorum Froehlich, 2002

Almeida, Lucas Henrique De, Gonçalves, Maísa De Carvalho, Novaes, Marcos Carneiro, Paresqui, Rayner Constantino & Bispo, Pitágor, 2018, Anacroneuria flintorum Froehlich 2002 (Plecoptera: Perlidae): Notes, distribution, and life stages association using molecular tools, Zootaxa 4370 (4), pp. 409-420: 411-415

publication ID

https://doi.org/10.11646/zootaxa.4370.4.6

publication LSID

lsid:zoobank.org:pub:5945C2DE-5B4E-496A-B576-50428EAAB90C

persistent identifier

http://treatment.plazi.org/id/03854734-FFC1-FFC4-A0E8-22D487B5F81A

treatment provided by

Plazi

scientific name

Anacroneuria flintorum Froehlich, 2002
status

 

Anacroneuria flintorum Froehlich, 2002 

Anacroneuria flintorum Froehlich, 2002: 93  ; Bispo & Froehlich, 2004: 99; De Ribeiro & Froehlich, 2007: 55; Froehlich, 2010: 56; Baldin et al., 2013: 394; Gonçalves et al., 2017: 146.

Material examined. BR, SP: Iporanga, Gruta da Tapagem, 15.xi.1991, 1 male; PEIAbout PEI, Rio do Carmo , 19.ii.1992, 1 male; PEIAbout PEI, Rio do Carmo, 16.ii.1993, 2 males; PEIAbout PEI, Rio Poços Altos, 10.xi.1993, CGF & HP col., 2 males; PETAR, Rio Betari, Bairro da Serra, 20.viii.1996, light trap, ASMAbout ASM col., 1 male; PEIAbout PEI, Córrego Lajeado , 24.ii.1999, 2 males; PEIAbout PEI, Córrego Lajeado, 17.ix.1999, 1 male; PEIAbout PEI, Ribeirão Água Comprida, 27.x.1999, 3 males; PEIAbout PEI, Rio do Carmo , 28.x.1999, 1 male; PEIAbout PEI, Ribeirão Água Comprida, 23.xi.1999, 7 males; PEIAbout PEI, Ribeirão Água Comprida, 06.i.2000, 8 males; PEIAbout PEI, Rio Poços Altos, 01.ii.2000, 2 males; PEIAbout PEI, Rio do Carmo, 25.iii.2000, 1 male; PEIAbout PEI, Ribeirão Água Comprida, 24°17’38”S, 48°25’04”W, 30.x.2002, ASMAbout ASM col., 1 male; PECB, Rio Taquaral , 09.ix.2006, 1 male; PEIAbout PEI, Córrego Roda D’Água, 13.xii.2014, light trap, PCB col., 1 female; PEIAbout PEI, Rio do Carmo, bridge, 24°18’15”S, 48°24’31”W, 14.xii.2014, light trap, PCB col., 1 male and 1 female; PEIAbout PEI, Córrego do Mirante   GoogleMaps, 15.xii.2014, light trap, PCB col., 1 female; PEIAbout PEI, Rio do Carmo, bridge, 24°18’15”S, 48°24’31”W, 08.ii.2017, light trap, LHA et al. col., 1 male; PEI, Ribeirão Bocaina, bridge, 24°16’13”S, 48°27’09”W, 12.ii.2017, light trap, LHA et al. col., 1 male; PETAR Núcleo Santana, Riacho Furnas, 24°32’03”S, 48°42’02”W, 14.ii.2017, light trap, LHA et al. col., 2 males; PETAR Núcleo Santana, Rio Roncador   GoogleMaps, 24°32’00”S, 48°42’06”W, 15.ii.2017, light pan trap, LHA et al. col., 1 male; PECB, Braço do Rio Taquaral   GoogleMaps, 24°03’31”S, 47°59’41”W, 05.ii.2017, D-net, LHA et al. col., 1 nymph; PECB, Ribeirão de Pedras, 24°03’40”S, 47°59’51”W, 06.ii.2017, D-net, LHA et al. col., 1 nymph; PECJ, Córrego Campo do Meio   GoogleMaps 22°41’28”S, 45°28’26”W, 28.i.2009, light trap, 2 males. BR, ES: Caparaó, Rio Santa Marta, 18.ii.2016, FFST col., 3 females and 7 nymph; Caparaó, Pedra Menina, Base, 14-15.ii.2016, FFST col., 2 females; Caparaó, Pedra Menina, Base, 15-16.ii.2016, FFST col., 6 females and 1 male. BR, MG: Araponga , Serra do Brigadeiro , Vale das Luas, 14.i.2014, FFST col. 1 nymph. BR, SC: Chapadão , 28#12’09”S, 49#23’14”W, 15.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male; Orleans, Rio da Serra, 28°12’43”S, 49#27’28”W, 16.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male.

Description and association of nymph. Molecular association. We associated adults with nymphs of A. flintorum  (including teneral adults) from the states of Espírito Santo and São Paulo. The COIAbout COI divergences ranged from 0–0.9% for specimens from the São Paulo State and from 0–0.7% from Espírito Santo State. Intraspecific divergences ranged from 0–3.9%, probably due the geographic variation. For all species studied, interspecific divergences ranged from 12.4%, between A. flintorum  (PLE481a) and A. sp. 2 ( PPIAbout PPI 15) to 20.6% between A. flintorum  (PLE479a) and A. debilis ( Pictet, 1841)  (PLE480b). A dendrogram and the matrix with pair divergence between specimens based on COIAbout COI sequences are presented in Fig. 1View FIGURE 1 and Table 2.

