Trametopsis Tomšovský emend. Gómez-Montoya, Robledo & Tomšovský

Gómez-Montoya, Nataly, Tomšovský, Elisandro Ricardo Drechsler-Santos Valéria Ferreira-Lopes Michal, Urcelay, Carlos & Robledo, Gerardo Lucio, 2017, New insights on Trametopsis Tomšovský (Polyporales Gäum) based on phylogenetic evidences and morphological analyses of neotropical species, Phytotaxa 311 (2), pp. 155-167 : 159-164

Trametopsis Tomšovský emend. Gómez-Montoya, Robledo & Tomšovský

Czech Mycol. 60(1): 7 (2008)

Type species: Trametopsis cervina Tomšovský , Czech Mycol. 60(1): 8 (2008)

Basidiomata annual, sessile pileated to effused-reflexed or rarely resupinate, usually in imbricate clusters; pilear surface smooth, glabrous to strigose, pinkish buff to cinnamon or clay colour, usually zonate sometimes azonate; pore surface concolorous with upper surface, darkening when dry; pores angular, irregular, daedaloid to irpicoid, dissepiments becoming thin and lacerate, usually large 1–4 per mm; context fibrous, azonate. Hyphal system dimitic, hyphal walls metachromatic, negative in Melzer’s reagent and CB. Context monomitic with clamped, thin-walled, thick-walled to sclerified generative hyphae, forming short or rather long intercalary segments. Trama of the tubes dimitic with hyaline, thin-walled generative hyphae and thick-walled skeletal hyphae. Basidia clavate. Fusoid cystidioles occasionally present. Basidiospores cylindrical and slightly curved to allantoid, hyaline, thin-walled, negative in Melzer’s reagent and CB.

Remarks:—the combination of the microscopic characters of Trametopsis perfectly fits the description of Trametes cervina by Jahn (1983, cf. figure 8–9), for which three types of hyphae are described: a) thin-walled, hyaline, clamped generative hyphae with numerous oil drops and often with short branches; b) thick-walled clamped generative hyphae with a thin lumen and intercalary segments of thick-walled to mostly solid, in between thin walled hyphae and c) thick-walled skeletal hyphae.

Trametopsis aborigena Gómez-Montoya & Robledo sp. nov. ( Figure 2A–D , 3A–C, F , 4 ) MycoBank: MB 820105 Typification:— ARGENTINA. Salta: Dpto. Sta. Victoria Oeste, Parque Nacional Baritú, Lipeo (to Baritú), 1222 m. a. s. l., 22º 26’ 21.6”

S, 64º 44’ 7.8” W, on dead tree, 6 May 2007, Robledo 1236 [ FCOS 41, CORD ( C)00005352] GenBank accession numbers ITS:

KY 655336, LSU: KY 655338.

Etymology:—from latin “ aborigines” = aboriginal, native to the area.

Description:— Basidiomata annual, sessile, pileate, effused-reflexed to occasionally resupinate, pileus dimidiate and broadly attached, applanate and lightly convex, occasionally imbricate, each pileated portion up 5 × 1.5 × 0.5 cm, the small resupinate part under the pileus effused up 10 × 5 × 0.2 cm. Pilear surface light pale brown (7.5YR 7/6–8/4) toward the base and close to the substrate, changing to pale yellowish (10YR 8/3–8/4) [or some specimens strong brown (5YR 5/8)] near the margin, velutinate towards the base, with yellowish red (2.5YR 4/8) to dark yellowish brown (7.5YR 8/3–8/4) radial veins composed of hyphal fascicles and branching to the margin. Margin yellowish (7.5YR 7/6), in section round in the pileate portion, irregular in the effused portion, up 1 mm wide. Pore surface pale brown (7.5YR 7/6–8/4) to pale yellow (10YR 8/3–8/4). Pores angular, irregular, 1–3 per mm, some pores are fused giving a daedaloid appearance. Dissepiments irregular in height, lacerate to slightly irpicoid. Context homogeneous, whitish (10YR 8/1), fibrous, up to 0.4 cm thick at the base in the pileate portion, 0.1 cm thick in the effused portion. Tube layer not stratified, white (10YR 8/1) to pale yellow (10YR 8/3–8/4), up to 0.1 cm thick at the base ( Figure 2 ).

Hyphal system monomitic in the context and dimitic in the trama of the tubes, IKI-, CB-, metachromatic; contextual generative hyphae clamped, 3.0–4.5 μm wide, of two types, a) hyaline and thin walled with numerous oil drops, and b) pale yellowish thick-walled with a thin lumen, often with short lateral solid branches, intercalary segments of thick-walled, mostly solid, in between thin-walled hyphae, thick-walled hyphae dominate the context giving a dimitic appearance, trama of the tubes with clamped generative hyphae mostly thin-walled, skeletal hyphae thick-walled with a wide lumen, to 900 μm long, 3.9 μm wide in the main part, ending with rounded apices. Cystidia absent. Basidia clavate, with basal clamp, hyaline, thin-walled, 19–22 × 5–6 μm, with four sterigmata. Basidiospores subalantoid in equatorial lateral view and cylindrical in equatorial adaxial view and equatorial abaxial view, thin-walled, hyaline, IKI-, 5–7 (–7.5) × 1–2.5 μm, = 6.3 × 1.7 μm, Q =3.5–4.2, = 3.9.

Ecology and distribution:—growing on fallen branches, so far known from the Yungas Mountain Rain Forests in NW Argentina. For a detailed description of the Yungas forests and its polypore diversity see Robledo & Rajchenberg (2007) and references therein.