Description of nymph. General color dark brown in dorsal view and light yellow in ventral view. Head dark brown with light yellow areas near post-frontal suture, occiput and frontoclypeal region; M-line complete, with well-defined limits; eyes and ocelli black; ocelli with a translucent layer ( Fig. 2AView FIGURE 2); clypeus dark brown; labrum light yellow; maxillae light yellow with lacinia light brown and galea brown; mandible black with median region dark brown with f5 pointed teeth on distal margin, decreasing in size toward base, but teeth 3 and 4 similar in size ( Figs. 3A, 3B and 3CView FIGURE 3). Pronotum dark brown with thin median line and scattered rugosities light yellow ( Fig. 2AView FIGURE 2). Wing pad light with contour partially dark brown ( Fig. 2CView FIGURE 2). Leg brown, forefemur with a central pale line without setae and with lateral surface covered by sparse bristles, in dorsal view; forefemur anteriorly and posteriorly with higher density of thick bristles, and posteriorly with fringe of hairs; tibia anteriorly with sparse thick bristles, and posteriorly with a row of thick bristles and a well-developed band of hairs ( Figure 3DView FIGURE 3). Abdomen dark brown, almost black, with a drop-shaped light spot in the last segment ( Fig. 2BView FIGURE 2). Cerci brown with thick bristles; segments differ in shape and size from base to apex ( Fig. 3EView FIGURE 3). Thoracic gills ( Fig. 2DView FIGURE 2): ASCAbout ASC 1, AT2, AT3 and PT3.

Measurements (n=4): head width 3-5 mm; head length 2-3 mm; antennae length 7-8 mm; pronotum width 3-3.5 mm; pronotum length 1.5- 2 mm; cercal length 9-12 mm; total length 10-15mm.

Adult morphological variation. Examination of specimens from different regions of Atlantic Forest (states of Espírito Santo, Minas Gerais, Santa Catarina, and São Paulo) indicated morphological variation. Forewing length of male adults of A. flintorum  range from 10 to 15.2 mm (specimens studied herein and Bispo & Froehlich, 2004; Froehlich, 2010, and Baldin et al., 2013). The dorsal color pattern of head of adult of A. flintorum  can be variable, even within the same population ( Bispo & Froehlich, 2004). In addition, it is possible to observe that the dark brown color of recently collected specimens can become ochraceous when preserved in 80% and 100% ethanol for an extended period of time ( Fig. 4View FIGURE 4). Therefore, it is difficult to identify the species by color pattern alone. For identification, it is essential to study the male penial armature, which in A. flintorum  is elongated, tapering progressively from base to the apex and has hooks well separated from distal vesicles ( Froehlich, 2002; Bispo & Froehlich, 2004). However, the keel of penial armature may diverge in specimens from the same population and in different populations. In dorsal view, the keel varies from rounded (male specimens from Rio de Janeiro State), pointed (male specimens from PECJ), to convergent or parallel lines (male specimens from PEIAbout PEI). The specimens collected farther north of the species distribution (e. g. ES, RJ and North of SP) have penial armature more robust than specimens from further south (e.g. South of SP and SC).

Teneral adult specimens. Teneral specimens of A. flintorum  studied are generally brown in color with pale areas along the body; head and pronotum brown with a pale outline. In these specimens, the pronotum does not have lateral folds at the edges, wings are totally pale and the thoracic segments present remnants of gills ( Figure 5D, 5E and 5FView FIGURE 5). A similar pattern to A. flintorum  is also present in A. debilis ( Almeida & Duarte, 2017)  suggesting that several species may be similar when teneral. Therefore, species described as having pale areas on the head and pronotum and milky wings (e.g. A. petersi Froehlich, 2002  ) may have been based on teneral specimens, requiring caution for use in identifications.

Remarks. The nymph of A. flintorum  has a different pattern than the other known Anacroneuria  nymphs described from Brazil ( Righi-Cavallaro & Lecci, 2010; Avelino-Capistrano et al., 2011a; Novaes et al., 2012; Righi-Cavallaro & Froehlich, 2013; Ribeiro & Gorayeb, 2014; Gonçalves et al., 2017). The color pattern of the head and the drop-shaped light spot in the last abdominal segment are apparently characteristic for this species. In addition to description of the nymph, we present new species records from the states of Minas Gerais and Santa Catarina. Anacroneuria flintorum  has been collected from streams from protected areas of the Atlantic Forest ( Fig. 6View FIGURE 6). In general, these streams are pristine and have fast currents and rocky substrates, and undisturbed channels and banks. This suggests that A. flintorum  may be a sensitive taxon to environmental disturbances.

PEI

Agriculture and Agri-Food Canada

ASM

Arts and Science University

COI

University of Coimbra Botany Department

PPI

National Pingtung University of Science and Technology

ASC

Northern Arizona University

Kingdom

Animalia

Phylum

Arthropoda

Class

Insecta

Order

Plecoptera

Family

Perlidae

Genus

Anacroneuria

Loc

Anacroneuria flintorum Froehlich, 2002

Almeida, Lucas Henrique De, Gonçalves, Maísa De Carvalho, Novaes, Marcos Carneiro, Paresqui, Rayner Constantino & Bispo, Pitágor 2018

2018
Loc

Anacroneuria flintorum

Froehlich 2002: 93

2002