Remarks:— Trametopsis aborigena is characterized by pileate basidiomata, with yellowish red to dark yellowish brown radial veins on the pilear surface, angular and irregular pores 1–3 per mm and suballantoid to cylindrical basidiospores, 5–7 × 1–2 μm. Macromorphologically, it resembles T. cervina , however the basidia and basidiospores of T. cervina are larger than those of T. aborigena ( Table 2).

Specimens examined:— ARGENTINA. Salta: Dpto. Sta. Victoria West, Parque Nacional Baritú-Lipeo-(to Baritú), elevation 1222 m. a. s. l., 22º 26’ 21.6” S, 64º 44’ 7.8” W, growing on dead tree, 6 May 2007, ROBLEDO 1238. [ FCOS 42, CORD ( C)00005353]

Additional specimens examined:— Trametopsis cervina : CZECH REPUBLIC. Valašské Meziříčí, Oznice village, 49. 4380556º N, 017.9066667º E, growing on wood of Fagus sylvatica , 22 October 2010, leg. V. Tomešová, det. M. Tomšovský (FCOS-39); Ibid., Moravian Karst Protected Nature Area, Habrůvka Bučina National Nature Reserve, 49.3192028ºN, 16.6944983º E, growing on a fallen Fagus sylvatica , 14 January 2016, leg. J. Hrabáková, det. M. Tomšovský (FCOS-40).

Trametopsis brasiliensis (Ryvarden & de Meijer) Gómez-Montoya & Robledo comb. nov. ( Figures 2 H–J , 3H ). Mycobank: MB 820117

Basiomyn: Antrodiella brasiliensis Ryvarden & de Meijer , Synopsis Fungorum 15: 40, 2002 ( HF!)

Remarks:—The study of the type and paratype specimens showed a context composed of thin to thick-walled clamped generative hyphae; in the tubes, generative hyphae are thin-walled and skeletal hyphae are thick-walled to solid with a wide lumen. Basidiospores are allantoid, 5–5.5(–6) × 2.0–2.5 (3.0) μm, slightly larger than originally described [4.5–5.5 (–6) × 1.8–2.2 μm]. Pores are angular, irregular, with some pores fused, sinuous to lamellate ( Table 2).

Specimens examined:— BRAZIL. Paraná, Colombo, Embrapa Florestas, 900 m. a. s. l., growing on decayed dicotyledonous branch, 28 May 1999, HF C 3637 (holotype). Ibid., Campina Grande do Soul, Parque Marumbi, ombrophilus forest, on decayed dicotyledonous branch, 1000 m.a.s.l., 7 May 1992, A de Meijer 2248, MBM 084739.

Trametopsis luteocontexta (Ryvarden & de Meijer 2002) Gómez-Montoya, Robledo & Drechsler-Santos comb. nov. ( Figures 2K–M , 3I ).

Mycobank: MB 820118

Basionym: Antrodiella luteocontexta Ryvarden & de Meijer , Synopsis Fungorum 15: 41, 2002 ( MBM!).

Remarks:—The study of the type specimen showed that the context present generative hyphae thin-walled to thick-walled, both with clamps; in the tubes, generative hyphae thick-walled to thin-walled, both with clamps and skeletal hyphae thick-walled to solid with a wide lumen. Basidiospores are cylindrical, 4.5–5.0 × (1.0–)1.5–2.0 μm, larger than originally reported [3.0–3.5(–4) × 1.4–2.0]. Pores are round to angular, irregular, entire, 1–2(–3) per mm.

Specimens examined:— BRAZIL. Paraná, Curitiba, Parque Barigui, in mixed ombrophilous forest, 900 m. a. s. l., growing on decayed dicotyledonous branch, A. de Meijer 2574, 5 March 1993, MBM 180795.

Figures

FIGURE 2. Macroscopic features of Trametospis species. Trametopsis aborigena A–D [HOLOTYPE FCOS41, CORD(C 00005352], A) Pileus, B) Context, C) Reddish veins pileal surface, D) Pores. Trametopsis cervina E–G (FCOS-39), E) Pileus, F) Context, G) Pores. Trametopsis brasiliensis H–J (HOLOTYPE, HF C3637), H) Pileus, I) Context, J) Pores. Trametopsis luteocontexta K–M (HOLOTYPE, MBM 180795), K) Pileus, L) Context, M) Pores. Bars = 0.5 cm. Photos by: Nataly Gómez-Montoya and Gerardo Robledo

FIGURE 3. Microscopic characters of Trametopsis aborigena A–C, F [HOLOTYPE FCOS41, CORD(C) 00005352]. A–C) Clamped generative hyphae, F) Basidiospores. Trametopsis cervina D–E, G (FCOS39) D) Generative hyphae clamped, E) Metachromatic, clamped generative hyphae, G) Basidiospores. Trametopsis.brasiliensis (HOLOTYPE, HF C3637) H) Basidiospores. Trametopsis luteocontexta (HOLOTYPE, MBM 180795) I) Basidiospores. Bars = 5 μm. Photos by: Nataly Gómez-Montoya

FIGURE 4. Microscopic features of Trametopsis aborigena [HOLOTYPE FCOS41, CORD(C) 00005352]. A) Sporogram from the basidiospores in equatorial and polar view, (el = equatorial lateral view; ead = equatorial adaxial view; eab = equatorial abaxial view; dp = distal polar view and pp = proximal polar view). B) Basidia, C) Thick and thin-walled generative hyphae from the context, D) Skeletal hyphae in the trama of the tubes. Drawings by Nataly Gómez-Montoya.